Advanced Biomedical Research

Degranulated mast cells and TNF-α in oral lichen planus and oral lichenoid reactions diseases

Authors

1 Department of Oral and Maxillofacial Medicine and Torabinejad Dental Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

2 Department of Oral Pathology, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran

3 Department of Oral and Maxillofacial Medicine, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: The objective of this study was to assess mast cells and TNF-α in oral lichen planus (OLP) and oral lichenoid reactions (OLR) patients as diagnostic marker to the differential diagnosis of OLP and OLR diseases.
Materials and Methods: In this cross-sectional study, samples were obtained from 30 OLP and 30 OLR patients, between June 2010 and March 2011 in Dental clinic of the University of Isfahan, Iran. Mast cells in the reticular layer of the lamina propria for samples were evaluated using toluidine blue method and immunohistochemical technique. The clinical relevant data taken into account were: demographical data, total number and degranulated mast cells, ratio of degranulated mast cells and TNF-α positive degranulated mast cells.
Results: In OLP and OLR, the total mast cells were 21.2 ± 7.9 and 20.3 ± 6.8, degranulated mast cells were 15.5 ± 6.9 and 19.2 ± 6.9, ratio of degranulated mast cells to total mast cells were 0.716 ± 0.067 and 0.946 ± 0.081, and TNF-α positive degranulated mast cells were 13.6 ± 6.3 and 17.1 ± 6.04, respectively. There was no significant difference for the total mast cells. But degranulated mast cells, ratio of degranulated mast cells and TNF-α positive degranulated mast cells in OLR were significantly higher than OLP patients.
Conclusions: Our results showed that the degranulated mast cells, ratio of degranulated mast cells and TNF-α in OLR was significantly more than OLP patients and these may be able to be used as diagnostic markers to the differential diagnosis of OLP and OLR.

Keywords

1. Greenberg M, Glick M, Ship J. Burket's Oral Medicine. 11 th ed. Hamilton, Canada: BC Decker Inc.;. 2008. p. 89-97.  Back to cited text no. 1
    
2. Sugermann PB, Savage NW, Seymour GJ, Walsh LJ. Is there a role for tumor necrosis factor-alpha (TNF-alpha) in oral lichen planus? J Oral Pathol Med 1996;25:219-24.  Back to cited text no. 2
    
3. Walsh LJ, Davis MF, Xu LJ, Savage NW. Relationship between mast cell degranulation and inflammation in the oral cavity. J Oral Pathol Med 1995;24:266-75.  Back to cited text no. 3
    
4. Walsh LJ, kaminer MS, Lazarvs GS, Lavker RM, Murphy GF. Role of laminin in Localization of human dermal mastcell. Lab Invest 1991;65:433-40.  Back to cited text no. 4
    
5. Walsh LJ, Lavker RM. Murphy GF. Determinants of immune cell trafficking in the skin. Lab Invest 1990;63:592-600.  Back to cited text no. 5
    
6. Kaminer MS, Lavker RM, Walsh LJ, Whitaker D, Zweiman B, Murphy GF. Extracellular Localization of human connective tissue mast cell granule contents. J Invest Dermatol 1991;96:857-63.  Back to cited text no. 6
    
7. Waldorf JA, Walsh LJ, Schechter NM, Murphy GF. Eearly cellular events during evolving cutaneons delayed hypersensitivity in humans. Am J Pathol 1997;138:477-86.  Back to cited text no. 7
    
8. Simark-Mattsson C, Bergenholtz G, Jontell M, Eklund C, Seymour GJ, Sugerman PB, et al. Distribution of interleukin-2,-4,-10, tumour necrosis factor-alpha and transforming growth factor-beta mRNAs in oral lichen planus. Arch Oral Biol 1999;44:499-507.  Back to cited text no. 8
    
9. Zhao ZZ, Savage NW, Sugerman PB, Walsh LJ. Mast cell/T cell interaction in Oral Lichen Planus. J Oral Pathol Med 2002;3:189-95.  Back to cited text no. 9
    
10. Zhao ZZ, Savage Nw, Walsh LJ. Association between mastcells and laminin in Oral Lichen Planus. J Oral Pathol Med 1998;27:163-7.  Back to cited text no. 10
    
11. Moughal NA, Adonogianaki E, Thornhill MH, Kinane DF. Endothelial cell leukocyte adhesion molecule-1 (ELAM-1) and intercellular adhesion molecule-1 (ICAM-1) expression in gingival tissue during health and experimentally-induced gingivitis. J Periodontal Res 1992;27:623-30.  Back to cited text no. 11
    
12. Walsh LJ, Savage NW, Ishii T, Seymour GI. ImmunoPathogenesis of Oral Lichen Planus. J Oral Pathol Med 1990;19:389-96.  Back to cited text no. 12
    
13. Nylander K, DaNielsen B, Fejerskov O, Dobell steen E. Expression of the endothelial Leukocyte adhesion molecule -1 (ELAM -1) on endothelial cells in experimental gingivitis in humans. J Periodontol 1993;64:355-7.  Back to cited text no. 13
    
