Advanced Biomedical Research

Prevalence of Helicobacter pylori vacA different genotypes in Isfahan, Iran

Authors

1 Department of Microbiology, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran

2 Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

3 Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

4 Department of Cell Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

5 Department of Gastroenterology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: It is believed that the Helicobacter pylori (H. pylori) vacA gene, as a major virulence determinant (One of the major virulence determinant, not major), may be a risk factor for the development of gastroduodenal diseases. The frequency of vacA genotypes varies in different human populations. This study evaluated the prevalence of vacA alleles/genotypes among dyspeptic patients in Isfahan.
Materials and Methods: One-hundred H. pylori-positive adult patients were examined in this study. After culture of gastric biopsies and DNA extraction from individual H. pylori isolates, the (all H. pylori strains harbor vacA alleles, please replace "presence" with "genotypes") of the vacA s and m alleles were determined using polymerase chain reaction (PCR).
Results: There were four vacA mosaicisms, including 28 for s1a/m1 (28%), 23 for s1b/m1 (23%), 26 for s1a/m2 (26%) and 23 for s1b/m2 (23%). The s2 allele was not found. The predominant vacA genotype in patients with non-ulcer dyspepsia and duodenal ulcer was s1a/m2, whereas in patients with adenocarcinoma was s1a/m1.
Conclusion: The results showed there was no significant correlation between different genotypes of the vacA and the clinical outcomes and appears to vacA genotypes were not useful determinants for gastrointestinal diseases in our area.

Keywords

1. Blaser MJ. Ecology of Helicobacter pylori in the human stomach. J Clin Invest 1997;100:759-62.  Back to cited text no. 1
[PUBMED]    
2. Blaser MJ. Helicobacter pylori and the pathogenesis of gastroduodenal inflammation. J Infect Dis 1990;161:626-33.  Back to cited text no. 2
[PUBMED]    
3. Dunn BE, Cohen H, Blaser MJ. Helicobacter pylori. Clin Microbiol Rev 1997;10:720-41.  Back to cited text no. 3
[PUBMED]    
4. Kumar S, Kumar A, Dixit VK. Direct detection and analysis of vacA genotypes and cagA gene of Helicobacter pylori from gastric biopsies by a novel multiplex polymerase chain reaction assay. Diagn Microbiol Infect Dis 2008;62:366-73.  Back to cited text no. 4
    
5. Forsyth MH, Atherton JC, Blaser MJ, Cover TL. Heterogeneity in levels of vacuolating cytotoxin gene (vacA) transcription among Helicobacter pylori strains. Infect Immun 1998;66:3088-94.  Back to cited text no. 5
[PUBMED]    
6. Boyanova L, Markovska R, Yordanov D, Marina M, Ivanova K, Panayotov S, et al. High prevalence of virulent Helicobacter pylori strains in symptomatic Bulgarian patients. Diagn Microbiol Infect Dis 2009;64:374-80.  Back to cited text no. 6
[PUBMED]    
7. Yamaota Y, Kato M, Asaka M. Geographic differences in gastric cancer incidence can be explained by differences between Helicobacter pylori strains. Intern Med 2008;47:1077-83.  Back to cited text no. 7
    
8. Massarrat S, Saberi-Firoozi M, Soleimani A, Himmelmann GW, Hitzges M, Keshavarz H. Peptic ulcer disease, irritable bowel syndrome and constipation in two populations in Iran. Eur J Gastroenterol Hepatol 1995;7:427-33.  Back to cited text no. 8
[PUBMED]    
9. Siavoshi F, Malekzadeh R, Daneshmand M, Ashktorab H. Helicobacter pylori endemic and gastric disease. Dig Dis Sci 2005;50:2075-80.  Back to cited text no. 9
[PUBMED]    
10. Farshad S, Japoni A, Alborzi A, Hosseini M. Restriction fragment length polymorphism of virulence genes cagA, vacA and ureAB of Helicobacter pylori strains isolated from Iranian patients with gastric ulcer and nonulcer disease. Saudi Med J 2007;28:529-34.  Back to cited text no. 10
[PUBMED]    
11. Chomvarin C, Namwat W, Chaicumpar K, Mairiang P, Sangchan A, Sripa B, et al. Prevalence of Helicobacter pylori vacA, cagA, cagE, iceA and babA2 genotypes in Thai dyspeptic patients. Int J Infect Dis 2008;12:30-6.  Back to cited text no. 11
[PUBMED]    
12. Paniagua GL, Monroy E, Rodríguez R, Arroniz S, Rodríguez C, Cortés JL, et al. Frequency of vacA, cagA and babA2 virulence markers in Helicobacter pylori strains isolated from Mexican patients with chronic gastritis. Ann Clin Microbiol Antimicrob 2009;8:14.  Back to cited text no. 12
    
