Advanced Biomedical Research

Department of Infectious Disease and Tropical Medicine, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Authors

1 Department of Pharmacology, School of Pharmacy, Isfahan University of Medical Sciences; Isfahan Pharmaceutical Sciences Research Center, Isfahan, Iran

2 Department of Pharmacognosy, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran

3 Department of Clinical Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

4 Isfahan Pharmaceutical Sciences Research Center, Isfahan, Iran

Abstract

Background: Helichrysum oligocephalum DC. from Asteraceae family is an endemic plant growing wild in Iran. This study was carried out to investigate the effect of H. oligocephalum hydroalcoholic extract (HOHE) on ulcerative colitis (UC) induced by acetic acid (AA) in rats.
Materials and Methods: Rats were grouped (n = 6) and fasted for 24 h before colitis induction. Treatments were started 2 h before the induction of colitis and continued for two consecutive days with different doses of HOHE (100, 200, and 400 mg/kg) orally (p.o.) and intraperitoneally (i.p.). The colon tissue was removed and tissue damages were scored after macroscopic and histopathologic assessments.
Results: Among the examined doses of HOHE, 100 mg/kg was the most effective dose that reduced the extent of UC lesions and resulted in significant alleviation. Weight/length ratio as an index of tissue inflammation and extravasation was also diminished in the treatment group administered HOHE at a dose of 100 mg/kg, and the results showed correlation with macroscopic and histopathologic evaluations. These data suggest that HOHE (100 mg/kg) administered either p.o. or i.p. was effective in diminishing inflammation and ulcer indices in this murine model of acute colitis in a non-dose-related manner.
Conclusions: H. oligocephalum could be considered as a suitable anticolitis alternative; however, further studies are needed to support this hypothesis for clinical setting.

Keywords

1. Klein A, Eliakim R. Non-steroidal anti-Inflammatory drugs and inflammatory bowel disease. Pharmaceuticals 2010;3:1084-92.  Back to cited text no. 1
    
2. Mustafa A, El-Medany A, Hagar HH, El-Medany G. Ginkgo biloba attenuates mucosal damage in a rat model of ulcerative colitis. Pharmacol Res 2006;53:324-30.  Back to cited text no. 2
    
3. Lih-Brody L, Powell SR, Collier KP, Reddy GM, Cerchia R, Kahn E, et al. Increased oxidative stress and decreased antioxidant defenses in mucosa of inflammatory bowel disease. Dig Dis Sci 1996;41: 2078-86.  Back to cited text no. 3
    
4. Kucharzik T, Maaser C, Lugering A, Kagnoff M, Mayer L, Targan S, et al. Recent understanding of IBD pathogenesis: Implications for future therapies. Inflamm Bowel Dis 2006;12:1068-83.  Back to cited text no. 4
    
5. Scaldaferri F, Fiocchi C. Inflammatory bowel disease: Progress and current concepts of etiopathogenesis. J Dig Dis 2007;8:171-8.  Back to cited text no. 5
    
6. Fiocchi C. Inflammatory bowel disease: Etiology and pathogenesis. Gastroenterology 1998;115:182-205.  Back to cited text no. 6
    
7. Kirsner JB. Inflammatory bowel disease. Part II: Clinical and therapeutic aspects. Dis Mon 1991;37:669-746.  Back to cited text no. 7
    
8. Poppers DM, Scherl EJ. Prophylaxis against pneumocystis pneumonia in patients with inflammatory bowel disease: Toward a standard of care. Inflamm Bowel Dis 2008;14:106-13.  Back to cited text no. 8
    
9. Summers RW. Novel and future medical management of inflammatory bowel disease. Surg Clin N Am 2007;87:727-41.  Back to cited text no. 9
    
10. Hay JW, Hay AR. Inflammatory bowel disease: Costs-of-illness. J Clin Gastroenterol 1992;14:309-17.  Back to cited text no. 10
    
11. Bodger K. Cost of illness of Crohns disease. Pharmaeconomics 2002;20:639-52.  Back to cited text no. 11
    
12. Sairam K, Rao CV, Goel RK. Effect of Centella asiatica Linn on physical and chemical factors induced gastric ulceration and secretion. Indian J Exp Biol 2001;39:137-42.  Back to cited text no. 12
    
