The effect of aqueous cinnamon extract on the apoptotic process in acute myeloid leukemia HL-60 cells


1 Razi Herbal Medicines Research Center, Lorestan University of Medical sciences, Khorramabad, Iran

2 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

3 Department of anatomy, Mazandaran University Of Medical Sciences, Sari, Iran

4 Department of Anatomy and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran


Background: Acute promyelocytic leukemia (APL) is an acute leukemia diagnosed by translocation of chromosomes 15 and 17 [T (15,17)] and aggregation of neoplastic promyelocytes which are incapable of being converted into mature cells. Today, many tend to use medicinal herbs in studies and clinical applications for treatment of cancers. Cinnamon with scientific name "cinnamomumzelanicum" is a shrub of Laurales order, lauraceae family with cinnamomum genus. It is a medicinal shrub with anti-proliferation effect on tumor cells. This study was conducted to determine the effects of aqueous cinnamon extract on HL-60 cells as a model for APL.
Materials and Methods: In this in vitro experimental study, HL-60 cell line was cultured under the influence of cinnamon extract's concentrations of 0.01, 0.1, 1, and 2 mg/ml in with intervals of 24, 48, and 72 h. Growth inhibition and toxic effects of cinnamon extract were evaluated through tetrazolium salt reduction. The effect of this herb on the cell cycle was studied by flow cytometry. The Hoechst stain was used to detect apoptotic cell nuclei.
Results: Cinnamon extract inhibited the growth of HL-60 cells as correlated with concentration and time. After 72 h of treating HL-60 cells with 0.01 mg/l cinnamon extract, the growth of cells was inhibited by 90.1%. Cinnamon extract stopped the cell cycle in G1 phase and the Hoechst staining verified the apoptotic process in those cells.
Conclusion: Considering the inhibitory property of cinnamon extract, we recommend it as a single drug or besides other medications for treating promyelocytic leukemia.


