Advanced Biomedical Research

The study of mutation in 23S rRNA resistance gene of Helicobacter pylori to clarithromycin in patients with gastrointestinal disorders in Isfahan - Iran

Authors

1 Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

2 Department of Gastroenterology, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Helicobacter pylori antimicrobial resistance is an important factor responsible for treatment failure. The purpose of this study was evaluating the prevalence of point mutations in clarithromycin-resistant clinical isolates of H. pylori in Isfahan city of Iran.
Materials and Methods: Thirty isolates of H. pylori from 130 biopsy specimens were isolated by culture and confirmed by biochemical and PCR tests. The MIC of clarithromycin antibiotic for 30 clinical isolates of H. pylori was determined by E-test method. The point mutations in the 288 bp of 23S rRNA gene of H. Pylori were investigated in four clarithromycin-resistant clinical isolates by PCR followed by sequencing.
Results: Among 30 isolates of H. pylori, 4 cases were resistant to clarithromycin. One point mutation was found at position T2243C in the 23S rRNA gene in all resistance isolates.
Conclusions: In our study, H. pylori resistance to clarithromycin associated with point mutation at position 2243 (T2243C).

Keywords

1. Fontana C, Favaro M, Minelli S, Criscuolo AA, Pietroiusti A, Galante A, et al. New site of modification of 23S rRNA associated with clarithromycin resistance of Helicobacter pylori clinical isolates. Antimicrob Agents Chemother 2002;46:3765-9.  Back to cited text no. 1
    
2. Gerrits MM, van Vliet AH, Kuipers EJ, Kusters JG. Helicobacter pylori and antimicrobial resistance: Molecular mechanisms and clinical implications. Lancet Infect Dis 2006;6:699-709.  Back to cited text no. 2
    
3. Jones KR, Cha JH, Merrell DS. Who's winning the war? Molecular mechanisms of antibiotic resistance in Helicobacter pylori. Curr Drug Ther 2008;3:190-203.  Back to cited text no. 3
    
4. Rimbara E, Noguchi N, Kijima H, Yamaguchi T, Kawai T, Sasatsu M. Mutations in the 23S rRNA gene of clarithromycin-resistant Helicobacter pylori from Japan. Int J Antimicrob Agents 2007;30:250-4  Back to cited text no. 4
    
5. Hosseini E, Poursina F, de Wiele TV, Safaei HG, Adibi P. Helicobacter pylori in Iran: A systematic review on the association of genotypes and gastroduodenal diseases. J Res Med Sci 2012;17:280-92.  Back to cited text no. 5
[PUBMED]  Medknow Journal  
6. Suerbaum S, Josenhans C. Helicobacter pylori evolution and phenotypic diversification in a changing host. Nat Rev Microbiol 2007;5:441-52.  Back to cited text no. 6
    
7. Baglan PH, Bozdayi G, Ozkan M, Ahmed K, Bozdayi AM, Ozden A. Clarithromycin resistance prevalence and Icea gene status in Helicobacter pylori clinical isolates in Turkish patients with duodenal ulcer and functional dyspepsia. J Microbiol 2006;44:409-16.  Back to cited text no. 7
    
8. Wang G, Wilson TJ, Jiang Q, Taylor DE. Spontaneous mutations that confer antibiotic resistance in Helicobacter pylori. Antimicrob Agents Chemother 2001;45:727-33.  Back to cited text no. 8
    
9. Agudo S, Pérez-Pérez G, Alarcón T, López-Brea M. High prevalence of clarithromycin-resistant Helicobacter pylori strains and risk factors associated with resistance in Madrid, Spain. J Clin Microbiol 2010;48:3703-7.  Back to cited text no. 9
    
10. Garrido L, Toledo H. Novel genotypes in Helicobacter pylori involving domain V of the 23S rRNA gene. Helicobacter 2007;12:505-9.  Back to cited text no. 10
    
11. Toracchio S, Aceto GM, Mariani‐Costantini R, Battista P, Marzio L. Identification of a novel mutation affecting domain V of the 23S rRNA gene in Helicobacter pylori. Helicobacter 2004;9:396-9.  Back to cited text no. 11
    