13. Sheu SM, Hung KH, Sheu BS, Yang HB, Wu JJ. Association of nonsynonymous substitutions in the intermediate region of the vacA gene of Helicobacter pylori with gastric diseases in Taiwan. J Clin Microbiol 2009;47:249-51.  Back to cited text no. 13
[PUBMED]    
14. Sugimoto M, Yamaoka Y. The association of vacA genotype and Helicobacter pylori-related disease in Latin American and African populations. Clin Microbiol Infect 2009;15:835-42.  Back to cited text no. 14
[PUBMED]    
15. Ahmad T, Sohail K, Rizwan M, Mukhtar M, Bilal R, Khanum A. Prevalence of Helicobacter pylori pathogenicity-associated cagA and vacA genotypes among Pakistani dyspeptic patients. FEMS Immunol Med Microbiol 2009;55:34-8.  Back to cited text no. 15
[PUBMED]    
16. Yakoob J, Abid S, Abbas Z, Jafri W, Ahmad Z, Ahmed R, et al. Distribution of Helicobacter pylori virulence markers in patients with gastroduodenal diseases in Pakistan. BMC Gastroenterol 2009;9:87.  Back to cited text no. 16
[PUBMED]    
17. Atherton JC, Cao P, Peek RM Jr, Tummuru MK, Blaser MJ, Cover TL. Mosaicism in vacuolating cytotoxin alleles of Helicobacter pylori. Association of specific vacA types with cytotoxin production and peptic ulceration. J Biol Chem 1995;270:17771-7.  Back to cited text no. 17
[PUBMED]    
18. Sicinschi LA, Correa P, Peek RM Jr, Camargo MC, Delgado A, Piazuelo MB, et al. Helicobacter pylori genotyping and sequencing using paraffin-embedded biopsies from residents of colombian areas with contrasting gastric cancer risks. Helicobacter 2008;13:135-45.  Back to cited text no. 18
[PUBMED]    
19. Hussein NR, Mohammadi M, Talebkhan Y, Doraghi M, Letley DP, Muhammad MK, et al. Differences in virulence markers between Helicobacter pylori strains from Iraq and those from Iran: Potential importance of regional differences in H. pylori-associated disease. J Clin Microbiol 2008;46:1774-9.  Back to cited text no. 19
    
20. Rhead JL, Letley DP, Mohammadi M, Hussein N, Mohagheghi MA, Eshagh Hosseini M, et al. A new Helicobacter pylori vacuolating cytotoxin determinant, the intermediate region, is associated with gastric cancer. Gastroenterology 2007;133:926-36.  Back to cited text no. 20
[PUBMED]    
21. Maciorkowska E, Roszko I, Kowalczuk O, Kaczmarski M, Chyczewski L, Kemona A. The evaluation of vacA gene allele's frequency in Helicobacter pylori strains in children and adults in Podlaskie region. Folia Histochem Cytobiol 2007;45:215-9.  Back to cited text no. 21
[PUBMED]    
22. Mohammadi M, Oghalaie A, Mohajerani N, Massarrat S, Nasiri M, Bennedsen M, et al. Prevalence of Helicobacter pylori vacuolating cytotoxin and its allelic mosaicism as a predictive marker for Iranian dyspeptic patients. Bull Soc Pathol Exot 2003;96:3-5.  Back to cited text no. 22
[PUBMED]    
23. Sgouras DN, Panayotopoulou EG, Papadakos K, Martinez-Gonzalez B, Roumbani A, Panayiotou J, et al. This CagA and VacA polymorphisms do not correlate with severity of histopathological lesions in Helicobacter pylori-infected Greek children. J Clin Microbiol 2009;47:2426-34.  Back to cited text no. 23
[PUBMED]    
24. Lang L, García F. Comparison of E-test and disk diffusion assay to evaluate resistance of Helicobacter pylori isolates to amoxicillin, clarithromycin, metronidazole and tetracycline in Costa Rica. Int J Antimicrob Agents 2004;24:572-7.  Back to cited text no. 24
    