1. Wang ZY, Chen Z. Acute promyelocytic leukemia: From highly fatal to highly curable. Blood 2008;111:2505-15.  Back to cited text no. 1
2. Miller WH Jr, Schipper HM, Lee JS, Singer J, Waxman S. Mechanisms of action of arsenic trioxide. Cancer Res 2002;62:3893-903.  Back to cited text no. 2
3. Randolph TR. Acute promyelocytic Leukemia (AML-M3)-part 1: Pathophysiology, clinical diagnosis, and differentiation therapy. Clin Lab Sci 2000;13:98-105.  Back to cited text no. 3
4. Mchenzie SL. Text book of hematology. 2nd ed. Bultimore: Williams and Wilkins; 1996. p. 385-7.  Back to cited text no. 4
5. Jackson N, Menon BS, Zarina W, Zawiwi N, Naing NN. Why is acute leukemia more common in males? A possible gender determined risk linked to the ABO blood group genes. Ann Hematol 1999;78:233-6.  Back to cited text no. 5
6. Mrozek K, Marcucci G, Paschka P, Whitman SP, Bloomfield CD. Clinical relevance of mutations and gene-expression changes in adult acute myeloid leukemia with normal cytogenetics: Are we ready for a prognostically prioritized molecular classification? Blood 2007;109:431-48.  Back to cited text no. 6
7. Sirard C, Lapidot T, Vormoor J, Cashman JD, Doedens M, Murdoch B, et al. Normal and leukemic SCID-repopulating cells (SRC) coexist in the bone marrow and peripheral blood from CML patients in chronic phase, whereas leukemic SRC are detected in blast crisis. Blood 1996;87:1539-48.  Back to cited text no. 7
8. Newman DJ, Cragg GM, Sanders KM. Natural Products as sources of new drugs over the period. J Nat Prod 2003;66:1022-37.  Back to cited text no. 8
9. Srivastara V, Negi AS, Kumar JK, Gupta MM, Khanuja SP. Plant based anticancer molecules: A chemical and biological profile of some important leads. Bioorg Med Chem 2005;13:5892-908.  Back to cited text no. 9
10. Hwang JS, Ji HJ, Koo KA, Lee NH, Yeo HK, Cheong SW, et al. AIP1, a water-soluble fraction from Artemisia iwayomogi, suppresses thymocyte apoptosis in vitro and down-regulates the expression of fas gene. Biol Pharm Bull 2005;28:921-4.  Back to cited text no. 10
11. Mirheidar H. Plant knowledge; Application of plants in prevention and remedy of diseases. Tehran: Islamic Culture Press; 2004. p. 323-8.  Back to cited text no. 11
12. Shah AH, AL-Shareef AH, Ageel AM. Qureshi S. Toxicity studies in mice of common spices Cinnamomumzeylanicum bark and piper lonum fruits. Plant Foods Hum Nutr 1998;52:231-9.  Back to cited text no. 12
13. Singh G, Maurya S, DeLampasona MP, Catalan CA. A comparision of chemical, antioxidant and antimicrobial studies of cinnamon leaf and bark volatile oil. Food Chem Toxicol 2007;45:1650-61.  Back to cited text no. 13
14. Anderson RA, Broadburst CL, Polansky MM, Schmidt WF, Khan A, Flanagan VP, et al. Isolation and characterization of polyphenol type- A polymers from cinnamon with Insulin-like biological activity. J Agric Food Chem 2004;52:65-70.  Back to cited text no. 14
15. Khan A, Safdar M, Ali Khan MM, Khattak KN, Anderson RA. Cinnamon improves glucose and lipids of people with type 2 diabetes. Diabetes Care2003;26:3215-8.  Back to cited text no. 15
16. Nir Y, Potasman I, Stermer E, Tabak M, Neeman I. Controlled trial of the effect of cinnamon extract on Helicobacter pylori. Helicobacter 2000;5:94-7.  Back to cited text no. 16
17. Calnan CD. Cinnamon dermatitis from an ointment. Contact Dermatitis 1976;2:167-70.  Back to cited text no. 17
18. Kamath JV, Rana AC, Chowdhury AR. Pro-healing effect of cinnamomumzeylanicum bark. Phytother Res 2003;18:970-2.  Back to cited text no. 18
19. Skidmore-Roth L. Handbook of herbs and natural supplements. 2nd ed. St. Louis: Mosey; 2002. p. 38.  Back to cited text no. 19
20. Adame J, Adame H. Parte 1. In: Adame J, A dame H, editors. Curatives plants of north mexico Parte 1. Monterrey Mexico. Ediciones Castillo. 2000; p. 21-260.  Back to cited text no. 20
21. SchoeneNW, Kelly MA, Polansky MM, Anderson RA. Water-soluble polymeric polyphenols from cinnamon inhibit proliferation and alter cell cycle distribution patterns of hematologic tumor cell lines. Cancer Lett 2005;230:134-40.  Back to cited text no. 21
22. Shariati S. Extraction of hydroalcoholic extract of useful products of herbal drugs with methods of identifying. Isfahan: Mani Publication; 1992. p. 12-3.  Back to cited text no. 22
23. Sundartan SG, Milner JA. Impact of organs compounds in garlic oncanine mammary tumor cells in cultures. Cancer lett 1993;174:85-90.  Back to cited text no. 23
24. Spiridonov NA, Konovalov DA, Arkhipov VV. Cytotoxicity of some Russian ethnomedicinal plants and plant compounds. Phytother Res 2005;19:428-32.  Back to cited text no. 24
25. Middleton E Jr, Kandaswami C, Theoharides TC. The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease and cancer. Pharmacol Rev 2000;52:673-751.  Back to cited text no. 25
26. Valenti J. Herbal Medicine, treatment of diseases using herbs, translated by Ahmad Emami. 1st ed. Isfahan University of Medical Sciences, Isfahan Rah-e-Kamal Publication; 2002.  Back to cited text no. 26
27. Lau CB, Ho CY, Kim CF, Leung KN, Fung KP, Tse TF, et al. Cytotoxic activities of Coriolusversicolor (Yunzhi) extract on human Leukemia and lymphoma cells by induction of apoptosis. Life Sci 2004;75:797-808.  Back to cited text no. 27
28. Drexler HG, Minowada J. History and classification of human leukemia-lymphoma cell lines. Leuk Lymphoma 1998;31:305-16.  Back to cited text no. 28
29. Herberton R, Hancock BW. Hodgkin Lymphoma in adoleocents. Cancer Treat Rev 2005;31:339-60.  Back to cited text no. 29
30. Vazquez E, Lucaya J, Castellote A, Piqueras J, Sainz P, Olive T, et al. Neuroimaging in pediatric leukemia and lymphoma: Differential diagnosis. Radiographics 2002;22:1411-28.  Back to cited text no. 30