12. E-Test Technical Guide 8. Susceptibility testing of Helicobacter pylori. Solna, Sweden: AB Biodisk; 2000.  Back to cited text no. 12
    
13. Kim JM, Kim JS, Kim N, Kim YJ, Kim IY, Chee YJ, et al. Gene mutations of 23S rRNA associated with clarithromycin resistance in Helicobacter pylori strains isolated from Korean patients. J Microbiol Biotechnol 2008;18:1584-9.  Back to cited text no. 13
    
14. Taylor DE, Ge Z, Purych D, Lo T, Hiratsuka K. Cloning and sequence analysis of two copies of a 23S rRNA gene from Helicobacter pylori and association of clarithromycin resistance with 23S rRNA mutations. Antimicrob Agents Chemother 1997;41:2621-8.  Back to cited text no. 14
    
15. Tay CY, Mitchell H, Dong Q, Goh KL, Dawes IW, Lan R. Population structure of Helicobacter pylori among ethnic groups in Malaysia: Recent acquisition of the bacterium by the malay population. BMC Microbiol 2009;9:126.  Back to cited text no. 15
    
16. Yula E, Nagiyev T, Kaya OA, Inci M, Celik MM, Köksal F. Detection of primary clarithromycin resistance of Helicobacter pylori and association between cagA (+) status and clinical outcome. Folia Microbiol (Praha) 2013;58:141-6.  Back to cited text no. 16
    
17. Kadayifci A, Uygun A, Kilciler G, Kantarcioglu M, Kara M, Ozcan A, et al. Low efficacy of clarithromycin including sequential regimens for Helicobacter pylori infection. Helicobacter 2012;17:121-6.  Back to cited text no. 17
    
18. Ho SL, Tan EL, Sam CK, Goh KL. Clarithromycin resistance and point mutations in the 23S rRNA gene in Helicobacter pylori isolates from Malaysia. J Dig Dis 2010;11:101-5.  Back to cited text no. 18
    
19. Li Y, Rimbara E, Thirumurthi S, Trespalacios A, Reddy R, Sabounchi S, et al. Detection of clarithromycin resistance in Helicobacter pylori following noncryogenic storage of rapid urease tests for 30 days. J Dig Dis 2012;13:54-9.  Back to cited text no. 19
    
20. Mirzaei N, Poursina F, Faghri J, Talebi M, Khataminezhad MR, Hasanzadeh A, et al. Prevalence of resistance to Helicobacter pylori strains to selected antibiotics in Isfahan, Iran. Jundishapur J Microbiol 2013;6:e6342.  Back to cited text no. 20
    
21. Hao Q, Li Y, Zhang ZJ, Liu Y, Gao H. New mutation points in 23S rRNA gene associated with Helicobacter pylori resistance to clarithromycin in northeast China. World J Gastroenterol 2004;10:1075-7.  Back to cited text no. 21
    
22. Kargar M, Baghernejad M, Doosti A, Ghorbani-Dalini S. Clarithromycin resistance and 23S rRNA mutations in Helicobacter pylori isolates in Iran. African J Microbiol Res 2011;5:853-6.  Back to cited text no. 22
    
23. Savari M, Abdollahi H, Zahedi MJ, Darvish Moghadam S, Hayatbakhsh Abasi M. Detection of A2142C, A2142G, and A2143G mutations in 23s rRNA gene conferring resistance to clarithromycin among Helicobacter pylori isolates in Kerman, Iran. Iran J Med Sci 2011;36:104-10.  Back to cited text no. 23
    
24. Hirata K, Suzuki H, Nishizawa T, Tsugawa H, Muraoka H, Saito Y, et al. Contribution of efflux pumps to clarithromycin resistance in Helicobacter pylori. J Gastroenterol Hepatol 2010;25 (Suppl 1):S75-9.  Back to cited text no. 24
Advanced Biomedical Research
Volume 2014, March
March 2014
Pages 1-4