Cholestasis progression effects on long-term memory in bile duct ligation rats


1 Department of Cognitive Neuroscience, Institute for Cognitive Science Studies, Tehran, Iran

2 Anesthesiology and Critical Care Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

3 Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

4 Department of Cognitive Neuroscience, Institute for Cognitive Science Studies; Department of Neuroscience, School of Advanced Medical Technologies and Department of Pharmacology, School of Medicine; Department of Addiction Studies, Iranian National Center for Addiction Studies, Tehran University of Medical Sciences, Tehran, Iran

5 Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Garmsar Branch, Garmsar, Iran


Background : There is evidence that cognitive functions are affected by some liver diseases such as cholestasis. Bile duct ligation induces cholestasis as a result of impaired liver function and cognition. This research investigates the effect of cholestasis progression on memory function in bile duct ligation rats.
Materials and Methods: Male Wistar rats were randomly divided into five groups, which include: control group for BDL-7, control group for BDL-21, sham group (underwent laparotomy without bile duct ligation), BDL-7 group (7 days after bile duct ligation), and BDL-21 group (21 days after bile duct ligation). Step-through passive avoidance test was employed to examine memory function. In all groups, short-term (7 days after foot shock) and long-term memories (21 days after foot shock) were assessed.
Results: Our results showed that liver function significantly decreased with cholestasis progression (P < 0.01). Also our findings indicated BDL-21 significantly impaired acquisition time (P < 0.05). Memory retrieval impaired 7 (P < 0.05) and 21 days (P < 0.001) after foot shock in BDL-7 and BDL-21 groups, respectively.
Conclusion: Based on these findings, liver function altered in cholestasis and memory (short-term and long-term memory) impaired with cholestasis progression in bile duct ligation rats. Further studies are needed to better insight the nature of progression of brain damage in cholestatic disease.


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