Antihyperglycemic and antihyperlipidemic effects of hydroalcoholic extract of Securigera securidaca seeds in streptozotocin-induced diabetic rats

Rajaei, Ziba; Hadjzadeh, Mousa-Al-Reza; Moradi, Reyhaneh; Ghorbani, Ahmad; Saghebi, Ahmad

Antihyperglycemic and antihyperlipidemic effects of hydroalcoholic extract of Securigera securidaca seeds in streptozotocin-induced diabetic rats

Authors

¹ Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

² Neurocognitive Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

³ Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

⁴ Department of Iranian Traditional Medicine, School of Traditional Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

Background: Hyperlipidemia is an associated complication of diabetes mellitus. Lowering of serum lipid levels seems to be associated with a decrease in the risk of vascular disease and related complications. The purpose of the current study was to evaluate the antihyperglycemic and antihyperlipidemic effects of the hydroalcoholic extract of Securigera securidaca seeds in streptozotocin-induced diabetic rats.
Materials and Methods: Female Wistar rats were randomly divided into four groups as follows: Control, diabetic, and diabetic rats treated with the Securigera extract at doses of 100 and 200 mg/kg. The animals were rendered diabetic by a single intraperitoneal injection of 55 mg/kg streptozotocin. Diabetic rats received the Securigera extract daily in drinking water from the day on which diabetes was confirmed for 4 weeks. The levels of serum glucose and lipids were spectrophotometrically measured in all groups at weeks 0 (before diabetes induction), 2, and 4.
Results: The results showed that there was a significant increase in serum glucose, triglycerides, total cholesterol, and low density lipoprotein (LDL)-cholesterol in streptozotocin-induced diabetic rats, accompanied by a decrease in high density lipoprotein (HDL)-cholesterol. Treatment of diabetic rats with S. securidaca seed extract at a dose of 200 mg/kg over a 4-week period significantly reduced the levels of serum glucose, total cholesterol, and LDL-cholesterol and increased the level of HDL-cholesterol, compared to diabetic untreated rats.
Conclusions: Securigera extract at a dose of 200 mg/kg exhibited hypoglycemic and hypolipidemic activities in streptozotocin-diabetic rats during the 4-week treatment period. This provides a valid scientific basis for using it in the treatment of diabetes in Iranian folk medicine.

