Advanced Biomedical Research

Antiviral activity of Quercus persica L.: High efficacy and low toxicity

Authors

1 Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

2 Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord; Isfahan Cardiovascular Research institute, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Drug-resistant strain of Herpes simplex virus type 1 (HSV-I) has increased the interest in the use of natural substances.
Aims: This study was aimed to determine minimum inhibitory concentration of hydroalchoholic extract of a traditionally used herbal plant, Quercus persica L., on HSV-1 replication on baby hamster kidney (BHK) cells.
Setting: The study was conducted in Shahrekord University of Medical Sciences, Iran.
Design: This was an experimental study.
Materials and Methods: BHK cells were grown in monolayer culture with Dulbecco's modified Eagle's medium (DMEM) supplemented with 5% fetal calf serum and plated onto 48-well culture plates. Fifty percent cytotoxic concentration (CC50%) of Q. persica L. on BHK cells was determined. Subsequently, 50% inhibitory concentration (IC50%) of the extract on replication of HSV-1 both in interacellular and exteracellular cases was assessed.
Statistical Analysis: Statistic Probit model was used for statistical analysis. The dose-dependent effect of antiviral activity of the extracts was determined by linear regression.
Results: Q. persica L. had no cytotoxic effect on this cell line. There was significant relationship between the concentration of the extract and cell death (P<0.01). IC50s of Q. persica L. on HSV-1, before and after attachment to BHK cells were 1.02 and 0.257 μg/mL, respectively. There was significant relationship between the concentration of this extract and inhibition of cytopathic effect (CPE) (P<0.05). Antioxidant capacity of the extract was 67.5%.
Conclusions: The hydroalchoholic extract of Q. persica L. is potentially an appropriate and promising anti herpetic herbal medicine.

Keywords

1. Khan MT, Ather A, Thompson KD, Gambari R. Extracts and molecules from medicinal plants against Herpes simplex viruses. Antiviral Res 2005;67:107-19.  Back to cited text no. 1
    
2. Whitley RJ, Roizman B. Herpes simplex virus infections. Lancet 2001;357:1513-8.  Back to cited text no. 2
    
3. Brady RC, Bernstein DI. Treatment of herpes simplex virus infections. Antiviral Res 2004;61:73-81.  Back to cited text no. 3
    
4. Whitley RJ, Levin M, Barton N, Hershey BJ, Davis G, Keeney RE, et al. Infections caused by Herpes simplex in the immunocompromised host: Natural history and topical acyclovir therapy. J Infect Dis 1984;150:323-9.  Back to cited text no. 4
    
5. De Clercq E. Antiviral drugs in current clinical use. J Clin Virol 2004;30:115-33.  Back to cited text no. 5
    
6. Kurokawa M, Ochiai H, Nagasaka K, Neki M, Xu H, Kadota S, et al. Antiviral traditional medicines against herpes simplex virus (HSV-1), poliovirus, and measles virus in vitro and their therapeutic efficacies for HSV-1 infection in mice. Antiviral Res 1993;22:175-88.  Back to cited text no. 6
    
7. Teimouri M, Korori S, Moraghebi F, Matinizadeh M. Comparison antibacterial activity of quercus persica and quercus ilex. Iranian J Pharm Res 2004;3:76-7.  Back to cited text no. 7
    
8. Fattahi M. Study of Zagros oak forests and the most important factor of its destruction. Research Institute of Forest and Rangeland (RIFR). 1994; 135.  Back to cited text no. 8
    
9. Makkar HP. Effects and fate of tannins in ruminant animals, adaptation to tannins, and strategies to overcome detrimental effects of feeding tannin-rich feeds. Small Rum Res 2003;49:241-56.  Back to cited text no. 9
    
10. Makkar HP, Dawra RK, Singh B. Tannin levels in leaves of some oak species at different stages of maturity. J Sci Food Agric 1991;54:513-9.  Back to cited text no. 10
    
11. Bainbridge DA. Quercus, a multi-purpose tree for temperate climates. Intl Tree Crops J 1986;3:291-8.  Back to cited text no. 11
    
12. Goodrum PD. Food of the Indians, acorn bread. Pac Hist 1973;17:77-80.  Back to cited text no. 12
    
13. Bainbridge DA. Use of acorns for food in California: Past, present and future, Symposium on Multiple-use Management of California's Hardwoods. 1986; 12-4.  Back to cited text no. 13
    
14. Nakabayashi T. Tannins of fruits and vegetables. Adsorption of polyphenolics with insoluble polyvinylpyrrolidone (polyclar). Jpn J Food Sci Technol 1972;19:84-90.  Back to cited text no. 14
    
15. Hirst EL, Jones JK, Roudier AJ. Structure of acorn starch. J Chem Soc 1948;104:1779-83.  Back to cited text no. 15
    
16. Ofcarcik RP, Burns EE. Chemical and physical properties of selected acorns. J Food Sci 1971;36:576-8.  Back to cited text no. 16
    