13. Goel RK, Sairam K. Antiulcer drugs from indigenous sources with emphasis on Musa sapientum, Tamrabhasna, Asparagus racemosus and Zingiber officinale. Indian J Pharmacol 2002;34:100-10.  Back to cited text no. 13
    
14. Sumbul S, Ahmad MA, Mohd A. Role of phenolic compounds in peptic ulcer: An overview. J Pharm Bioallied Sci 2011;3:361-7.   Back to cited text no. 14
    
15. Aiyegoro OA, Okoh AI. Preliminary phytochemical screening and in- vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC. BMC Complement Altern Med 2010;10:21-8.  Back to cited text no. 15
    
16. Mozaffarian VA. Dictionary of Iranian plant names. Tehran: Farhang Moaser Publications; 1996. p. 488.  Back to cited text no. 16
    
17. Hedge IC, Akhani H, Kothe-Heinrich G, Podlech D, Rilke S, Rechinger KH. Flora Iranica. Verlagsanstalt: Akademische Druck-u, 1997.  Back to cited text no. 17
    
18. Gonzalez-Colomaa A, Bailena M, Diazb C, Fragab B, Martinez-Diazc R, Zunigad G, et al. Major components of Spanish cultivated Artemisia absinthium populations: Antifeedant, antiparasitic, and antioxidant effects. Ind Crops Prod 2012;37:401-7.  Back to cited text no. 18
    
19. Vahid Khori V, Nayebpour M. Effect of Artemisia absinthium on electrophysiological properties of isolated heart of rats. Physiol Pharmacol 2007;10:303-12.   Back to cited text no. 19
    
20. Sajjadi SE, Jafari A, Naderian M. Chemical composition of the essential oil of Helichrysum oligocephalum. Chem Nat Comp 2009;45:269-71.  Back to cited text no. 20
    
21. Ghassemi-Dehkordi N. Iranian herbal pharmacopoeia. 1 st ed. Vol 1. Tehran: Iranian Health Ministry Publications; 2002. p. 387- 96.  Back to cited text no. 21
    
22. Mascolo N, Izzo A, Maiello A, Dicalo G, Coposso F. Acetic acid-induced colitis in normal and essential fatty acid deficient rats. J Pharmacol Exp Ther 1995;272:469-75.   Back to cited text no. 22
    
23. Motavallian A, Minaiyan M, Rabbani M, Mahzuni P. Anti-inflammatory effect of ondansetron through 5-ht3 receptors on TNBS-induced colitis in rat. EXCLI J 2012;11:30-44.   Back to cited text no. 23
    
24. Minaiyan M, Ghassemi-Dehkordi N, Mahzouni P, Ansari-Roknabady M. Effect of Matricaria aurea (Loefl.) Shultz-Bip. hydroalcoholic extract on acetic acid- induced acute colitis in rats. Iranian J Basic Med Sci 2011;14:67-74.   Back to cited text no. 24
    
25. Minaiyan M, Ghannadi A, Mahzouni P, Nabi-Meibodi M. Anti-ulcerogenic effect of ginger (rhizome of Zingiber officinale Roscoe) hydroalcoholic extract on acetic acid-induced acute colitis in rats. Res Pharm Sci 2008;3:15-22.   Back to cited text no. 25
    
26. Mustafa A, El-Medany A, Hagar HH, El-Medany G. Ginkgo biloba attenuates mucosal damage in a rat model of ulcerative colitis. Pharmacol Res 2006;53:324-30.  Back to cited text no. 26
    
27. Hartmann RM, Morgan Martins MI, Tieppo J, Fillmann HS, Marroni NP. Effect of Boswellia serrata on antioxidant status in an experimental model of colitis rats induced by acetic acid. Dig Dis Sci 2012;57:2038-44.  Back to cited text no. 27
    
28. Mehrabani D, Ziaei M, Hosseini SV, Ghahramani L, Bananzadeh AM, Ashraf MJ. The effect of Calendula officinalis in therapy of acetic acid induced ulcerative colitis in dog as an animal model. Iran Red Crescent Med J 2011;13:884-90.  Back to cited text no. 28
    
