Advanced Biomedical Research
Fabrication and characterization of biodegradable polymeric films as a corneal stroma substitute
Authors
- Sahar Salehi 1
- Mohammadhossein Fathi 1
- Shaghayegh Haghjooy Javanmard 2
- Farnaz Barneh 2
- Mona Moshayedi 2
1 Department of Materials Engineering, Biomaterials Research Group, Isfahan University of Technology, Isfahan, Iran
2 Department of Physiology, Applied, Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Background: Biodegradable elastomeric materials such as poly glycerol sebacate (PGS) have gained much current attention in the field of soft tissue engineering. The present study reports the synthesis of PGS with molar ratios of 1:1, 2:3, and 3:2 of glycerol and sebacic acid via polycondensation reaction and tests the effect of PGS on human corneal epithelial (HCE) cells viability in vitro.
Materials and Methods: PGS films were prepared by the casting method. We tried to fabricate PGS with different compositions and various properties as being a viable alternative to the corneal stroma in cornea tissue engineering. The chemical properties of the prepared polymer were investigated by means of attenuated total reflectance - Fourier transform infrared spectroscopy (ATR-FTIR) analysis and the in vitro cytotoxicity was investigated by the Alamarblue method.
Results: The functional groups observed in the PGS FTIR spectrums of PGS with various molar ratios were the same. However, the main difference was the time of completing the cross-linking reaction. The PGS prepared by 2:3 ratio as a molar ratio had the fastest and the 3:2 ratio had the lowest cross-linking rate because of the higher amount of sebacic acid. Results of the Alamarblue cytotoxicity test assay showed no deleterious effect on HCE cell viability and proliferation.
Conclusions: PGS is a potentially good candidate material for corneal tissue engineering because of its lack of in vitro HCE cell toxicity.
Keywords
| 1. |
Whitcher JP, Scrinivasan M, Upadhyay MP. Prevention of corneal ulceration in the developing world. Int Ophthalmol Clin 2002;42:71-7.  |
| 2. |
Atala A. Tissue engineering of artificial organs. J Endourol 2000;14:49-57. |
| 3. |
Martina M, Hutmacher DW. Biodegradable polymers applied in tissue engineering research: A review. Polym Int 2007;56:145-57. |
| 4. |
Wang Y, Ameer GA, Sheppard BJ, Langer R. A tough biodegradable elastomer. Nat Biotechnol 2002;20:602-6. |
| 5. |
Sundback CA, Shyu JY, Wang Y, Faquin WC, Langer RS, Vacanti JP, et al. Biocompatibility analysis of poly (glycerol sebacate) as a nerve guide material. Biomaterials 2005;26:5454-64. |
| 6. |
Liu Q, Tian M, Ding T, Shi R, Zhang L. Preparation and characterization of a biodegradable polyester elastomer with thermal processing abilities. J Appl Polym Sci 2005;98:2033-41. |
| 7. |
Jaffar IH, Ammer MM, Jedlicka SS, Pearson RA, Coulter JP. Spectroscopic evaluation, thermal, and thermomechanical characterization of poly (glycerol-sebacate) with variations in curing temperatures and durations. J Mater Sci 2010;45:2525-9. |
| 8. |
Al-Nasiry S, Geusens N, Hanssens M, Luyten C, Pijnenborg R. The use of Alamar Blue assay for quantitative analysis of viability, migration and invasion of choriocarcinoma cells. Hum Reprod 2007;22:1304-9. |
| 9. |
Ravichandran R, Venugopal JR, Sundarrajan S, Mukherjee S, Sridhar R, Ramakrishna S. Expression of cardiac proteins in neonatal cardiomyocytes on PGS/fibrinogen core/shell substrate for Cardiac tissue engineering. Int J Cardiol 2012 [Epub ahead of print]. |
| 10. |
Chen QZ, Ishii H, Thouas GA, Lyon AR, Wright JS, Blaker JJ, et al. An elastomeric patch derived from poly (glycerol sebacate) for delivery of embryonic stem cells to the heart. Biomaterials 2010;31:3885-93. |
| 11. |
Chen QZ, Bismarck A, Hansen U, Junaid S, Tran MQ, Harding SE, et al. Characterisation of a soft elastomer poly (glycerol sebacate) designed to match the mechanical properties of myocardial tissue. Biomaterials 2008;29:47-57. |
| 12. |
Redenti S, Neeley WL, Rompani S, Saigal S, Yang J, Klassen H, et al. Engineering retinal progenitor cell and scrollable poly (glycerol-sebacate) composites for expansion and subretinal transplantation. Biomaterials 2009;30:3405-14. |
| 13. |
Sun ZJ, Chen C, Sun MZ, Ai CH, Lu XL, Zheng YF, et al. The application of poly (glycerol-sebacate) as biodegradable drug carrier. Biomaterials 2009;30:5209-14.
|
)