The concentration of aflatoxin M 1 in the mothers' milk in Khorrambid City, Fars, Iran


1 Department of Medical Mycology and Parasitology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

2 Department of Medical Mycology and Parasitology, Faculty of Medicine, Shiraz University of Medical Sciences, Fars, Iran

3 Department of Epidemiology, School of Medicine, Shiraz University of Medical Sciences, Fars, Iran


Introduction: Aflatoxins are secondary toxic metabolites produced by certain group of Aspergillus species in suitable conditions. These toxins are highly toxic, immunosuppressive, mutagenic, teratogenic and carcinogenic metabolites. The purpose of this study was to detection aflatoxin M 1 concentration in mother's milk from rural area of Khorrambid town of Fars Province.
Materials and Methods:
 In this study, 87 milk samples of mothers were collected by cluster sampling methods in the period between June and July 2011 and the amount of aflatoxin M 1 was measured by a competitive ELISA method.
Results: From 87 mother's milk, 24 (27.6%) samples were contaminated with aflatoxin M 1 with mean concentration of 0.56 ΁ 1.23 pg/ml (range 0.13-4.91 pg/ml).
Conclusion: The amount of aflatoxin M 1 in mothers' milk was lower than 50 ng/l (Europe Union and Iranian standard). Detection of Aflatoxin M 1 in mothers' milk is due to consuming contaminated food. This contamination not only threatens the health of the mothers but also has irreversible effects on the growth and health of their babies.


1. WHO, (World Health Organization). Global strategy for infant and young child feeding. Geneva, Switzerland: World Health Organization; 2003.  Back to cited text no. 1
2. Jensen AA, Slorach SA. Levels and trends of environmental chemicals in human milk. Boston: CRC Press; 1991.  Back to cited text no. 2
3. Jørgensen K, Rasmussen G, Thorup I. Ochratoxin A in Danish cereals 1986-1992 and daily intake by the Danish population. Food Addit Contam 1996;13:95-104.  Back to cited text no. 3
4. Speijers GJA, Van Egmond HP. Worldwide ochratoxin a levels in food and feeds. In: Creppy EE, Castegnaro M, Dirheimer G, editors. Human Ochratoxicosis and Its Pathologies. Vol. 231. Colloque INSERM/John Libbey Eurotext; 1993. p. 85-100.  Back to cited text no. 4
5. Zarba A, Wild CP, Hall AJ, Montesano R, Hudson GJ, Groopman JD. Aflatoxin M1 in human breast milk from The Gambia, west Africa, quantified by combined monoclonal antibody immunoaffinity chromatography and HPLC. Carcinogenesis 1992;13:891-4.  Back to cited text no. 5
6. el-Nezami HS, Nicoletti G, Neal GE, Donohue DC, Ahokas JT. Aflatoxin M1 in human breast milk samples from Victoria, Australia and Thailand. Food Chem Toxicol 1995;33:173-9.  Back to cited text no. 6
7. Galvano F, Pietri A, Bertuzzi T, Gagliardi L, Ciotti S, Luisi S, et al. Maternal dietary habits and mycotoxin occurrence in human mature milk. Mol Nutr Food Res 2008;52:496-501.  Back to cited text no. 7
8. Navas SA, Sabino M, Rodriguez-Amaya DB. Aflatoxin M(1) and ochratoxin A in a human milk bank in the city of São Paulo, Brazil. Food Addit Contam 2005;22:457-62.  Back to cited text no. 8
9. Abdulrazzaq YM, Osman N, Yousif ZM, Al-Falahi S. Aflatoxin M1 in breast-milk of UAE women. Ann Trop Paediatr 2003;23:173-9.  Back to cited text no. 9
10. IARC, (International Agency for Research on Cancer). Some naturally occurring substances: Food items and constituents, heterocyclic aromatic amines and mycotoxins. IARC Monograph on the Evaluation of Carcinogenic Risks to Humans. Vol. 56. Lyon, France: World Health Organization; 1993. p. 245-395.  Back to cited text no. 10
11. Dhanasekaran D, Shanmugapriya S, Thajuddin N. Aflatoxins and aflatoxicosis in human and animals In: Guevara-González RG, editor. Aflatoxins -Biochemistry and Molecular Biology Croatia: InTech. Croatia; 2011. p. 221-54.  Back to cited text no. 11
12. Wild CP, Pionneau FA, Montesano R, Mutiro CF, Chetsanga CJ. Aflatoxin detected in human breast milk by immunoassay. Int J Cancer 1987;40:328-33.  Back to cited text no. 12
13. Galvano F, Galofaro V, Galvano G. Occurrence and stability of aflatoxin M1 in milk and milk products: a worldwide review. J. Food Protect 1996;59:1079-90.  Back to cited text no. 13
14. Kuiper-Goodman T. Risk assessment of mycotoxins. In: S Natori, K Hashimoto, Y Ueno, editors. Mycotoxins and Phycotoxins. Amsterdam: Elsevier Science Publishers B. V.; 1989. p. 257-64.  Back to cited text no. 14
15. Sherif SO, Salama EE, Abdel-Wahhab MA. Mycotoxins and child health: The need for health risk assessment. Int J Hyg Environ Health 2009;212:347-68.  Back to cited text no. 15
16. Hendrickse RG, Maxwell SM. Aflatoxins and child health in the tropics. Toxin Reviews 1989;8:31-41. Available in: [Last accessed on Jun 24 2012].  Back to cited text no. 16
17. Wild CP, Rasheed FN, Jawla MF, Hall AJ, Jansen LA, Montesano R. In-utero exposure to aflatoxin in west Africa. Lancet 1991;337:1602.  Back to cited text no. 17
18. Gürbay A, Sabuncuoðlu SA, Girgin G, Sahin G, Yiðit S, Yurdakök M, et al. Exposure of newborns to aflatoxin M1 and B1 from mothers' breast milk in Ankara, Turkey. Food Chem Toxicol 2010;48:314-9.  Back to cited text no. 18
19. Mahdavi R, Nikniaz L, Arefhosseini SR, Vahed Jabbari M. Determination of aflatoxin M(1) in breast milk samples in Tabriz-Iran. Matern Child Health J 2010;14:141-5.  Back to cited text no. 19
20. Sadeghi N, Oveisi MR, Jannat B. Incidence of aflatoxin M1 in human breast milk in Tehran, Iran. Food control 2009;20:75-8. Available in: and ELSEVIER [Last accessed on Jun 24 2012].  Back to cited text no. 20
21. Polychronaki N, West RM, Turner PC, Amra H, Abdel-Wahhab M, Mykkänen H, et al. A longitudinal assessment of aflatoxin M1 excretion in breast milk of selected Egyptian mothers. Food Chem Toxicol 2007;45:1210-5.  Back to cited text no. 21
22. Polychronaki N, C Turner P, Mykkänen H, Gong Y, Amra H, Abdel-Wahhab M, et al. Determinants of aflatoxin M1 in breast milk in a selected group of Egyptian mothers. Food Addit Contam 2006;23:700-8.  Back to cited text no. 22
23. Lamplugh SM, Hendrickse RG, Apeagyei F, Mwanmut DD. Aflatoxins in breast milk, neonatal cord blood, and serum of pregnant women. Br Med J (Clin Res Ed) 1988;296:968.  Back to cited text no. 23
24. Heidarinia A. Detection of aflatoxin M1 in breast milk. PhD Thesis. Ahvaz, Iran Faculty of Medicine, Ahvaz University; 1995.  Back to cited text no. 24