14. Gemmell E, Walsh LJ, Savage NW, Seymour GJ. Adhesion molecule expression in chronic inflammatory periodontally disease tissue. J Periodontal Res 1993;29:46-53.  Back to cited text no. 14
    
15. Davis MF, Walsh LJ, Xu LJ, Savage NW. Adhesion molecule expression in dental Pulp. J Dent Res 1994;73:749.  Back to cited text no. 15
    
16. Davis MF, Walsh LJ, Savage NW. Adhesion molecule expression in periapical inflammation. J Dent Res 1993;72:667.  Back to cited text no. 16
    
17. Walsh LJ, Trinchieri G, Waldorf HA, Whitaker D, Murphy GF. Human dermal mast cells contain and release tumor necrosis factor á, which induces endothelial leukocyte adhesion molecule 1. Oral Dis 2007;88:420-4.  Back to cited text no. 17
    
18. Zhao ZZ, Sugerman PB, Zhao XJ, Walsh LJ, Savage NW. Mast cell degranulation and the role of T cell RANTES in oral lichen planus. Oral Dis 2001;7:246-51.  Back to cited text no. 18
    
19. Chen X, Ltu Z, Yue Q, Liu H, Wu Y, Li J. The expression of TNF á and ICAM-1 in lesions of lichen planus and its implication. J Huazhong Univ Sci Technol 2007;27:730-41.  Back to cited text no. 19
    
20. Zhang Y, Lin M, Zhang S, Wang Z, Jiang L, Shen J, et al. NF-kappaB-dependent cytokines in saliva and serum from patients with oral lichen planus: a study in an ethnic Chinese population. Cytokine 2008;41:144-9.  Back to cited text no. 20
    
21. Sklavounou A, Chrysomali E, Scorilas A, Karameris A. TNF-alpha expression and apoptosis-regulating proteins in oral lichen planus: a comparative immunohistochemical evaluation. J Oral Pathol Med 2000;29:370-5.  Back to cited text no. 21
    
22. Rhodus NL, Cheng B, Myers S, Bowles W, Ho V, Ondrey F. A comparison of the pro-inflammatory, NF-kappaB-dependent cytokines: TNF-alpha, IL-1-alpha, IL-6, and IL-8 in different oral fluids from oral lichen planus patients. Clin Immunol 2005;114:278-83.  Back to cited text no. 22
    
23. Rhodus NL, Cheng B, Ondrey F. Th1/Th2 cytokine ratio in tissue transudates from patients with oral lichen planus. Mediators Inflamm 2007;2007:19854.  Back to cited text no. 23
    
24. Patel N, Mohammadi A, Rhatigan R. A comparative analysis of mast cell quantification in five common dermatoses: lichen simplex chronicus, psoriasis, lichen planus, lupus, and insect bite/allergic contact dermatitis/nummular dermatitis. ISRN Dermatol 2012;2012:759630.  Back to cited text no. 24
    
25. Rad M, Hashemipoor MA, Mojtahedi A, Zarei MR, Chamani G, Kakoei S, et al. Correlation between clinical and histopathologic diagnoses of oral lichen planus based on modified WHO diagnostic criteria. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;107:796-800.  Back to cited text no. 25
    
26. Van der Meij EH, van der Waal I. Lack of clinicopathologic correlation in the diagnosis of oral lichen planus based on the presently available diagnostic criteria and suggestions for modifications. J Oral Pathol Med 2003;32:507-12.  Back to cited text no. 26
    
27. Ismail SB, Kumar SKS, Zain RB. Oral lichen planus and lichenoid reactions: etiopathogenesis, diagnosis, management and malignant transformation. J Oral Sci 2007;49:89-106.  Back to cited text no. 27
    
28. Abraham SN, St John AL. Mast cell-orchestrated immunity to pathogens. Nat Rev Immunol 2010;10:440-52.  Back to cited text no. 28
    
29. Galli SJ, Tsai M. Mast cells in allergy and infection:versatile effector and regulatory cells in innate and adaptive immunity. Eur J Immunol 2010;40:1843-51.  Back to cited text no. 29
    
30. Sharma R, Sircar K, Singh S, Rastogi V. Role of mast cells in pathogenesis of oral lichen planus. J Oral Maxillofac Pathol 2011;15:267-71.  Back to cited text no. 30
[PUBMED]  Medknow Journal  
31. Juneja M, Mahajan S, Rao NN, George T, Boaz K. Histochemical analysis of pathological alterations in oral lichen planus and oral lichenoid lesions. J Oral Sci 2006;48:185-93.  Back to cited text no. 31
    
32. Jahanshahi G, Aminzadeh A. A histochemical and immunohistochemical study of mast cells in differentiating oral lichen planus from oral lichenoid reactions. Quintessence Int 2010;41:221-7.  Back to cited text no. 32
    
Advanced Biomedical Research
Volume 2012, August
August 2012
Pages 1-5