PROTAMINE1 and PROTAMINE2 genes expression in the sperms of oligoasthenospermic individuals and intrauterine insemination candidates couples: Is there any significant differences?

Aboutorabi, Roshanak; Asghari, Mohsen; Bakhteyari, Abbas; Baghazadeh, Shokoofeh; Mostafavi, Fatemeh Sadat

PROTAMINE1 and PROTAMINE2 genes expression in the sperms of oligoasthenospermic individuals and intrauterine insemination candidates couples: Is there any significant differences?

Document Type : Original Article

Authors

¹ Department of Anatomical Sciences and Molecular Biology, School of Medicine; Department of Obstetrics and Gynecology, Infertility Laboratory, Beheshti Hospital, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

² Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

³ Department of Obstetrics and Gynecology, Infertility Laboratory, Beheshti Hospital, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Male infertility refers to a male's inability to cause pregnancy in a fertile female. It seems the large portion of this category of infertility, has roots in genetic factors. PROTAMINE family is one of the most important genes which are involved in male factor infertility. Hence, the aim of this study is to evaluate PROTAMINE1 and PROTAMINE2 (P1 and P2) genes expression in oligoasthenospermic individuals and intrauterine insemination (IUI) candidate couples' sperms.
Materials and Methods: Samples were gathered from the patients referred to the Isfahan Infertility Center of Shahid Beheshti, 80 semen samples were in IUI candidates groups and 16 semen samples were in oligoasthenospermia group was collected. The outcome of IUI procedure was followed up after 14 days. Through these samples, 16 couples achieved pregnancy (IUI+) and from the top of the list, 16 semen samples with negative β-HCG were obtained (IUI−). After RNA extraction from sperms, PROTAMINE genes family expression was evaluated in our three groups by real time-reverse transcription polymerase chain reaction.
Results: Our study revealed that P1 gene expression has no significant differences between IUI−, IUI+, and oligoasthenospermia groups, whereas P2 gene expression showed significant differences between oligoasthenospermia with two IUI groups. Main sperm parameters have no significant differences between IUI groups.
Conclusion: This study reveals P1 and P2 genes expression value have no significant differences between IUI− and IUI+. On the other hand, P2 gene expression value has significant differences between oligoasthenospermia with two IUI groups.

