Taurine improves the wound healing process in cutaneous leishmaniasis in mice model, based on stereological parameters

Authors

1 Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran

2 Department of Parasitology and Mycology, Shiraz University of Medical Sciences, Shiraz, Iran

3 Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

Abstract

Background: Cutaneous Leishmaniasis is a self-limiting disease caused by protozoan parasites of the genus Leishmania, which affects the skin with full-thickness wounds, which are prone to scar formation even after treatment. Taurine (Tu) is one of the most abundant amino acids that has antioxidant and anti-inflammatory effects, which play an important role in the process of wound healing. Herein, we have investigated the effects of Tu on cutaneous Leishmaniasis wounds and L. major promastigotes.
Materials and Methods: Eighteen mice were induced with Leishmaniasis wounds (with L. Major) on the base of their tails and divided into three groups, T1: Treated with Tu injection, T2: Treated with Tu gel, and C: No treatment. Treatments were carried out every 24 hours for 21 days. The volume densities of the collagen bundles and vessels, vessel's length density and diameter, and fibroblast populations were estimated by stereological methods. Flow cytometry was used in order to investigate the direct Tu effect on parasites. The Mann-Whitney U test was used and P ≤ 0.05 was considered to be statistically significant.
Results: The numerical density of the fibroblasts, volume density of the collagen bundles, and length densities of the vessels in groups T1 and T2 were significantly higher than in group C (P < 0.05). The fibroblast numerical density of group T1 was higher than that of group T2 (P = 0.02). Incidentally, Tu had no direct effect on L. major parasites according to the flow cytometry analysis.
Conclusion: Tu showed the ability to improve the wound healing process and tissue regeneration although it had no direct anti-leishmaniasis effect.

Keywords

1. Leishmaniasis: Worldwide epidemiological and drug access update. Abstract As part of a World Health Organization. Available from http://www.who.int/leishmaniasis/resources/Leishmaniasis_worldwide_epidemiological_and_drug_access_update.pdf. [Last accessed on March 2010].  Back to cited text no. 1
    
2. Bates PA. Transmission of Leishmania metacyclic promastigotes by phlebotomine sand flies. Iran J Parasitol 2007;37:1097-106.  Back to cited text no. 2
    
3. Pourmohammadi B, Motazedian M, Hatam G, Kalantari M, Habibi P, Sarkari B. Comparison of three methods for diagnosis of cutaneous leishmaniasis. Iran J Parasitol 2010;5:1-8.  Back to cited text no. 3
    
4. Farahmand M, Nahrevanian H, Shirazi HA, Naeimi S, Farzanehnejad Z. An overview of a diagnostic and epidemiologic reappraisal of cutaneous leishmaniasis in Iran. Braz J Infect Dis 2011;15:17-21.  Back to cited text no. 4
    
5. Nilforoushzadeh MA, Jaffary F, Ansari N, Moradi S, Siadat AH. The comparison between trichloroacetic acid 50% and co(2) laser in the treatment of cutaneous leishmaniasis scar. Indian J Dermatol 2011;56:171-3.  Back to cited text no. 5
[PUBMED]  Medknow Journal  
6. Akhavan AA, Yaghoobi-Ershadi MR, Hasibi F, Jafari R, Abdoli H, Arandian MH, et al. Emergence of cutaneous leishmaniasis due to Leishmania major in a new focus of southern Iran. J Arthrop Borne Dis 2007;1:1-8.  Back to cited text no. 6
    
7. Markle WH, Makhoul K. Cutaneous leishmaniasis: Recognition and treatment. Am Fam Physician 2004;69:1455-60.  Back to cited text no. 7
    
8. von Stebut E. Cutaneous leishmania infection: Progress in pathogenesis research and experimental therapy. Exp Dermatol 2007;16:340-6.  Back to cited text no. 8
    
9. Aguiar MG, Pereira AM, Fernandes AP, Ferreira LA. Reductions in skin and systemic parasite burdens as a combined effect of topical paromomycin and oral miltefosine treatment of mice experimentally infected with leishmania (leishmania) amazonensis. Antimicrob Agents Chemother 2010;54:4699-704.  Back to cited text no. 9
    
10. Goto H, Lindoso JA. Current diagnosis and treatment of cutaneous and mucocutaneous leishmaniasis. Expert Rev Anti Infect Ther 2010;8:419-33.  Back to cited text no. 10
    