25. Hua J, Ng HC, Yeoh KG, Ho B. Predominance of a single strain of Helicobacter pylori in gastric antrum. Helicobacter 1999;4:28-32.  Back to cited text no. 25
[PUBMED]    
26. van Doorn LJ, Figueiredo C, Sanna R, Plaisier A, Schneeberger P, de Boer W, et al. Clinical relevance of the cagA, vacA, and iceA status of Helicobacter pylori. Gastroenterology 1998;115:58-66.  Back to cited text no. 26
[PUBMED]    
27. Strobel S, Bereswill S, Balig P, Allgaier P, Sonntag HG, Kist M. Identification and analysis of a new vacA genotype variant of Helicobacter pylori in different patient groups in Germany. J Clin Microbiol 1998;36:1285-9.  Back to cited text no. 27
[PUBMED]    
28. Erzin Y, Koksal V, Altun S, Dobrucali A, Aslan M, Erdamar S, et al. Prevalence of Helicobacter pylori vacA, cagA, cagE, iceA, babA2 genotypes and correlation with clinical outcome in Turkish patients with dyspepsia. Helicobacter 2006;11:574-80.  Back to cited text no. 28
[PUBMED]    
29. Sugimoto M, Zali MR, Yamaoka Y. The association of vacA genotypes and Helicobacter pylori-related gastroduodenal diseases in the Middle East. Eur J Clin Microbiol Infect Dis 2009;28:1227-36.  Back to cited text no. 29
    
30. Salehi Z, Abadi AS, Ismail PB, Kqueen CY, Jelodar MH, Kamalidehghan B. Evaluation of Helicobacter pylori vacA genotypes in Iranian patients with peptic ulcer disease. Dig Dis Sci 2009;54:2399-403.  Back to cited text no. 30
[PUBMED]    
31. Jafari F, Shokrzadeh L, Dabiri H, Baghaei K, Yamaoka Y, Zojaji H, et al. vacA genotypes of Helicobacter pylori in relation to cagA status and clinical outcomes in Iranian populations. Jpn J Infect Dis 2008;61:290-3.  Back to cited text no. 31
[PUBMED]    
32. Dabiri H, Maleknejad P, Yamaoka Y, Feizabadi MM, Jafari F, Rezadehbashi M, et al. Distribution of Helicobacter pylori cagA, cagE, oipA and vacA in different major ethnic groups in Tehran, Iran. J Gastroenterol Hepatol 2009;24:1380-6.  Back to cited text no. 32
[PUBMED]    
33. Molaei M, Foroughi F, Mashayekhi R, Haghazali M, Zojaji H, Jafari F, et al. CagA status and VacA subtypes of Helicobacter pylori in relation to histopathologic findings in Iranian population. Indian J Pathol Microbiol 2010;53:24-7.  Back to cited text no. 33
[PUBMED]  Medknow Journal  
34. Conzzz SA, Takeuchi H, Valerín AL, Con-Wong R, Con-Chin GR, Con-Chin VG, et al. Diversity of Helicobacter pylori cagA and vacA genes in Costa Rica: Its relationship with atrophic gastritis and gastric cancer. Helicobacter 2007;12:547-52.  Back to cited text no. 34
    
35. Linpisarn S, Suwan W, Lertprasertsuk N, Koosirirat C, Steger HF, Prommuangyong K, et al. Helicobacter pylori cagA, vacA and iceA genotypes in northern Thai patients with gastric disease. Southeast Asian J Trop Med Public Health 2007;38:356-62.  Back to cited text no. 35
[PUBMED]    
36. Bindayna KM, Al Mahmeed A. VacA genotypes in Helicobacter pylori strains isolated from patients with and without duodenal ulcer in Bahrain. Indian J Gastroenterol 2009;28:175-9.  Back to cited text no. 36
[PUBMED]    
37. Ohno T, Sugimoto M, Nagashima A, Ogiwara H, Vilaichone RK, Mahachai V, et al. Relationship between Helicobacter pylori hopQ genotype and clinical outcome in Asian and Western populations. J Gastroenterol Hepatol 2009;24:462-8.  Back to cited text no. 37
[PUBMED]    
38. Chiarini A, Calà C, Bonura C, Gullo A, Giuliana G, Peralta S, et al. Prevalence of virulence-associated genotypes of Helicobacter pylori and correlation with severity of gastric pathology in patients from western Sicily, Italy. Eur J Clin Microbiol Infect Dis 2009;28:437-46.  Back to cited text no. 38
    
Advanced Biomedical Research
Volume 2014, January
January 2014
Pages 1-5