Advanced Biomedical Research

Transcriptomic comparison of osteopontin, osteocalcin and core binding factor 1 genes between human adipose derived differentiated osteoblasts and native osteoblasts

Authors

1 Division of Genetics, Department of Anatomical Sciences and Molecular Biology, School of Medicine; Applied Physiology Research Center, Isfahan, Iran

2 Division of Genetics, Department of Anatomical Sciences and Molecular Biology, School of Medicine; Pediatric Inherited Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

3 Division of Anatomy, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan, Iran

Abstract

Background: There are significant limitations in repair of irrecoverable bone defects. Stem-cell therapy is a promising approach for the construction of bone tissue. Mesenchymal stem cells (MSCs) have been introduced as basic tools for bone tissue generation. Through MSCs, adipose-derived stem cells (ADSCs) are more interesting. Since the similarity of native osteoblasts and differentiated osteoblasts from ADSCs in terms of gene expression pattern is unknown, this study was designed to compare gene expression patterns of some genes involved in osteogenesis between human native osteoblasts and adipose-derived differentiated osteoblasts.
Materials and Methods: Realtime qRT-PCR was used for studying the gene expression of osteocalcin, osteopontin, and core binding factor alpha 1 (Cbfa1) in human native osteoblasts and adipose derived osteogenic osteoblasts at days 7, 14, 21, and 28 of differentiation.
Results: This study demonstrated that native osteoblasts and differentiated osteoblasts, cultured in common osteogenic medium, have significant differences in gene expression levels for osteocalcin and osteopontin. Compared to native osteoblasts, these genes are expressed lower in all four groups of differentiated osteoblastic cells. We also found, there is a progressive increase in cbfa1 expression over the differentiation period of ADSCs from day 7 to day 28.
Conclusions: Our findings help for better assessment of adipose-derived differentiated cells as a source for cell-based therapy.

Keywords

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Advanced Biomedical Research
Volume 2012, March
March 2012
Pages 1-7