Platelet-rich plasma application in chondrogenesis


1 Department of Anatomical Sciences, Paramedical School, Guilan University of Medical Sciences, Langeroud, Iran

2 Department of Anatomical Sciences and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran

3 Department of Biology, Molecular and Developmental Division, Faculty of Sciences, University of Isfahan, Isfahan, Iran

4 Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran


Platelet-rich plasma (PRP), an autologous derivative of whole blood, has been recently used in surgical treatment. PRP contains growth factors including transforming growth factor-β (TGF-β), insulin-like growth factor (IGF), platelet-derived growth factor (PDGF), basic fibroblast growth factor (bFGF), and vascular endothelial growth factor (VEGF) and also bioactive proteins that influence the healing of tendon, ligament, muscle, and bone. This article describes the current clinical applications of PRP in chondrogenesis. This study reviews and evaluates the studies that have been published in the field of chondrogenesis. All aspects of using PRP in chondrogenesis are reviewed.


1. Arora NS, Ramanayake T, Ren YF, Romanos GE. Platelet-rich plasma: A literature review. Implant Dent 2009;18:303-10.  Back to cited text no. 1
2. Mehta S, Watson JT. Platelet rich concentrate: Basic science and current clinical applications. J Orthop Trauma 2008;22:432-8.  Back to cited text no. 2
3. Marx RE. Platelet-rich plasma (PRP): What is PRP and what is not PRP? Implant Dent 2001;10:225-8.  Back to cited text no. 3
4. Anitua E, Sanchez M, Orive G, Andia I. The potential impact of the preparation rich in growth factors (PRGF) in different medical fields. Biomaterials 2007;28:4551-60.  Back to cited text no. 4
5. Leitner GC, Gruber R, Neumuller J, Wagner A, Kloimstein P, Hocker P, et al. Platelet content and growth factor release in platelet-rich plasma: A comparison of four different systems. Vox Sang 2006;91:135-9.  Back to cited text no. 5
6. Froum SJ, Wallace SS, Tarnow DP, Cho SC. Effect of platelet-rich plasma on bone growth and osseointegration in human maxillary sinus grafts: Three bilateral case reports. Int J Periodont Restorat Dent 2002;22:45-53.  Back to cited text no. 6
7. Petrungaro PS. Using platelet-rich plasma to accelerate soft tissue maturation in esthetic periodontal surgery. Compend Contin Educ Dent 2001;22:729-32,34,36.  Back to cited text no. 7
8. Lopez-Vidriero E, Goulding KA, Simon DA, Sanchez M, Johnson DH. The use of platelet-rich plasma in arthroscopy and sports medicine: Optimizing the healing environment. Arthroscopy 2010;26:269-78.  Back to cited text no. 8
9. Zhai W, Wang N, Qi Z, Gao Q, Yi L. Platelet-rich plasma reverses the inhibition of tenocytes and osteoblasts in tendon-bone healing. Orthopedics 2012;35:e520-5.  Back to cited text no. 9
10. Han J, Meng H, Tang J, Li S, Tang Y, Chen Z. The effect of different platelet-rich plasma concentrations on proliferation and differentiation of human periodontal ligament cells in vitro. Cell Prolif 2007;40:241-52.  Back to cited text no. 10
11. Awad HA, Halvorsen YD, Gimble JM, Guilak F. Effects of transforming growth factor beta1 and dexamethasone on the growth and chondrogenic differentiation of adipose-derived stromal cells. Tissue Eng 2003;9:1301-12.  Back to cited text no. 11
12. Ansar MM, Esfandiariy E, Mardani M, Hashemibeni B, Zarkesh-Esfahani SH, Hatef M, et al. A comparative study of aggrecan synthesis between natural articular chondrocytes and differentiated chondrocytes from adipose derived stem cells in 3D culture. Adv Biomed Res. 2012;1:24.  Back to cited text no. 12
13. Sheyn D, Pelled G, Zilberman Y, Talasazan F, Frank JM, Gazit D, et al. Nonvirally engineered porcine adipose tissue-derived stem cells: Use in posterior spinal fusion. Stem Cells 2008;26:1056-64.  Back to cited text no. 13
14. Nikolidakis D, Jansen JA. The biology of platelet-rich plasma and its application in oral surgery: Literature review. Tissue Eng Part B Rev 2008;14:249-58.  Back to cited text no. 14
15. Fortier LA, Barker JU, Strauss EJ, McCarrel TM, Cole BJ. The role of growth factors in cartilage repair. Clin Orthop Relat Res 2011;469:2706-15.  Back to cited text no. 15
16. Mishra A, Tummala P, King A, Lee B, Kraus M, Tse V, et al. Buffered platelet-rich plasma enhances mesenchymal stem cell proliferation and chondrogenic differentiation. Tissue Eng Part C Methods 2009;15:431-5.  Back to cited text no. 16
