Advanced Biomedical Research

Macroscopic effect of blue light cure on wound healing in NMRI mice NMRI

Authors

1 Skin Diseases and Leishmaniasis Research Center, Department of Dermatology, Isfahan University of Medical Sciences, Isfahan; Skin and Stem Cell Research Center, Department of Dermatology, Tehran University of Medical Sciences, Tehran, Iran

2 Department of Radiology and Medical Physics, Tehran University of Medical Sciences, Tehran, Iran

3 Department of Dental Pathology, Islamic Azad University of Khorasgan, Isfahan, Iran

4 Department of Physiology and Biophysics, Islamic Azad University of Tehran, Tehran, Iran

5 Skin and Stem Cell Research Center, Department of Dermatology, Tehran University of Medical Sciences, Tehran; Skin Diseases and Leishmaniasis Research Center, Department of Dermatology, Isfahan University of Medical Sciences, Isfahan, Iran

6 Skin Diseases and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Wound healing is a complex process and has been an ongoing challenge all over the world. Some studies have suggested light cure as a modality to accelerate wound repair. It can induce fibroblast proliferation, increase collagen synthesis and activate cellular processes involved in expression of procollagen type I and III mRNA. This study was designed to assess the macroscopic effect of halogen dental curing blue light on full-thickness open wound healing in NMRI mice.
Materials and Methods: Forty male NMRI mice were divided into control and treatment groups. A full-thickness wound of 6 mm in diameter was induced on the lower back of all mice under general anesthesia and sterile conditions. The mice of the treatment group received a 5-min exposure of halogen light Coltolux II (QHL), 420-500 nm, daily for 7 days. The diameter of the wound was measured in both the treatment and the control groups every second day up to Day 14. Data were analyzed by SPSS version 12 software using Student's t-test. A significance level of P ≤ 0.05 was considered for each comparison.
Results: There was a significant difference in wound diameter between the control and the treatment groups at all measurements after Day 3 (P ≤ 0/05).
Conclusion: The results of this study suggest improvement of full-thickness wound healing by daily irradiation of halogen dental curing blue light of 420-500 nm for 7 days.

Keywords

1. da Silva JP, da Silva MA, Almeida AP, Lombardi Junior I, Matos AP. Laser therapy in the tissue repair process: A literature review. Photomed Laser Surg 2010;28:17-21.  Back to cited text no. 1
    
2. Pinheiro AL. Advances and perspectives on tissue repair and healing.Photomed Laser Surg 2009;27:833-6.  Back to cited text no. 2
    
3. Dazzi F, Horwood NJ. Potential of mesenchymal stem cell therapy. Curr Opin Oncol 2007;19:650-5.  Back to cited text no. 3
    
4. Zhao LL, Davidson JD, Wee SC, Roth SI, Mustoe TA. Effect of hyperbaric oxygen and growth factors on rabbit ear ischemic ulcers. Arch Surg 1994;129:1043-9.  Back to cited text no. 4
    
5. Bouachour G, Cronier P, Gouello JP, Toulemonde JL, Talha A, Alquier P. Hyperbaric oxygen therapy in the management of crush injuries: A randomized double-blind placebo-controlled clinical trial. J Trauma 1996;41:333-9.  Back to cited text no. 5
    
6. Meirelles GC, Santos JN, Chagas PO, Moura AP, Pinheiro AL. A comparative study of the effects of laser photobiomodulation on the healing of third-degree burns: A histological study in rats. Photomed Laser Surg 2008;26:159-66.  Back to cited text no. 6
    
7. Pinheiro AL, Meireles GC, Carvalho CM, de Barros Vieira AL, dos Santos JN, Ramalho LM. Biomodulative effects of polarized light on the healing of cutaneous wounds on nourished and undernourished wistar rats. Photomed Laser Surg 2006;24:616-24.  Back to cited text no. 7
    
8. Whelan HT, Buchmann EV, Dhokalia A, Kane MP, Whelan NT, Wong-Riley MT, et al. Effect of NASA light-emitting diode irradiation on molecular changes for wound healing in diabetic mice. J Clin Laser Med Surg 2003;21:67-74.  Back to cited text no. 8
    
9. Whinfield AL, Aitkenhead I. The light revival: Does phototherapy promote wound healing? A review. Foot (Edinb) 2009;19:117-24.  Back to cited text no. 9
    
10. Vasheghani MM, Bayat M, Rezaei F, Bayat A, Karimipour M. Effect of low-level laser therapy on mast cells in second-degree burns in rats. Photomed Laser Surg 2008;26:1-5.  Back to cited text no. 10
    
11. Enwemeka CS, Williams D, Hollosi S, Yens D, Enwemeka SK. Visible 405 nm SLD light photo-destroys methicillin-resistant Staphylococcus aureus (MRSA) in vitro. Lasers Surg Med 2008;40:734-7.  Back to cited text no. 11
    
12. Woodruff LD, Bounkeo JM, Brannon WM, Dawes KS, Barham CD, Waddell DL, et al. The efficacy of laser therapy in wound repair: A meta-analysis of the literature. Photomed Laser Surg 2004;22:241-7.  Back to cited text no. 12
    
13. Enwemeka CS, Parker JC, Dowdy DS, Harkness EE, Sanford LE, Woodruff LD. The efficacy of low-power lasers in tissue repair and pain control: A meta analysis study. Photomed Laser Surg 2004;22:323-9.  Back to cited text no. 13
    
14. Demidova-Rice TN, Salomatina EV, Yaroslavsky AN, Herman IM, Hamblin MR. Low-level light stimulates excisional wound healing in mice. Lasers Surg 2007;39:706-15.  Back to cited text no. 14
    
15. Enwemeka CS, Williams D, Enwemeka SK, Hollosi S, Yens D. Blue 470-nm light kills methicillin-resistant staphylococcus aureus (MRSA) in vitro.Photomed Laser Surg 2009;27:221-6.  Back to cited text no. 15
    
16. Maclean M, MacGregor SJ, Anderson JG, Woolsey G. High-intensity narrow-spectrum light inactivation and wavelength sensitivity of staphylococcus aureus. FEMS Microbiol Lett 2008;285:227-32.  Back to cited text no. 16
    
17. Ankri R, FriedmanH, Savion N, Kotev-Emeth S, Breitbart H, Lubart R.Visible light induces (NO) formation in sperm and endothelial cells. Laser Surg Med 2010;42:348-52.  Back to cited text no. 17
Advanced Biomedical Research
Volume 2014, March
March 2014
Pages 1-3