17. Kim JO, Lee MJ. Studies on some physicochemical properties of the acorn starch. Kor J Food Sci Technol 1976;8:230-5.  Back to cited text no. 17
    
18. Saffarzadeh A, Vincze L, Csapó J. Determination of the chemical composition of acorn (Quercus branti), Pistacia atlantica and Pistacia khinjuk seeds as non conventional feedstuffs. Acta Agraria Kaposváriensis 1999;3:59-69.  Back to cited text no. 18
    
19. Kekos D, Macris BJ. Production and characterization of amylase from Calvatia gigantea. Appl Environ Microbiol 1983;45:935-41.  Back to cited text no. 19
    
20. Masude T, Isobe J, Jitoe A, Nakamati N. Antioxidative curcuminoids from rhizomes of Curcuma xanthorrhiza. Phytochemistry 1992;31:3645-7.  Back to cited text no. 20
    
21. Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 2002;10:178-82.  Back to cited text no. 21
    
22. Schanderl SH. Methods in Food Analysis. New York: Academic Press; 1970. p. 709.  Back to cited text no. 22
    
23. Kim DO, Jeong SW, Lee CY: Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem 2003;51:321-6.  Back to cited text no. 23
    
24. Ferrea G, Canessa A, Sampietro F, Crudani M, Romussi G, Bassetti D. In vitro activity of a Combretum micranthum extract against Herpes simplex virus types 1 and 2. Antiviral Res 1993;21:317-25.  Back to cited text no. 24
    
25. Rabindran R, Muthulakshmi P, Ganapathy T, Doraiswamy S. Induction of resistance in rice to rice Tungro Virus using horsegram (Vigna unguiculata Walp. Sub sp. unguiculata) seed sprout extract. Madras Agric J 2003;90:286-8.  Back to cited text no. 25
    
26. Villarreal EC. Current and potential therapies for the treatment of herpes virus infections. Prog Drug Res 2003;60:263-307.  Back to cited text no. 26
    
27. Amoros M, Lurton E, Boustie J, Girre L. Comparison of the antiherpes simplex virus activities of propolis and 3-methyl-But-2 Enyl cafferate. J Nat Prod 1994;57:644-7.  Back to cited text no. 27
    
28. Barakat AB, Shoman SA, Dina N, Omar R, Alfarouk R. Antiviral activity and mode of action of Dianthus caryophyllus L. and Lupinus termes L. Seed extracts against in vitro herpes simplex and hepatitis A viruses infection. J Microbiol Antimicrob 2010;2:23-9.  Back to cited text no. 28
    
29. Muliawan SY, Shamala Devi LS, Hashim O, Yusof R. Inhibitory potential of Quercus lusitanica extract on Dengue virus type 2 replication. Southeast Asian J Trop Med Public Health 2006;37:132-5.  Back to cited text no. 29
    
30. Buzzini P, Arapitsas P, Goretti M, Branda E, Turchetti B, Pinelli P. Antimicrobial and Antiviral Activity of Hydrolysable Tannins. Mini Rev Med Chem 2008;8:1179-87.  Back to cited text no. 30
    
31. Keyaerts E, Vijgen L, Pannecouque C, Van DE, Peumans W, Egberink H, et al. Plant lectins are potent inhibitors of coronaviruses by interfering with two targets in the viral replication cycle. Antiviral Res 2007;75:179-87.  Back to cited text no. 31
    
32. Deig EF, Ehresmann DW, Hatch MT, Riedlinger DJ. Inhibition of herpesvirus eplication by marine algae extracts. Antimicrob Agents Chemother 1974;6:524-5.  Back to cited text no. 32
    
33. Baba M, Pauwels R, Balzarini J, Arnout J, Desmyter J, De Clercq E. Sulfated polysaccharides are potent and selective inhibitors of various enveloped viruses, including herpes simplex virus, cytomegalovirus, vesicular stomatitis virus, and human immunodeficiency virus. Antimicrob Agents Chemother 1988;32:1742-5.  Back to cited text no. 33
    
34. Muliawan Y, Shamala SY, Devi LS, Hashim O, Yusof R. Inhibitory potential of Quercus lusitanica extract on Dengue Virus type 2 replication. Southeast Asian J Trop Med Public Health 2006;37:132-5.  Back to cited text no. 34
    
35. Kiarostami KH. Evaluation of the antibacterial effects of Quercus persica and Quercus castaneifolia in tissue culture and perfect plant. J Sci 1998;11:1- 8.  Back to cited text no. 35
    
36. Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 2002;10:178-82.  Back to cited text no. 36
Advanced Biomedical Research
Volume 2013, March
March 2013
Pages 1-6