17. Sun Y, Feng Y, Zhang CQ, Chen SB, Cheng XG. The regenerative effect of platelet-rich plasma on healing in large osteochondral defects. Int Orthop 2010;34:589-97.  Back to cited text no. 17
18. Wrotniak M, Bielecki T, Gazdzik TS. Current opinion about using the platelet-rich gel in orthopaedics and trauma surgery. OrtopTraumatol Rehabil 2007;9:227-38.  Back to cited text no. 18
19. Grimaud E, Heymann D, Redini F. Recent advances in TGF-beta effects on chondrocyte metabolism. Potential therapeutic roles of TGF-beta in cartilage disorders. Cytokine Growth Factor Rev 2002;13:241-57.  Back to cited text no. 19
20. Fan H, Hu Y, Qin L, Li X, Wu H, Lv R. Porous gelatin-chondroitin-hyaluronate tri-copolymer scaffold containing microspheres loaded with TGF-beta1 induces differentiation of mesenchymal stem cells in vivo for enhancing cartilage repair. J Biomed Mater Res Part A 2006;77:785-94.  Back to cited text no. 20
21. Pufe T, Harde V, Petersen W, Goldring MB, Tillmann B, Mentlein R. Vascular endothelial growth factor (VEGF) induces matrix metalloproteinase expression in immortalized chondrocytes. J Pathol 2004;202:367-74.  Back to cited text no. 21
22. Ferrara N, Gerber HP, LeCouter J. The biology of VEGF and its receptors. Nat Med 2003;9:669-76.  Back to cited text no. 22
23. Ataliotis P. Platelet-derived growth factor A modulates limb chondrogenesis both in vivo and in vitro. Mech Dev 2000;94:13-24.  Back to cited text no. 23
24. Solchaga LA, Penick K, Goldberg VM, Caplan AI, Welter JF. Fibroblast growth factor-2 enhances proliferation and delays loss of chondrogenic potential in human adult bone-marrow-derived mesenchymal stem cells. Tissue engineering Part A 2010;16:1009-19.  Back to cited text no. 24
25. Kabiri A, Esfandiari E, Hashemibeni B, Kazemi M, Mardani M, Esmaeili A. Effects of FGF-2 on human adipose tissue derived adult stem cells morphology and chondrogenesis enhancement in transwell culture. Biochem Biophys Res Commun 2012;424:234-8.  Back to cited text no. 25
26. Yokota M, Tatsumi N, Tsuda I, Nishioka T, Takubo T. CTAD as a universal anticoagulant. J Anal Methods Chem 1900;25:17-20.  Back to cited text no. 26
27. Dohan Ehrenfest DM, Rasmusson L, Albrektsson T. Classification of platelet concentrates: From pure platelet-rich plasma to leucocyte- and platelet-rich fibrin. Trends Biotechol 2009;27:158-67.  Back to cited text no. 27
28. Burmeister SL, Hartwig D, Limb GA, Kremling C, Hoerauf H, Muller M, et al. Effect of various platelet preparations on retinal muller cells. Invest Ophthalmol Vis Sci 2009;50:4881-6.  Back to cited text no. 28
29. Akeda K, An HS, Okuma M, Attawia M, Miyamoto K, Thonar EJ, et al. Platelet-rich plasma stimulates porcine articular chondrocyte proliferation and matrix biosynthesis. Osteoarthritis Cartilage 2006;14:1272-80.  Back to cited text no. 29
30. Saito M, Takahashi KA, Arai Y, Inoue A, Sakao K, Tonomura H, et al. Intraarticular administration of platelet-rich plasma with biodegradable gelatin hydrogel microspheres prevents osteoarthritis progression in the rabbit knee. Clin Exp Rheumatol 2009;27:201-7.  Back to cited text no. 30
31. Zhu J, Cai B, Ma Q, Chen F, Wu W. Cell bricks-enriched platelet-rich plasma gel for injectable cartilage engineering-an in vivo experiment in nude mice. J Tissue Eng Regen Med 2012 [In Press].  Back to cited text no. 31
32. Spreafico A, Chellini F, Frediani B, Bernardini G, Niccolini S, Serchi T, et al. Biochemical investigation of the effects of human platelet releasates on human articular chondrocytes. J Cell Biochem 2009;108:1153-65.  Back to cited text no. 32
33. Lee HR, Park KM, Joung YK, Park KD, Do SH. Platelet-rich plasma loaded hydrogel scaffold enhances chondrogenic differentiation and maturation with up-regulation of CB1 and CB2. J Control Release 2012;159:332-7.  Back to cited text no. 33
34. Kaps C, Loch A, Haisch A, Smolian H, Burmester GR, Haupl T, et al. Human platelet supernatant promotes proliferation but not differentiation of articular chondrocytes. Med Biol Eng Comput 2002;40:485-90.  Back to cited text no. 34
35. Choi YC, Morris GM, Sokoloff L. Effect of platelet lysate on growth and sulfated glycosaminoglycan synthesis in articular chondrocyte cultures. Arthritis Rheum 1980;23:220-4.  Back to cited text no. 35
