Advanced Biomedical Research

Genetic characterization of Pseudomonas aeruginosa-resistant isolates at the university teaching hospital in Iran

Authors

1 Department of Microbiology, School of Medicine, Isfahan University of Medical sciences, Isfahan, Iran

2 Department of Microbiology, School of Medicine, Zahedan University of Medical Sciences, Zahedan; Department of Microbiology, School of Medicine, Isfahan University of Medical sciences, Isfahan, Iran

3 Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Background: Pseudomonas aeruginosa is an opportunistic pathogen that is commonly responsible for nosocomial infections. The aim of this study was to perform a genotyping analysis of the Pseudomonas aeruginosa-resistant isolates by the multilocus sequence typing (MLST) method at the university teaching hospital in Iran.
Materials and Methods: Antimicrobial susceptibility was analyzed for P. aeruginosa isolates. Ceftazidime-resistant (CAZ res ) isolates with a positive double-disc synergy test were screened for the presence of extended-spectrum β-lactamase-encoding genes. Phenotypic tests to detect the metallo-β-lactamase strains of P. aeruginosa were performed on imipenem-resistant (IMP res ) isolates. Selected strains were characterized by MLST.
Results: Of 35 P. aeruginosa isolates, 71%, 45% and 45% of isolates were CAZ res , IMP res and multidrug resistant (MDR), respectively. Fifty-seven percent of the isolates carried the blaOXAgroup-1 . All the five typed isolates were ST235. Isolates of ST235 that were MDR showed a unique resistance pattern.
Conclusion: This study shows a high rate of MDR P. aeruginosa isolates at the university teaching hospital in Iran. It seems MDR isolates of P. aeruginosa ST235 with unique resistance pattern disseminated in this hospital.

Keywords

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Advanced Biomedical Research
Volume 2015, july
July 2015
Pages 1-7