Effect of Echinophora platyloba , Stachys lavandulifolia, and Eucalyptus camaldulensis plants on Trichomonas vaginalis growth in vitro


1 Department of Parasitology and Mycology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

2 Department of Microbiology, Plant Research Center, Shahrekord, Iran

3 Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran


Background: Trichomonas vaginalis is a protozoan parasite which causes vaginitis in women worldwide. Metronidazole with vast side effects is drug of choice for this infection. In search for an alternative drug, in this study the effect of three plants on Trichomonas vaginalis has been investigated in vitro.
Materials and Methods: Alcoholic and watery extracts of Echinophora platylobaStachys lavandulifolia, and Eucalyptus camaldulensis were prepared. In TYIS culture medium containing alive Trichomonas vaginalis different concentrations of extracts of three plants were added. Following, 24, 48, and 72 h incubation the number of parasite in each test tube was counted.
Results: Eucalyptus camaldulensis showed a strong effect on Trichomonas vaginalis growth. However, no significant effect was observed with Echinophora platyloba or Stachys lavandulifolia extracts.
Conclusion: Eucalyptus camaldulensis can be considered as an alternative drug for treatment of infective vaginitis which is caused by bacteria, fungi and parasites.


1. Krieger JN. Consider diagnosis and treatment of trichomoniasis in men. Sex Transm Dis 2000;27:241-2.  Back to cited text no. 1
2. Petrin D, Delgaty K, Bhatt R, Garber G. Clinical and microbiological aspects of Trichomonas vaginalis. Clin Microbiol Rev 1998;11:300-17.  Back to cited text no. 2
3. King A, Nicol C. Venereal diseases. 7 & 8 Henrietta Street, London WC2E 8QE: Bailliére Tindall; 1975.  Back to cited text no. 3
4. Kuberski T. Trichomonas vaginalis associated with nongonococcal urethritis and prostatitis. Sex Transm Dis;1980;7:135-136.  Back to cited text no. 4
5. Cotch M, Pastorek JG, Nugent RP, Hillier SL, Gibbs RS, Martin DH, et al. Trichomonas vaginalis associated with low birth weight and preterm delivery. Sex Transm Dis 1997;24:353-60.  Back to cited text no. 5
6. Sorvillo F, Kovacs A, Kerndt P, Stek A, Muderspach L, Sanchez-Keeland L. Risk factors for trichomoniasis among women with human immunodeficiency virus (HIV) infection at a public clinic in Los Angeles County, California: Implications for HIV prevention. Am J Trop Med Hyg 1998;58:495-500.  Back to cited text no. 6
7. Durel P, Roiron V, Siboulet A, Borel LJ. Systemic treatment of trichomoniasis with a derivative of nitro-imidazole, 8823 RP. Br J Vener Dis 1960;36:21-6.  Back to cited text no. 7
8. Meingassner JG, Thurner J. Strain of Trichomonas vaginalis resistant to metronidazole and other 5-nitroimidazoles. Antimicrob Agents Chemother 1979;15:254-7.  Back to cited text no. 8
9. Entezari M, Hashemi M, Ashki M, Ebrahimian S, Bayat M, Azizi Saraji A. Studying the effect echinophora platyloba extract on bactira (staphilococus aureus and pseudomonas aeroginosa) and Fungi (Candidia albicans, Aspergilus flavus and Aspergilus niger) in vitro. World J Med Sci 2009;4:89-92.  Back to cited text no. 9
10. Rabbani M, Sajjadi S, Zarei H. Anxiolytic effects of Stachys lavandulifolia on the elevated plus-maze model of anxiety in mice. J Ethnopharmacol 2003;89:271-6.  Back to cited text no. 10
11. Falahati M, Tabrizib NO, Jahaniani F. Anti dermatophyte activities of Eucalyptus camaldulensis in comparison with Griseofulvin. Iranian Journal of Pharmacology & Therapeutics (IJPT) 2005;80-3.  Back to cited text no. 11
12. Adeniyi CB, Lawal TO, Mahady GB. In vitro susceptibility of Helicobacter pylori to extracts of Eucalyptus camaldulensis and Eucalyptus torelliana. Pharm Biol 2009;47:99-102.  Back to cited text no. 12
13. Javad SG, Atefeh A. Antimicrobial and antifungal properties of the essential oil and methanol extracts of Eucalyptus largiflorens and Eucalyptus intertexta. Pharmacogn Mag 2010;6:172-5.  Back to cited text no. 13
14. Mahdi N, Gany Z, Sharief M. Alternative drugs against Trichomonas vaginalis. East Mediterr Health J 2006;12:679-84.  Back to cited text no. 14
15. Nathan H, Abel S. Evaluation of in vitro activity of essential oils against trypanosoma brucei brucei and Trypanosoma evansi. J Parasitol Res 2010.  Back to cited text no. 15
16. Ramezani H, Singh H, Batish D, Kohli R. Antifungal activity of the volatile oil of Eucalyptus citriodora. Fitoterapia 2002;73:261-2.  Back to cited text no. 16
17. Sartorelli P, Marquioreto AD, Amaral Baroli A, Lima ME, Moreno PR. Chemical composition and antimicrobial activity of the essential oils from two species of Eucalyptus. Phytother Res 2007;21:231-3.  Back to cited text no. 17
18. Ghasemi Pirbalouti A, Malekpoor F, Enteshari S, Yousefi M, Momtaz H, Hamedi B. Antibacterial activity of some folklore medicinal plants used by Bakhtiari tribal in Southwest Iran. International Journal of Biology 2010; 55-64.  Back to cited text no. 18
19. Saeedi M, Morteza-Semnani K, Mahdavi M, Rahimi F. Antimicrobial studies on extracts of four species of Stachys. Indian J Pharm Sci 2008;70:403-6.  Back to cited text no. 19
[PUBMED]  Medknow Journal  
20. Avijgan M, Saadat M, Nilforoshzadeh M, Hafizi M. Antifungal effect of echinophora platyloba’s extract against candida albicans. Iranian J Pharmacol Res 2006;4:285-9.  Back to cited text no. 20