36. Drengk A, Zapf A, Stürmer EK, Stürmer KM, Frosch KH. Influence of platelet-rich plasma on chondrogenic differentiation and proliferation of chondrocytes and mesenchymal stem cells. Cells Tissues Organs 2009;189:317-26.  Back to cited text no. 36
37. Park SI, Lee HR, Kim S, Ahn MW, Do SH. Time-sequential modulation in expression of growth factors from platelet-rich plasma (PRP) on the chondrocyte cultures. Mol Cell Biochem 2012;361:9-17.  Back to cited text no. 37
38. Wu W, Chen F, Liu Y, Ma Q, Mao T. Autologous injectable tissue-engineered cartilage by using platelet-rich plasma: Experimental study in a rabbit model. J Oral Maxillofac Surg 2007;65:1951-7.  Back to cited text no. 38
39. Wu W, Zhang J, Dong Q, Liu Y, Mao T, Chen F. Platelet-rich plasma-A promising cell carrier for micro-invasive articular cartilage repair. Med Hypotheses 2009;72:455-7.  Back to cited text no. 39
40. Dhinsa BS, Adesida AB. Current clinical therapies for cartilage repair, their limitation and the role of stem cells. Curr Stem Cell Res Ther 2012;7:143-8.  Back to cited text no. 40
41. Mehlhorn AT, Niemeyer P, Kaschte K, Muller L, Finkenzeller G, Hartl D, et al. Differential effects of BMP-2 and TGF-beta1 on chondrogenic differentiation of adipose derived stem cells. Cell Prolif 2007;40:809-23.  Back to cited text no. 41
42. Hashemibeni B, Goharian V, Esfandiari E, Sadeghi F, Fasihi F, Alipur R, et al. An animal model study for repair of tracheal defects with autologous stem cells and differentiated chondrocytes from adipose-derived stem cells. J Pediatr Surg 2012;47:1997-2003.  Back to cited text no. 42
43. Cheng NC, Estes BT, Awad HA, Guilak F. Chondrogenic differentiation of adipose-derived adult stem cells by a porous scaffold derived from native articular cartilage extracellular matrix. Tissue Eng Part A 2009;15:231-41.  Back to cited text no. 43
44. Weibrich G, Kleis WK, Hafner G, Hitzler WE. Growth factor levels in platelet-rich plasma and correlations with donor age, sex, and platelet count. J Craniomaxillofac Surg 2002;30:97-102.  Back to cited text no. 44
45. Mifune Y, Matsumoto T, Takayama K, Ota S, Li H, Meszaros LB, et al. The effect of platelet-rich plasma on the regenerative therapy of muscle derived stem cells for articular cartilage repair. Osteoarthritis Cartilage 2013;21:175-85.  Back to cited text no. 45
46. Kruger JP, Hondke S, Endres M, Pruss A, Siclari A, Kaps C. Human platelet-rich plasma stimulates migration and chondrogenic differentiation of human subchondral progenitor cells. J Orthop Res 2012;30:845-52.  Back to cited text no. 46
47. Feng X, Tian S, Sun K, Zhang J, Zhang C, Liu S, et al. Effect of platelet lysate on chondrogenic differentiation of human umbilical cord derived mesenchymal stem cells in vitro. Zhongguo xiu fu chong jian wai ke za zhi 2011;25:1250-5.  Back to cited text no. 47
48. Xie X, Wang Y, Zhao C, Guo S, Liu S, Jia W, et al. Comparative evaluation of MSCs from bone marrow and adipose tissue seeded in PRP-derived scaffold for cartilage regeneration. Biomaterials 2012;33:7008-18.  Back to cited text no. 48
49. Lee HR, Park KM, Joung YK, Park KD, Do SH. Platelet-rich plasma loaded in situ-formed hydrogel enhances hyaline cartilage regeneration by CB1 upregulation. J Biomed Mater Res Part A 2012;100:3099-107.  Back to cited text no. 49
50. Wu CC, Chen WH, Zao B, Lai PL, Lin TC, Lo HY, et al. Regenerative potentials of platelet-rich plasma enhanced by collagen in retrieving pro-inflammatory cytokine-inhibited chondrogenesis. Biomaterials 2011;32:5847-54.  Back to cited text no. 50
51. Bendinelli P, Matteucci E, Dogliotti G, Corsi MM, Banfi G, Maroni P, et al. Molecular basis of anti-inflammatory action of platelet-rich plasma on human chondrocytes: Mechanisms of NF-kappa B inhibition via HGF. J Cell Physiol 2010;225:757-66.  Back to cited text no. 51
52. van Buul GM, Koevoet WL, Kops N, Bos PK, Verhaar JA, Weinans H, et al. Platelet-rich plasma releasate inhibits inflammatory processes in osteoarthritic chondrocytes. Am J Sports Med 2011;39:2362-70.  Back to cited text no. 52
53. Pereira RC, Scaranari M, Benelli R, Strada P, Reis RL, Cancedda R, et al. Dual effect of platelet lysate on human articular cartilage: A maintenance of chondrogenic potential and a transient pro-inflammatory activity followed by an inflammation resolution. Tissue Eng Part A 2013 [In Press].  Back to cited text no. 53