Keywords

References

1.	Kim SH, Hyun SH, Choung SY. Anti-diabetic effect of cinnamon extract on blood glucose in db/db mice. J Ethnopharmacol 2006;104:119-23.
2.	American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care 2013;36:S67-74.
3.	Betteridge J. Lipid disorders in diabetes mellitus. In: Pickup JC, Williams G, editors. Text Book of Diabetes. London: Blackwell Science Publishers; 1997.
4.	Gupta RK, Kesari AN, Murthy PS, Chandra R, Tandon V, Watal G. Hypoglycemic and antidiabetic effect of ethanolic extract of leaves of Annona squamosa L. in experimental animals. J Ethnopharmacol 2005;99:75-81.
5.	Rajaei Z, Hadjzadeh MA, Nemati H, Hosseini M, Ahmadi M, Shafiee S. Antihyperglycemic and antioxidant activity of crocin in streptozotocin-induced diabetic rats. J Med Food 2013;16:206-10.
6.	Akhlaghi F, Rajaei Z, Hadjzadeh MA, Iranshahi M, Alizadeh M. Antihyperglycemic effect of Asafoetida (Ferula asafoetida oleo-gum-resin) in streptozotocin-induced diabetic rats. World Appl Sci J 2012;17:157-62.
7.	Zatula VV, Chernobrovaya NV, Kolesnikov DG. The structure of securigenin and its bioside securidaside. Chem Nat Compd 1966;2:438-9.
8.	Hosseinzadeh H, Ramezani M, Danaei AR. Antihyperglycaemic effect and acute toxicity of Securigera securidaca L. seed extracts in mice. Phytothe Res 2002;16:745-7.
9.	Amini G, editor. Iranian traditional medicinal plants (in Persian). Tehran: Medicinal Plants Research Institute; 1991.
10.	Al-Hachim GM, Maki B. Effect of Securigera Securidaca on electroshock seizure threshold in mice. Psychol Rep 1969;24:551-3.
11.	Ali AA, Mohamed MH, Kamel MS, Fouad MA, Spring O. Studies on Securigera securidacea (L.) Deg. et Dörfl. (Fabaceae) seeds, an antidiabetic Egyptian folk medicine. Pharmazie 1998;53:710-5.
12.	Mard SA, Bahari Z, Eshaghi N, Farbood Y. Antiulcerogenic effect of Securigera securidaca L. seed extract on various experimental gastric ulcer models in rats. Pak J Biol Sci 2008;11:2619-23.
13.	Pouramir M, Shahaboddin ME, Moghadamnia AA, Parastouei K. To study the effects of Securigera securidaca (L.) seed against alloxan-induced hyperglycemia. J Med Plants Res 2011;5:3188-91.
14.	Garjani A, Fathiazad F, Zakheri A, Allaf Akbari N, Azarmie Y, Fakhrjoo A, et al. The effect of total extract of Securigera securidaca L. seeds on serum lipid profiles, antioxidant status, and vascular function in hypercholesterolemic rats. J Ethnopharmacol 2009;126:525-32.
15.	Friedewald WT, Levy RI, Fredrickson DS. In: Tietz, editor. Determination of LDL cholesterol. New York: Text Book of Clinical Biochemistry; 1972.
16.	Minaiyan M, Moattar F, Vali A. Effect of Securigera securidaca seeds on blood glucose level of normal and diabetic rats. Iran J Pharm Sci 2006;2:151-6.
17.	Shew WH, Jeng CY, Lee WJ, Lin SY, Pei D, Chen YT. Simvastatin treatment in postprandial hypertriglyceridemia in type 2 diabetes mellitus patients with combined hyperlipidemia. Metabolism 2001;50:355-9.
18.	Bagdade JD, Helve E, Taskinen MR. Effect of continuous insulin infusion therapy lipoprotein surface and core lipid composition in IDDM. Metabolism 1991;40:445-9.
19.	Pushparaj P, Tan CH, Tan BK. Effects of Averrhoabilimli leaf extract on blood glucose and lipids in streptozotocin diabetic rats. J Ethnopharmacol 2000;72:69-76.
20.	Al-Shamaony L, Al-Khazraji SM, Twaiji IA. Hypoglycemic effect of Artemisia herba alba. II. Effect of a valuable extract on some blood parameters in diabetic animals. J Ethnopharmacol 1994;43:167-71.
21.	Agardh CD, Bjorgell P, Nilson EP. The effect of tolbutamide on lipoproteins, and lipoproteinlipase and hormone sensitive lipase. Diabetes Res Clin Pract 1999;46:99-108.
22.	Bopanna KN, Kannan J, Sushma G, Balaraman R, Rathod SP. Antidiabetic and anti hyperlipaemic effects of neem seed kernel powder on alloxan diabetic rabbits. Indian J Pharmacol 1997;29:162-7.
23.	Temme EH, Van Hoydonck PG, Schouten EG, Kesteloot H. Effects of a plant sterol-enriched spread on serum lipids and lipoproteins in mildly hypercholesterolemic subjects. Acta Cardiol 2002;57:111-5.
24.	Brown GB, Xue-Qiao Z, Sacco DE, Alberts JJ. Lipid lowering and plaque regression. New insights into prevention of plaque disruption and clinical events in coronary disease. Circulation 1993;87:1781-91.
25.	Wilson PW. High density lipoprotein, low density lipoprotein and coronary heart disease. Am J Cardiol 1990;66:7A-10.
26.	Kritchevsky D. Fiber, lipids and atherosclerosis. Am J Clin Nutr 1978;31S: 65-74.
27.	Kelly JJ, Tsai AC. Effect of pectin, gum Arabic and agar on cholesterol absorption, synthesis and turnover in rats. J Nutr 1978;108:630-9.
28.	Kedar P, Chakrabarti CH. Effects of bittergourd (Momordica charantia) seed and glibenclamide in streptozotocin induced diabetes mellitus. Indian J Exp Biol 1982;20:232-5.
29.	Sharma SB, Nasir A, Prabhu KM, Murthy PS, Dev G. Hypoglycaemic and hypolipidemic effect of ethanolic extract of seeds of Eugenia jambolana in alloxan-induced diabetic rabbits. J Ethnopharmacol 2003;85:201-6.
30.	Slater HR, Packard CJ, Bicker S, Shephered J. Effects of cholestyramine on receptor mediated plasma clearance and tissue uptake of human low density lipoprotein in the rabbit. J Biol Chem 1980;255:10210-3.
31.	Vessal M, Hemmati M, Vasei M. Antidiabetic effects of quercetin in streptozotocin-induced diabetic rats. Comp Biochem Physiol C Toxicol Pharmacol 2003;135C: 357-64.
32.	Bhavna S, Chandrajeet B, Partha R. Hypoglycemic and hypolipidemic effects of flavonoid rich extract from Eugenia jambolana seeds on streptozotocin induced diabetic rats. Food Chem Toxicol 2008;46:2376-83.
33.	Francis G, Kerem Z, Makkar HP, Becker K. The biological action of saponins in animal systems: A review. Br J Nutr 2002;88:587-605.
34.	Ma HY, Zhao ZT, Wang LJ, Wang Y, Zhou QL, Wang BX. Comparative study on anti-hypercholesterolemia activity of diosgenin and total saponin of dioscorea panthacia. China J Chin Materia Med 2002;27:528-31.

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