Effects of different extracts of Eucalyptus camaldulensis on Trichomonas vaginalis parasite in culture medium

Authors
Solmaz Hassani 1
Gholamreza Asghari 1
Hossseinali Yousefi 2
Afsaneh Kazemian 3
Mahmood Rafieiean 3
Hossein Yousofi Darani 2
1 Department of Pharmacognosy, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
2 Department of Parasitology and mycology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Medical plant Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
Abstract
Background: Trichomonas vaginalis is considered one of the main causes of vulvovaginitis in women. Metronidazole with vast side effects is now the drug of choice for treatment of this infection. In an attempt to find an alternative drug, the effect of Eucalyptus camaldulensis on this parasite was shown in previous studies. In this investigation, the effect of different extracts of this plant on T. vaginalis in culture medium has been investigated.
Materials and Methods: Five different extracts including total extract, diethyl ether, chloroform, ethyl acetate, and water fractions were prepared. The extracts were dried using vacuum rotary evaporator and then they were used for in vitro anti-trichomonas experiments.
Results: Crude extract of E. camaldulensis showed 80% growth inhibition (GI) in a concentration of 12.5 mg/ml during 24 h. Diethyl ether extract in a concentration of 25 mg/ml showed 100% GI during 24 h. With ethyl acetate extract, 100% GI was detected with the minimum concentration of 12.5 mg/ml in the first 24 h. Finally, water extract in a concentration of 50 mg/ml showed 80% and 100% GI after 48 and 72 h, respectively.
Conclusion: Ethyl acetate fraction is the extract which showed the highest percentage of GI (100%) with the least concentration (12.5 mg/ml) after 24 and 48 h.

Keywords

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Advanced Biomedical Research
Volume 2013, June
June 2013
Pages 1-4