The comparison of Staphylococcus aureus types 5 and 8 with respect to methicillin resistance in patients admitted to Al-Zahra Hospital by PCR


1 Department of Microbiology, School of Medicine, Isfahan, Iran

2 Isfahan University of Medical Sciences, Isfahan, Iran


Background: Staphylococcus aureus is a common human pathogen in community- and hospital-acquired infection, and its capsule is involved in pathogenesis. The predominance of 2 capsular polysaccharides types 5 and 8, on the surface of clinical isolates, led to the development of conjugate vaccine (Staph VAX) based on capsular polysacchrides types 5 and 8 conjugated to a carrier protein.
The aim of this study was to determine the prevalence of capsular polysaccharides types 5 and 8 Staphylococcus aureus strains among isolates and their comparison with respect to methicillin resistance.
Materials and Methods: We studied the capsular genotypes of 193 isolates that encompassed both hospital- and community-acquired infection in Al-Zahra Hospital of Isfahan city from 2008 to 2009. Cap5 and 8 genes were detected by PCR method. Methicillin resistance was determined by PCR (mecA) and disk diffusion methods as well.
Result: In this population (193 cases), most of the clinical isolates (73%) expressed capsular polysaccharide type 5 (24%) and 8 (49%), whereas 27% were non-typeable. The prevalence of MRSA in type 8 was 67.9%, whereas MRSA isolates in the capsular genotype 5 were 22.2%.
Conclusion: This study Staphylococcus aureus confirms that the prevalence of capsular polysaccharide types (5 and 8) are predominant, and Staphylococcus aureus type 8 is more resistant to methicillin compared to type 5.


1. Verdier I, Durand G, Bes M, Taylor KL, Lina G, Vandenesch F, et al. Identification of the capsular polysaccharides in Staphylococcus aureus clinical isolates by PCR and agglutination tests. J Clin Microbiol 2007;45:725-9.  Back to cited text no. 1
2. Fattom AI, Horwith G, Fuller S, Propst M, Naso R. Development of StaphVAX, a polysaccharide conjugate vaccine against S. aureus infection: From the lab bench to phase III clinical trials. Vaccine 2004;22:880-7.  Back to cited text no. 2
3. Tzianabos AO, Wang JY, Lee JC. Structural rationale for the modulation of abscess formation by Staphylococcus aureus capsular polysaccharides. Proc Natl Acad Sci U S A 2001;98:9365-70.  Back to cited text no. 3
4. Sompolinsky D, Samra Z, Karakawa WW, Vann WF, Schneerson R, Malik Z. Encapsulation and capsular types in isolates of Staphylococcus aureus from different sources and relationship to phage types. J Clin Microbiol 1985;22:828-34.  Back to cited text no. 4
5. Cocchiaro JL, Gomez MI, Risley A, Solinga R, Sordelli DO, Lee JC. Molecular characterization of the capsule locus from non-typeable Staphylococcus aureus. Mol Microbiol 2006;59:948-60.  Back to cited text no. 5
6. von Eiff C, Taylor KL, Mellmann A, Fattom AI, Friedrich AW, Peters G, et al. Distribution of capsular and surface polysaccharide serotypes of Staphylococcus aureus. Diagn Microbiol Infect Dis 2007;58:297-302.  Back to cited text no. 6
7. Melles DC, Taylor KL, Fattom AI, van Belkum A. Serotyping of Dutch Staphylococcus aureus strains from carriage and infection. FEMS Immunol Med Microbiol 2008;52:287-92.  Back to cited text no. 7
8. Essawi T, Na'was T, Hawwari A, Wadi S, Doudin A, Fattom AI. Molecular, antibiogram and serological typing of Staphylococcus aureus isolates recovered from Al-Makased Hospital in East Jerusalem. Trop Med Int Health 1998;3:576-83.  Back to cited text no. 8
9. Na'was T, Hawwari A, Hendrix E, Hebden J, Edelman R, Martin M, et al. Phenotypic and genotypic characterization of nosocomial Staphylococcus aureus isolates from trauma patients. J Clin Microbiol 1998;36:414-20.  Back to cited text no. 9
10. Sutter DE, Summers AM, Keys CE, Taylor KL, Frasch CE, Braun LE, et al. Capsular serotype of Staphylococcus aureus in the era of community-acquired MRSA. FEMS Immunol Med Microbiol 2011;63:16-24.  Back to cited text no. 10
11. Branger C, Goullet P, Boutonnier A, Fournier JM. Correlation between esterase electrophoretic types and capsular polysaccharide types 5 and 8 among methicillin-susceptible and methicillin-resistant strains of Staphylococcus aureus. J Clin Microbiol 1990;28:150-1.  Back to cited text no. 11
12. Roghmann M, Taylor KL, Gupte A, Zhan M, Johnson JA, Cross A, et al. Epidemiology of capsular and surface polysaccharide in Staphylococcus aureus infections complicated by bacteraemia. J Hosp Infect 2005;59:27-32.  Back to cited text no. 12
13. Shinefield H, Black S, Fattom A, Horwith G, Rasgon S, Ordonez J, et al. Use of a Staphylococcus aureus conjugate vaccine in patients receiving hemodialysis. N Engl J Med 2002;346:491-6.  Back to cited text no. 13
14. Forbes BA, Sahm DF, Weissfeld AS. Bailey and Scott's Diagnostic Microbiology. 12 th ed. USA: Elsevier; 2007. p. 172-213.  Back to cited text no. 14
15. Kumar R, Yadav BR, Dev K, Singh RS. A simple protocol for DNA extraction from Staphylococcus aureus. Protocol Online 2008. Available from: [Last accessed on 18.01.2012].  Back to cited text no. 15
16. Baddour MM, AbuElKheir MM, Fatani AJ. Comparison of mecA polymerase chain reaction with phenotypic methods for the detection of methicillin-resistant Staphylococcus aureus. Curr Microbiol 2007;55:473-9.  Back to cited text no. 16
17. Lattar SM, Tuchscherr LP, Caccuri RL, Centrón D, Becker K, Alonso CA, et al. Capsule expression and genotypic among Staphylococcus aureus isolates from patients with chronic or acute osteomyelitis. Infect Immun 2009;77:1968-75.  Back to cited text no. 17
18. Sakoulas G, Gold HS, Venkataraman L, DeGirolami PC, Eliopoulos GM, Qian Q. Methicillin-resistant Staphylococcus aureus: comparison of susceptibility testing methods and analysis of mecA-positive susceptible strains. J Clin Microbiol 2001;39:3946-51.  Back to cited text no. 18
19. Cekovska Z, Panovski N, Petrovska M. Methicillin-resistant Staphylococcus aureus: comparison of susceptibility test methods with mecA gene analysis for determining oxacillin (methicillin) resistance in our clinical isolates. Bratisl Lek Listy 2005;106:163-7.  Back to cited text no. 19
20. Adaleti R, Nakipoglu Y, Karahan ZC, Tasdemir C, Kaya F. Comparison of polymerase chain reaction and conventional methods in detecting methicillin-resistant Staphylococcus aureus. J Infect Dev Ctries 2008;2:46-50.  Back to cited text no. 20