The antioxidant effects of silver, gold, and zinc oxide nanoparticles on male mice in in vivo condition

Negahdary, Masoud; Chelongar, Reyhaneh; Zadeh, Shahrzad Kabiri; Ajdary, Marziyeh

The antioxidant effects of silver, gold, and zinc oxide nanoparticles on male mice in in vivo condition

Authors

¹ Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran

² Department of Biology, Payame Noor University, Iran

³ Young Researchers & Elite Club, Khorasgan Branch, Islamic Azad University, Isfahan, Iran

Abstract

Background: We studied the effects of different doses of gold nanoparticles (AuNPs), silver nanoparticles (AgNPs), and zinc oxide nanoparticles (ZnONPs) on oxidative stress markers including glutathione peroxidase (GPX) and catalase (CAT) on male mice.
Materials and Methods: Male albino mice of Wistar strain (N = 60), weighing 17-32 g, were used for this study. The mice were randomly assigned to three classes such that in each class, there were four groups of which one was control and the other three groups were fed with ZnONPs and AgNPs at 500, 250, and 125 ppm concentration and AuNPs at 100, 50, and 25 ppm concentration for 15 days. The heart blood was taken to measure GPX and CAT enzyme activities at the end of the treatment.
Results: In male mice treated with AgNPs, the GPX and CAT activities were significantly increased, while significant decreases were seen in the GPX and CAT activities in mice treated with ZnONPs (P < 0.05) and in mice treated with AuNPs (P < 0.05).
Conclusion: The results of this study showed that AuNPs and ZnONPs caused decreased antioxidant enzyme activities, while nanosilver had the reverse effect and increased the antioxidant enzyme activities and caused decreased stress oxidative.

Keywords

References

1.	Shah MA. Formation of zinc oxide nanoparticles by the reaction of zinc metal with methanol at very low temperature. Afr Phys Rev 2008;2:106-9.
2.	Ji JH, Bae GN, Yun SH, Jung JH, Noh HS, Kim SS. Evaluation of a silver nanoparticle generator using a small ceramic heater for inactivation of S. epidermidis bioaerosols. Aerosol Sci Technol 2007;41:786-93.
3.	Dechsakulthorn FB, Hayes A, Bakand S, Joeng L, Winder C. In vitro cytotoxicity assessment of selected nanoparticles using human skin fibroblasts. (Alternatives to Animal Testing and Experimentation 2006;14:397-400.
4.	Qun Li, Shui-Lin Chen, Wan-Chao Jiang . Durability of nano ZnO antibacterial cotton fabric to sweat. J Appl Polym Sci 2007;103:412-6.
5.	Saito M. Antibacterial, deodorizing, and UV absorbing materials obtained with zinc oxide (ZnO) coated fabrics. J Ind Text 1993;23:150-64.
6.	Service RF. American Chemical Society meeting. Nanomaterials show signs of toxicity. Science 2003;300:243.
7.	Patra HK, Banerjee S, Chaudhuri U, Lahiri P, Dasgupta AK. Cell selective response to gold nanoparticles. Nanomedicine 2007; 3:111-9.
8.	Balasubramanian SK, Jittiwat J, Manikandan J, Ong CN, Yu LE, Ong WY. Biodistribution of gold nanoparticles and gene expression changes in the liver and spleen after intravenous administration in rat. Biomaterials 2010;31:2034-42.
9.	Guan ZZ. An experimental study of blood biochemical diagnostic indices for chronic fluorosis. Zhongghua Yua Fang Yi Xue Za Zhi 1991;25:33-5.
10.	Eisler R. A review of silver hazards to plants and animals. Proceedings of the 4 ^th international conference transport, fate and effects of silver in the environment. USA: Maryland University; 1996. p. 143-4.
11.	Barathmanikanth S, Kalishwaralal K, Sriram M, Pandian SR, Youn HS, Eom S, et al. Anti-oxidant effect of gold nanoparticles restrains hyperglycemic conditions in diabetic mice. J Nanobiotechnology 2010;8:16.
12.	Giugliano D, Ceriello A, Paolisso G. Oxidative stress and diabetic vascular complications. Diabetes Care 1996;19:257-67.
13.	Feldman EL, Stevens MJ, Greene DA. Pathogenesis of diabetic neuropathy. Clin Neurosci 1997;4:365-70.
14.	Ruggiero D, Lecomte M, Michoud E, Lagarde M, Wiernsperger N. Involvement of cell-cell interactions in the pathogenesis of diabetic retinopathy. Diabetes Metab 1997;23:30-4.
15.	Baynes JW. Role of oxidative stress in development of complications in diabetes. Diabetes 1991;40:405-12.
16.	Rotruck JT, Pope Al, Ganther HE, Swanson AB. Selenium: Biochemical roles as a component of glutathione peroxidase. Science 1973;179:588-90.
17.	Machiedo GW, Powell RJ, Rush BF Jr, Swislocki NI, Dikdan G. The incidence of decreased red blood cell deformability in sepsis and the association with oxygen free radical damage and multiple system organ failure. Arch Surg 1989;124:1386-9.
18.	Connor EE, Mwamuka J, Gole A, Murphy CJ, Wyatt MD. Gold nanoparticles are taken up by human cells but do not cause acute cytotoxicity. Small 2005;1:325-7.
19.	Khan JA, Pillai B, Das TK, Singh Y, Maiti S. Molecular effects of uptake of gold nanoparticles in HeLa cells. Chembiochem 2007;8:1237-40.
20.	Lasagna-Reeves C, Gonzalez-Romero D, Barria MA, Olmedo I, Clos A, Sadagopa Ramanujam VM, et al. Bioaccumulation and toxicity of gold nanoparticles after repeated administration in mice. Biochem Biophys Res Commun 2010; 393:649-55.
21.	Zhang XD, Wu HY, Wu D, Wang YY, Chang JH, Zhai ZB, et al. Toxicologic effects of gold nanoparticles in vivo by different administration routes. Int J Nanomedicine 2010;5:771-81.
22.	Hansen TM, Phillip N. A multifunctional cog in the life and death machine. American Association for the Advancement of Science, 2003;193:31.
23.	Hussain SM, Hess KL, Gearhart JM, Geiss KT, Schlager JJ. In vitro toxicity of nanoparticles in BRL 3A rat liver cells. Toxicol In Vitro 2005;19:975-83.
24.	Hao L, Chen L. Oxidative stress responses in different organs of carp (Cyprinus carpio) with exposure to ZnO nanoparticles. Ecotoxicol Environ Saf 2012;80:103-10.
25.	Zhao X, Wang S, Wu Y, You H, Lv L. Acute ZnO nanoparticles exposure induces developmental toxicity, oxidative stress and DNA damage in embryo-larval zebrafish. Aquat Toxicol 2013;136:49-59.
26.	Tsoli M, Kuhn H, Brandau W, Esche H, Schmid G. Cellular uptake and toxicity of Au55 clusters. Small 2005;1:841-4.
27.	De Jong WH, Hagens WI, Krystek P, Burger MC, Sips AJ, Geertsma RE. Particle size-dependent organ distribution of gold nanoparticles after intravenous administration. Biomaterials 2008;29:1912-9.

Advanced Biomedical Research