Advanced Biomedical Research

Isolation and phenotypic identification of non-tuberculous mycobacteria existing in Isfahan different water samples

Authors

Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: In recent decades, because of increasing prevalence of immunocompromised disease, non-tuberculous mycobacteria that have been known already as environmental bacteria presented as an important pathogens. In this study, the prevalence of NTM in Isfahan city water samples was determined bydifferent methods including culture methods with biochemical tests.
Materials and Methods: Eighty-five water samples were collected from different sources in Isfahan, Iran. The mycobacterial isolates were identified by the growth characteristics, pigment production, semi-quantitative catalase test, Tween 80 hydrolysis, arylsulfatase test (3 and 14 days), heat-stable catalase (pH 7, 68°C), pyrazin amidase (4 and 7 days), urease, nitrate reduction test, and colony morphology.
Results: Chlorine concentrations of the samples ranged from 0 to 1.8 mg/l. Twenty-one culturable species including M. fortuitum, 23.8% (5 cases); M.smegmatis, 14.3% (3 cases); M. chelonae like organisms, M. terrae complex, M. gordonae and M. mucogenicum, 9.5% (2 cases for each species); M. avium complex, M.phlei, M. xenopi, M. fallax, and M. flavescenc 4.8% (1 case for each species) were identified.
Conclusion: The results of this study showed the incidence of different species of NTM in this geographical region in Iran. Studies show that the prevalence of immunocompromised disease is increasing in the community and it needs different treatment management strategy; therefore, the results of this study can be useful in this strategy.

Keywords

1. Horsburg CR Jr. Mycobacterium avium complex infection in the acquired immunodeficiency syndrome. N Engl J Med 1991;324:1332-8.   Back to cited text no. 1
    
2. Peters M, Müller C, Rüsch-Gerdes S, Seidel C, Göbel U, Pohle HD, et al. Isolation of atypical Mycobacteria from tap water in hospitals and homes. J Infect 1995;31:39-44.   Back to cited text no. 2
    
3. Covert TC, Rodgers MR, Reyes AL, Stelema GN. Occurrence of non tuberculous mycobacteria in environmental sample. Appl Environ Microbiol 1999;68:3159-61.   Back to cited text no. 3
    
4. Lin C, Xue C, Ting ZC, Huan DB, Zhong ZJ. identification of mycobacterium marinum 65KD heat shock protein gene by polymerase chain reaction analyse from lesion of swimming pool granuloma. Chin Med J 2006;119:43-8.   Back to cited text no. 4
    
5. Torvinen E, Suomalainen S, Lehtola MJ, Miettinen IT, Zacheus O, Paulin L, et al. Mycobacteria in water and loose deposits of drinking water distribution system in Finland. Appl Environ Microbiol 2004;70.4:1973-81.   Back to cited text no. 5
    
6. Groote MA, Huitt G. Infections due to rapidly growing mycobacteria. Clin Infect Dis 2006;42:1756-63.   Back to cited text no. 6
[PUBMED]  [FULLTEXT]  
7. Black WC, Berks SG. Cooling towers a potential environmental source of slow growing mycobacteria species. AIHAJ (Fair fax.Va) 2003;64:238-42.   Back to cited text no. 7
    
8. Kotoch VM. Infections due to non-tuberculous mycobacteria (NTM).Indian J Med Res 2004;120:290-304.   Back to cited text no. 8
    
9. Taylor RH, Falkinham JO 3rd, Norton CD, Lechevallier MW. Chlorine, chloramine, chlorine dioxide, and ozone susceptibility of M.avium. Appl Environ Microbiol 2000;66:1702-5.   Back to cited text no. 9
[PUBMED]  [FULLTEXT]  
10. Standard methods for the examination of water and wastewater 18 th ed. Washington DC: American Public Health Association, American water works association, water environmental federation; 1992. p. 1-21.   Back to cited text no. 10
    
11. DPD: National drinking water standards. 5 th ed. Tehran, Iranian industrial research and standards Organization Publications; 1997 (standard no.1053).   Back to cited text no. 11
    
12. Standard methods for the examination of water and wastewater. 19 th ed. Washington DC: American Water Works Association, Water Environment Federation; 1995.   Back to cited text no. 12
    
13. Miyamoto M, Yamaguchi Y. Disinfectant effects of hot water, ultraviolet light, silver ions and chlorine on strains of Legionella and nontuberculous mycobacteria. Microbios 2000;101:7-13.   Back to cited text no. 13
    
14. Naserpour Farivar T, Sharifi B. Prevalence of non-tuberculousis mycobacteria in southeast of Iran. 16 th European Congress of Clinical Microbiology and Infectious Diseases 2006. (ECCMI).   Back to cited text no. 14
    
15. Le Dantec C, Duguet JP, Montiel A, Dumoutier N, Dubrou S, Vincent V. Chlorine disinfection of atypical mycobacteria isolatedfrom a water distribution system. Environ Microbiol 2000;68:1025-32.   Back to cited text no. 15
    
16. Sebakova H, Kozisek F. Incidence of nontuberculous mycobacteria in four hot watersystems using various types of disinfection. Can J Microbiol 2008;54:891-8.   Back to cited text no. 16
    
17. Covert TC, Rodgers MR, Reyese AI, Stelma G Jr. Occurrence of nontuberculous mycobacteria in environmental samples. Appl Environ Microbiol 1999;65:2492-6.   Back to cited text no. 17
    
18. e Chang CT, Wang LY, Liao CY, Huang SP. Identification of nontuberculous mycobacteria existing in tap water by PCR-restriction fragment length polymorphism. Appl Environ Microbiol 2002;68:3159-61.   Back to cited text no. 18
    
19. Shin JH, Lee EJ, Lee HR, Ryu SM, Kim HR, Chang CL, et al. Prevalence of non-tuberculous mycobacteria in hospital environment. J Hosp Infect 2007;65:143-8.   Back to cited text no. 19
[PUBMED]  [FULLTEXT]  
20. Lavania M, Katoch K, Parashar D, Sharma P, Das R, Chauhan DS, et al. Predominance of Mycobacterium fortuitum-chelonae complex in Ghatampur Field Area, Endemic for Leprosy. Indian J Lepr 2008;80: 323-30.   Back to cited text no. 20
[PUBMED]    
21. Koning B,Amer Ina,Sollich V, Koning W. Intra and interpatient variability of the hsp65 and 16S-23S intergnic gene region in Mycobacterium abscessus strains from patients wilh cystic fibrosis. J Clin Microbiol 2005;43:3500-3.   Back to cited text no. 21
    
22. Schulze-Robbecke R,Weber A, Fischeder R. Comparison of decontamination methods for the isolation of Mycobacteria from drinking water samples. J Microbiol Methods 1991;14:177-83.   Back to cited text no. 22
    
23. Bailey and Scott, Baron. Fine Gold. Diagnostic Microbiology. 8th ed. 1990. (Table 41.6).   Back to cited text no. 23
    
24. Masster RN. Mycobacteriology. U.S.A: 1991.(Table 3.11.4).  Back to cited text no. 24
    
Advanced Biomedical Research
Volume 2012, July
July 2012
Pages 1-5