Advanced Biomedical Research
Gas chromatography–mass spectrometry analysis of Anbarnesa smoke and its antiviral activity
Authors
1 Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
2 Department of Pharmacology, School of Medicine, Medical Plants Research Center Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
Abstract
Background: Anbarnesa is the female donkey dung typically collected after the labor and in early springtime.
Materials and Methods: The chemical composition of the smoke collected from Anbarnesa was evaluated by gas chromatography–mass spectrometry (GC/MS), and its antiviral activity was analyzed based on 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay.
Results: As a result, twenty-two constituents representing 97.1% of the Anbarnesa smoke could be identified. Hexadecanoic acid (29.4%), cis-9-octadecenoic acid (17.7%), and octadecanoic acid (10.8%) were the smoke's main constituents, respectively. Antiviral activity was evaluated using MTT assay. The CC50 value of the compound on Hep2 and Verro cells was 2271.2 μg/mL and 5077.5 μg/mL, respectively. Furthermore, the 50% inhibitory concentration value on adenovirus and herpes simplex type-1 was 802.55 μg/mL and >5077.5, respectively.
Conclusions: it was revealed that Anbarnesa was nontoxic in 1/64, 1/128, and 1/256 dilutions, while the toxicity was detected in 1/32 dilution after 72 h. In addition, in 1/8 and 1/16 dilutions, cell toxicity was identified in the first hour.
Keywords
| 1. |
Fabricant DS, Farnsworth NR. The value of plants used in traditional medicine for drug discovery. Environ Health Perspect 2001;109 Suppl 1:69-75.  |
| 2. |
Taheri JB, Azimi S, Rafieian N, Zanjani HA. Herbs in dentistry. Int Dent J 2011;61:287-96. |
| 3. |
Mohagheghzadeh A, Faridi P, Shams-Ardakani M, Ghasemi Y. Medicinal smokes. J Ethnopharmacol 2006;108:161-84. |
| 4. |
Firouz SM. Effects of ANNAS1 Liquid on the Incisional Wound Healings in Rats. Tehran: Shahid Beheshti University of Medical Sciences; 2011. |
| 5. |
Shafiee HA, Motamedi MH, Mina M, Taheri JB, Azimi S, Joharchi K, et al. Evaluation of cytotoxic effects of Anbarnesa on fibroblast L929: Can it be used as a mouthwash? Anc Sci Life 2014;33:203-7. |
| 6. |
Shafiee H, Mortazavi H, Baharvand M, Eslami G, Bakhtiari S, Taheri S. Iranian traditional medicine: Comparison of the antibacterial effect of ANNAS 0.2% and chlorhexidine 0.2%. Afr J Microbiol Res 2012;6:3600-3. |
| 7. |
Avicenna HA. The Canon. 2nd, 3rd ed. Tehran: Soroush Press; 2005. p. 142-282, 302. |
| 8. |
Rhazi MZ. Al-Hawi. Tehran: Publications of the Academy of Medical Sciences; 2005. p. 195. |
| 9. |
Nejadi VA. Iran: Teb SHie; Anbarnesa. Available from: http://shiateb.com/[Last accessed on 2019 Apr 20]. |
| 10. |
Sweetman SC. Dose adjustment in renal impairment: Response from Martindale: The Complete Drug Reference. BMJ 2005;331:292-3. |
| 11. |
Parvin N, Validi M, Banitalebi M, Mobini G, Ashrafi K, Farrokhi E, et al. Effect of medicinal smokes on some nosocomial infection factors. J Shahrekord Uni Med Sci 2010;12:76-83. |
| 12. |
Ahmadian-Attari MM, Amrollahi Z, Momeni PS, Khodaii Z, Bita'ab A, Bozorgi AH. Chemical constituents of donkey dung (Anbarnasara): Questioning the recent claims concerning therapeutic effects. Int J Enteric Pathog 2019;7:19-22. |
| 13. |
Sadeghi-Aliabadi H, Saremi M, Mirian M, Ghannadi A. Cytotoxic evaluation of medicinal smoke. Yafteh 2013;15:87-94. |
| 14. |
Aghili Khorasani Shirazi MH. Makhzan al-Advyeh. Tehran: Tehran University of Medical Sciences; 2008. pp. 62. |
| 15. |
Afsharipour S. A Review of Traditional Iranian Drug Dosage Forms.Tehran (Iran): Chogan Publisher 2020. |
| 16. |
Adams RP. Identification of Essential Oil Components by Gas Chromatography/Mass Spectroscopy. Illinois: Allured Publishing Corpo-Ration; 1995. p. 69-351. |
| 17. |
Swigar AA, Silverstein RM. Monoterpenes. In: Infrared, Mass, 1H-NMR, 13C-NMR Spectra and Kovats Indices. Wisconsin: Aldrich Chemical Company; 1981. p. 1-121. |
| 18. |
Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63. |
| 19. |
Jadhav P, Kapoor N, Thomas B, Lal H, Kshirsagar N. Antiviral potential of selected Indian medicinal (ayurvedic) plants against herpes simplex virus 1 and 2. N Am J Med Sci 2012;4:641-7.
|
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