29. Amuthan A, Chogtu B, Bairy KL, Sudhakar, Prakash M. Evaluation of diuretic activity of Amaranthus spinosus Linn. aqueous extract in Wistar rats. J Ethnopharmacol 2012;140:424-7.  Back to cited text no. 29
    
30. Patocka J, Pluca B. Pharmacology and toxicology of absinthe. J Appl Biomed 2003;1:199-205.   Back to cited text no. 30
    
31. Sato Y, Itagaki S, Kurokawa T, Ogura J, Kobayashi M, Hirano T, et al. In vitro and in vivo antioxidant properties of chlorogenic acid and caffeic acid. Int J Pharm 2011;403:136-8.   Back to cited text no. 31
    
32. Chagas-Paula DA, Oliveira RB, Da-Silva VC, Gobbo-Neto L, Gasparoto TH, Campanelli AP, et al. Chlorogenic acids from Tithonia diversifolia demonstrate better anti-inflammatory effect than indomethacin and its sesquiterpene lactones. J Ethnopharmacol 2011;136:355-62.   Back to cited text no. 32
    
33. Haeseler G, Maue D, Grosskreutz J, Bufler J, Nentwig B, Piepenbrock S, et al. Voltage-dependent block of neuronal and skeletal muscle sodium channels by thymol and menthol. Eur J Anaesthesiol 2002;19:571-9.  Back to cited text no. 33
    
34. Chauhan PS, Satti NK, Sharma VK, Dutt P, Suri KA, Bani S. Amelioration of inflammatory responses by chlorogenic acid via suppression of pro-inflammatory mediators. J Appl Pharm Sci 2011;1:67-75.  Back to cited text no. 34
    
35. Lima JT, Almeida JR, Barbosa-Filho JM, Assis TS, Silva MS, Dacunha EV, et al. Spasmolytic action of diplotropin, a furanoflavan from Diplotropis ferruginea Benth, involves calcium blockade in ginea-pig ileum. Z Naturforsch B 2005;60:1-8.  Back to cited text no. 35
    
36. Di Carlo G, Autore G, Izzo AA, Maiolino P, Mascolo N, Viola P, et al. Inhibition of intestinal motility and secretion by flavonoids in mice and rats: Structure-activity relationships. J Pharm Pharmacol 1993;45:1054-9.  Back to cited text no. 36
    
37. La Casa C, Villegas I, Alarcon De La Lastra C, Motilva V, Martin Calero MJ. Evidence for protective and antioxidant properties of rutin, a natural flavone, against ethanol induced gastric Lesions. J Ethnopharmacol 2000;71:45-53.  Back to cited text no. 37
    
38. Holma R, Salmenpera P, Riutta A, Virtanen I, Korpela R, Vapaatalo H. Acute effects of the cys-leukotriene-1 receptor antagonist, monteleukast, on experimental colitis in rats. Eur J Pharmacol 2001;429:309-18.  Back to cited text no. 38
    
39. Nijveldt RJ, Van-Nood E, Van-Hoorn DE, Boelens PG, Van-Norren K, Van-Leeuwen PA. Flavonoids: A review of probable mechanisms of action and potential applications. Am J Clin Nutr 2001;74:418-25.   Back to cited text no. 39
    
40. Rao YK, Fang SH, Tzeng YM. Inhibitory effects of the flavonoids isolated from Waltheria indica on the production of NO, TNF-alpha and IL-12 in activated macrophages. Biol Pharm Bull 2005;28:912-5.   Back to cited text no. 40
    
41. Amzal H, Alaoui K, Tok S, Errachidi A, Charof R, Cherrah Y, et al. Protective effect of saponins from Argania spinosa against free radical-induced oxidative haemolysis. Can J Physiol Pharmacol 2007;85:918-27.  Back to cited text no. 41
    
42. Aguwa CN, Nwako SO. Preliminary studies of the root extracts of Nauclea latifolia smith for anti-ulcer properties. Niger J Pharm Sci 1988;4:16-23.  Back to cited text no. 42
Advanced Biomedical Research
Volume 2014, February
February 2014
Pages 1-7