Identification and cloning of putative water clarification genes of Moringa peregrina (Forssk.) Fiori in E. coli Xl 1 blue cells

Authors
Reihaneh Ghodsi 1
Hamid Mirmohammad Sadeghi 2
Gholamreza Asghari 3
Sepideh Torabi 1
1 Department of Biotechnology, Azad University of Sciences and Researches, Tehran, Iran
2 Department of Pharmaceutical Biotechnology, Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
3 Department of Pharmacognosy, Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Background: Water purification processes include the use of chemical compounds despite the concern that they may induce diseases. An ecological solution to this dilemma can come from the use of plant seeds for this purpose. Moringa peregrina (Forssk.) Fiori seeds have water clarification ability. Therefore, the aim of this work was to look for certain water clarification genes in M. peregrina.
Materials and Methods: After preparation of M. peregrina callus, mRNA was extracted from these cells. After application of reverse transcriptase, the obtained cDNA (s) were used for PCR amplification of the desired genes using primers based on MO 2.1 gene of Moringa oleifera. DNA amplification products were cloned in E. coli Xl 1 blue cells and DNA sequences were compared with Mo 1,2 gene in M. oleifera.
Results: We obtained 3 PCR products (approximately 200, 300, and 400 bps).
Conclusion: After comparison of the sequences of 300bp band obtained from M. peregrina with Mo 1,2 gene in M. oleifera, it seems that 300bp band is a good candidate to investigate regarding its potential flocculent activity.

Keywords

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Advanced Biomedical Research
Volume 2014, January
January 2014
Pages 1-4