Identification and cloning of putative water clarification genes of Moringa peregrina (Forssk.) Fiori in E. coli Xl 1 blue cells

Ghodsi, Reihaneh; Sadeghi, Hamid Mirmohammad; Asghari, Gholamreza; Torabi, Sepideh

Identification and cloning of putative water clarification genes of Moringa peregrina (Forssk.) Fiori in E. coli Xl 1 blue cells

Authors

¹ Department of Biotechnology, Azad University of Sciences and Researches, Tehran, Iran

² Department of Pharmaceutical Biotechnology, Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

³ Department of Pharmacognosy, Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Water purification processes include the use of chemical compounds despite the concern that they may induce diseases. An ecological solution to this dilemma can come from the use of plant seeds for this purpose. Moringa peregrina (Forssk.) Fiori seeds have water clarification ability. Therefore, the aim of this work was to look for certain water clarification genes in M. peregrina.
Materials and Methods: After preparation of M. peregrina callus, mRNA was extracted from these cells. After application of reverse transcriptase, the obtained cDNA (s) were used for PCR amplification of the desired genes using primers based on MO _2.1 gene of Moringa oleifera. DNA amplification products were cloned in E. coli Xl ₁ blue cells and DNA sequences were compared with Mo _1,2 gene in M. oleifera.
Results: We obtained 3 PCR products (approximately 200, 300, and 400 bps).
Conclusion: After comparison of the sequences of 300bp band obtained from M. peregrina with Mo _1,2 gene in M. oleifera, it seems that 300bp band is a good candidate to investigate regarding its potential flocculent activity.

Keywords

References

1.	Jahn SA. Drinking water from Chinese rivers: Challenges of clarification. J Water Supply Res Technol 2001;50:15-27.
2.	Tauscher B. Water treatment by flocculants compounds of higher plants. Plant Res Dev 1994;40:56-70.
3.	Duan J, Gregory J. Coagulation by hydolysing metal salts, Adv. Coloidal Interface Sci 2003;100:475-502.
4.	Flaten TP. Aluminium as a risk factor in Alzheimer's disease, with emphasis on drinking water. Brain Res Bull 2001;55:187-96. [PUBMED]
5.	Martyn CN, Barker DJ, Osmond C, Harris EC, Edwardson JA, Lacey RF. Geographical relation between Alzheimer's disease and aluminum in drinking water. Lancet 1989;1:59-62. [PUBMED]
6.	Ghahreman A. Flora of Iran. Tehran: Institute of Forests and Rangelands Publication; 2001. p. 257.
7.	Abdulkarim SM, Long K, Lai OM, Muhammad SK, Ghazali HM. Some physicochemical properties of Moringa oleifera seed oil extracted using solvent and aqueous enzymatic methods. Food Chem 2005;93:253-63.
8.	Bina B, Shahsavani A, Asghari G, Hassanzadeh A. Comparison of water turbidity removal efficiencies of Moringa oleifera seed extract and poly-aluminum chloride, Water Waste J 2007;61:24-33.
9.	Fahey JW. Moringa oleifera: A review of the medical evidence for its nutritional, therapeutic, and prophylactic properties. Part 1. Trees Life J 2005;1:5-9.
10.	Somali MA, Bajnedi MA, Al-Fhaimani SS. Chemical composition and characteristics of Moringa peregrina seeds and seed oil. J Am Oil Chem Soc 1984;61:85-6.
11.	Okuda T, Bass AU, Nishijima W, Okada M. Isolation and characterization of coagulant extracted from Moringa oleifera seed by salt solution. Water Res 2001;35:405-10.
12.	Ndabigengesere A, Narasiah KS, Talbot BG. Active agents and mechanism of coagulation of turbid waters using Moringa oleifera. Water Res 1995;29:703-10.
13.	Broin M, Santaella C, Cuine S, Kokou K, Peltier G, Joet T. Flocculent activity of a recombinant protein from Moringa oleifera Lam. Seeds. Appl Microbial Biotechnol 2002;60:114-9.
14.	Banejad H, Yazdani V, Rahmani A, Mohajeri S, Olyaie E. Possibility of using Moringa peregrina seeds compared with alum and poly aluminum chloride in sewage treatment. Iran J Health Environ 2010;3;251-60.
15.	Dehshahri SH, Wink M, Afsharypuor S, Asghari G, Mohagheghzadeh A. Antioxidant activity of methanolic leaf extract of Moringa peregrina (Forssk.) Fiori. J Pharmac Sci 2012;7:111-8.
16.	Dehshahri SH, Afsharypuor S, Asghari G, Mohagheghzadeh A. Determination of volatile glucosinolate degradation products in seed coat, stem and in vitro cultures of Moringa peregrina (Forssk.) Fiori. J Pharmac Sci 2012;7:51-6.
17.	Asghari G, Salimian T. Influnce of fructose, glucose and sucrose on flavonolignans formation in Silybum marianum callus culture. J Med Plants 2008;7:16-24.
18.	Gassenschmidt U, Jany KD, Tauscher B, Niebergall H. Isolation and charaterization of a flocculating protein from Moringa oleifera Lam. Biochimica et Biophysica Acta 1995;1243:477-81. [PUBMED]
19.	Suarez MJ, Entenza C, Doerries E, Meyer L, Bourquin J, Sutherl I, et al. Expression of a plant-derived peptide harboring water-cleaning and antimicrobial activities. Biotechnol Bioeng 2003;81:13-20.

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