Keywords

References

1.	Alizadeh F, Vegar MI, Koosha S, Fesharaki M. Studing the effects of psychological conclusion on the sense of the life among the reffering infertile woman to the infertility center of tabriz in 2010. Int J Acad Res 2011;3:420-4.
2.	Parsanezhad M, Jahromi B, Zare N, Keramati P, Khalili A. Epidemiology and etiology of infertility in Iran, systematic review and meta-analysis. J Womens Health 2013;6:2.
3.	Ortega C, Verheyen G, Raick D, Camus M, Devroey P, Tournaye H. Absolute asthenozoospermia and ICSI: What are the options? Hum Reprod Update 2011;17:684-92. [PUBMED]
4.	Serour G. Medical and socio-cultural aspects of infertility in the Middle East. ESHRE Monogr 2008;2008:34-41.
5.	Ford WC, North K, Taylor H, Farrow A, Hull MG, Golding J. Increasing paternal age is associated with delayed conception in a large population of fertile couples: Evidence for declining fecundity in older men. The ALSPAC Study Team (Avon longitudinal study of pregnancy and childhood). Hum Reprod 2000;15:1703-8. [PUBMED]
6.	Olooto W. Infertility in male; risk factors, causes and management – A review. J Microbiol Biotechnol Res 2012;2:641-5.
7.	Carosi L, Calabrò F. Fertility in couples working in noisy factories. Folia Med (Napoli) 1968;51:264-8.
8.	Selevan SG, Borkovec L, Slott VL, Zudová Z, Rubes J, Evenson DP, et al. Semen quality and reproductive health of young Czech men exposed to seasonal air pollution. Environ Health Perspect 2000;108:887-94.
9.	De Gennaro L, Balistreri S, Lenzi A, Lombardo F, Ferrara M, Gandini L. Psychosocial factors discriminate oligozoospermic from normozoospermic men. Fertil Steril 2003;79 Suppl 3:1571-6. [PUBMED]
10.	Uehara S, Hashiyada M, Sato K, Sato Y, Fujimori K, Okamura K. Preferential X-chromosome inactivation in women with idiopathic recurrent pregnancy loss. Fertil Steril 2001;76:908-14. [PUBMED]
11.	Costabile RA, Spevak M. Characterization of patients presenting with male factor infertility in an equal access, no cost medical system. Urology 2001;58:1021-4. [PUBMED]
12.	Skakkebaek NE, Giwercman A, de Kretser D. Pathogenesis and management of male infertility. Lancet 1994;343:1473-9. [PUBMED]
13.	Cavallini G. Male idiopathic oligoasthenoteratozoospermia. Asian J Androl 2006;8:143-57.
14.	Hauser R, Meeker JD, Singh NP, Silva MJ, Ryan L, Duty S, et al. DNA damage in human sperm is related to urinary levels of phthalate monoester and oxidative metabolites. Hum Reprod 2007;22:688-95. [PUBMED]
15.	Oger I, Da Cruz C, Panteix G, Menezo Y. Evaluating human sperm DNA integrity: Relationship between 8-hydroxydeoxyguanosine quantification and the sperm chromatin structure assay. Zygote 2003;11:367-71. [PUBMED]
16.	Honig SC, Lipshultz LI, Jarow J. Significant medical pathology uncovered by a comprehensive male infertility evaluation. Fertil Steril 1994;62:1028-34. [PUBMED]
17.	Shefi S, Turek PJ. Definition and current evaluation of subfertile men. Int Braz J Urol 2006;32:385-97. [PUBMED]
18.	Bhasin S, Cunningham GR, Hayes FJ, Matsumoto AM, Snyder PJ, Swerdloff RS, et al. Testosterone therapy in men with androgen deficiency syndromes: An endocrine society clinical practice guideline. J Clin Endocrinol Metab 2010;95:2536-59. [PUBMED]
19.	Rockett JC, Patrizio P, Schmid JE, Hecht NB, Dix DJ. Gene expression patterns associated with infertility in humans and rodent models. Mutat Res 2004;549:225-40. [PUBMED]
20.	Lewis SE, Aitken RJ. DNA damage to spermatozoa has impacts on fertilization and pregnancy. Cell Tissue Res 2005;322:33-41. [PUBMED]
21.	Cooper TG, Noonan E, von Eckardstein S, Auger J, Baker HW, Behre HM, et al. World Health Organization reference values for human semen characteristics. Hum Reprod Update 2010;16:231-45. [PUBMED]
22.	Lopes S, Jurisicova A, Sun JG, Casper RF. Reactive oxygen species: Potential cause for DNA fragmentation in human spermatozoa. Hum Reprod 1998;13:896-900. [PUBMED]
23.	Yu B, Qi Y, Liu D, Gao X, Chen H, Bai C, et al. Cigarette smoking is associated with abnormal histone-to-protamine transition in human sperm. Fertil Steril 2014;101:51-7.e1.
24.	Balhorn R. A model for the structure of chromatin in mammalian sperm. J Cell Biol 1982;93:298-305. [PUBMED]
25.	Nasr-Esfahani MH, Aboutorabi R, Razavi S. Credibility of chromomycin A3 staining in prediction of fertility. Int J Fertil Sci 2009;3:5-10.
26.	WHO. 2010. Laboratory manual for the examination of human semen and sperm–cervical mucus interaction. (5^th ed.). Cambridge University Press: Cambridge, United Kingdom.
27.	Nasr-Esfahani MH, Salehi M, Razavi S, Mardani M, Bahramian H, Steger K, et al. Effect of protamine-2 deficiency on ICSI outcome. Reprod Biomed Online 2004;9:652-8. [PUBMED]
28.	Oliva R. Protamines and male infertility. Hum Reprod Update 2006;12:417-35. [PUBMED]
29.	Nasr-Esfahani MH, Razavi S, Mardani M, Shirazi R, Javanmardi S. Effects of failed oocyte activation and sperm protamine deficiency on fertilization post-ICSI. Reprod Biomed Online 2007;14:422-9. [PUBMED]
30.	Tavallaee M, Stakhanova N, Ghorbani AA. Toward credible evaluation of anomaly-based intrusion-detection methods. Systems, man, and cybernetics, part C: Applications and reviews. IEEE Trans 2010;40:516-24.
31.	Hammoud SS, Low DH, Yi C, Carrell DT, Guccione E, Cairns BR. Chromatin and transcription transitions of mammalian adult germline stem cells and spermatogenesis. Cell Stem Cell 2014;15:239-53. [PUBMED]
32.	Shokri S, Hemadi M, Bayat G, Bahmanzadeh M, Jafari-Anarkooli I, Mashkani B. Combination of running exercise and high dose of anabolic androgenic steroid, nandrolone decanoate, increases protamine deficiency and DNA damage in rat spermatozoa. Andrologia 2014;46:184-90. [PUBMED]
33.	Raman RS, Chan PJ, Corselli JU, Patton WC, Jacobson JD, Chan SR, et al. Comet assay of cumulus cell DNA status and the relationship to oocyte fertilization via intracytoplasmic sperm injection. Hum Reprod 2001;16:831-5. [PUBMED]
34.	Duty SM, Singh NP, Ryan L, Chen Z, Lewis C, Huang T, et al. Reliability of the comet assay in cryopreserved human sperm. Hum Reprod 2002;17:1274-80. [PUBMED]
35.	Iranpour FG, Nasr-Esfahani MH, Valojerdi MR, Al-Taraihi TM. Chromomycin A3 staining as a useful tool for evaluation of male fertility. J Assist Reprod Genet 2000;17:60-6. [PUBMED]
36.	Nasr-Esfahani MH, Razavi S, Mardani M. Andrology: Relation between different human sperm nuclear maturity tests and in vitro fertilization. J Assist Reprod Genet 2001;18:219-25. [PUBMED]
37.	Fawcett DW, Anderson WA, Phillips DM. Morphogenetic factors influencing the shape of the sperm head. Dev Biol 1971;26:220-51. [PUBMED]
38.	Sotolongo B, Lino E, Ward WS. Ability of hamster spermatozoa to digest their own DNA. Biol Reprod 2003;69:2029-35. [PUBMED]
39.	Szczygiel MA, Ward WS. Combination of dithiothreitol and detergent treatment of spermatozoa causes paternal chromosomal damage. Biol Reprod 2002;67:1532-7. [PUBMED]
40.	Alvarez JG, Sharma RK, Ollero M, Saleh RA, Lopez MC, Thomas AJ Jr., et al. Increased DNA damage in sperm from leukocytospermic semen samples as determined by the sperm chromatin structure assay. Fertil Steril 2002;78:319-29.
41.	Irvine DS, Twigg JP, Gordon EL, Fulton N, Milne PA, Aitken RJ. DNA integrity in human spermatozoa: Relationships with semen quality. J Androl 2000;21:33-44. [PUBMED]
42.	Oliva R, Dixon GH. Vertebrate protamine genes and the histone-to-protamine replacement reaction. Prog Nucleic Acid Res Mol Biol 1991;40:25-94. [PUBMED]

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