11. Nilforoushzadeh MA, Esfahani MH, Fesharaki MA, Siadat AH, Ansari N, Baradaran EH. Treatment of atrophic cutaneous leishmaniasis scar using autologous fibroblasts and keratinocytes (a case report and literature review). J Res Med Sci 2010;15:125-6.  Back to cited text no. 11
[PUBMED]  Medknow Journal  
12. Marcinkiewicz J, Kontny E. Taurine and inflammatory diseases. Amino Acids 2012.  Back to cited text no. 12
    
13. Marcinkiewicz J, Wojas-Pelc A, Walczewska M, Lipko-Godlewska S, Jachowicz R, Maciejewska A, et al. Topical taurine bromamine, a new candidate in the treatment of moderate inflammatory acne vulgaris: A pilot study. Eur J Dermatol 2008;18:433-9.  Back to cited text no. 13
    
14. Deðim Z, Celebi N, Sayan H, Babül A, Erdoðan D, Take G. An investigation on skin wound healing in mice with a taurine-chitosan gel formulation. Amino Acids 2002;22:187-98.  Back to cited text no. 14
    
15. Tian X, Zhang Z, Wang S, Diao Y, Zhao Z, Lv D. Copper-taurine (CT): A potential organic compound to facilitate infected wound healing. Med Hypotheses 2009;73:1048-50.  Back to cited text no. 15
    
16. Ashkani-Esfahani S, Emami Y, Esmaeilzadeh E, Bagheri F, Namazi MR, Keshtkar M, et al. Silymarin enhanced fibroblast proliferation and tissue regeneration in full thickness skin wounds in rat models; a stereological study. J Saudi Soc Dermatol Dermatol Surg 2013;17:7-12.  Back to cited text no. 16
    
17. Ashkani-Esfahani S, Imanieh MH, Khoshneviszadeh M, Meshksar A, Noorafshan A, Geramizadeh B, et al. The healing effect of arnebia euchroma in second degree burn wounds in rat as an animal model. Iran Red Crescent Med J 2012;14:70-4.  Back to cited text no. 17
    
18. Sakthianandeswaren A, Elso CM, Simpson K, Curtis JM, Kumar B, Speed TP, et al. The wound repair response controls outcome to cutaneous leishmaniasis. Proc Natl Acad Sci U S A 2005;102:15551-6.  Back to cited text no. 18
    
19. Kocyigit A, Keles H, Selek S, Guzel S, Celik H, Erel O. Increased DNA damage and oxidative stress in patients with cutaneous leishmaniasis. Mutat Res 2005;585:71-8.  Back to cited text no. 19
    
20. Dinçer S, Babül A, Erdoðan D, Ozoðul C, Dinçer SL. Effect of taurine on wound healing. Amino Acids 1996;10:59-71.  Back to cited text no. 20
    
21. Gültekin SE, Sengüven B, Sofuoðlu A, Taner L, Koch M. Effect of the topical use of the antioxidant taurine on the two basement membrane proteins of regenerating oral gingival epithelium. J Periodontol 2012;83:127-34.  Back to cited text no. 21
    
22. Zakai HA, Zimmo SK. Effects of itraconazole and terbinafine on leishmaniasis major leisons in BALB/c mice. Ann Trop Med Parasitol 2000;94:787-91.  Back to cited text no. 22
    
23. Lai A Fat EJ, Vrede MA, Soetosenojo RM, Lai A Fat RF. Pentamidine, the drug of choice for the treatment of cutaneous leishmaniasis in Surinam. Int J Dermatol 2002;41:796-800.  Back to cited text no. 23
    
24. Soto J, Toledo J, Gutierrez P, Nicholls RS, Padilla J, Engel J, et al. Treatment of American cutaneous leishmaniasis with miltefosine, an oral agent. Clin Infect Dis 2001;33:E57-61.  Back to cited text no. 24
    
25. Aguiar MG, Pereira AM, Fernandes AP, Ferreira LA. Reductions in skin and systemic parasite burdens as a combined effect of topical paromomycin and oral miltefosine treatment of mice experimentally infected with leishmania (leishmania) amazonensis. Antimicrob Agents Chemother. 2010;54:4699-704.  Back to cited text no. 25