Evaluation of tenascin expression in ameloblastoma, odontogenic keratocyst, and dentigerous cyst by immunohistochemistry

Authors
Narges Ghazi 1
Nasrollah Saghravanian 1
Majid Mirhashemi 1
Abolfazl Abedini Ardakani 2
1 Department of Oral and Maxillofacial Pathology, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran
2 School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran
Abstract
Background: The aim of this study was to evaluate the expression pattern of tenascin in ameloblastoma, odontogenic keratocyst, and dentigerous cyst.
Materials and Methods: The expression of tenascin was evaluated in microscopic slides of 42 paraffin blocks including 12 ameloblastomas, 15 odontogenic keratocysts, and 15 dentigerous cysts by immunohistochemistry. The expression of tenascin was examined in stroma, epithelium-connective tissue interface, and epithelium of the lesions by two pathologists semiquantitatively.
Results: Stromal expression of tenascin was higher in ameloblastomas than other groups. All the paired groups showed significant differences except comparison of odontogenic keratocysts and dentigerous cysts. Epithelial-mesenchymal interface expression of tenascin was significantly higher in ameloblastomas and odontogenic keratocysts than dentigerous cysts. All the paired groups showed significant differences except comparison of odontogenic keratocysts and ameloblastomas. Expression of tenascin in epithelial cells of ameloblastomas was focal whereas in odontogenic keratocysts and dentigerous cysts negative immunoreactivity was reported.
Conclusions: Expression of tenascin in these lesions suggests that it could play a role in epithelial-mesenchymal interaction. Higher expression of tenascin in ameloblastoma, can explain immaturity of its stroma and aggressive nature of this lesion compared with other studied groups. Moreover, higher expression of tenascin in epithelial-mesenchymal interface of odontogenic keratocyst compared with dentigerous cyst reveals its more immature and aggressive nature and high rate of recurrence.

Keywords

1.
Pappalardo M, Tsao C-K, Tsang ML, Zheng J, Chang Y-M, Tsai C-Y. Long-term outcome of patients with or without osseointegrated implants after resection of mandibular ameloblastoma and reconstruction with vascularized bone graft: Functional assessment and quality of life. J Plast Reconstr Aesthet Surg 2018;71:1076-85.  Back to cited text no. 1
    
2.
AlKhudair B, AlKhatib A, AlAzzeh G, AlMomen A. Bilateral dentigerous cysts and ectopic teeth in the maxillary sinuses: A case report and literature review. Int J Surg Case Rep 2019;55:117-20.  Back to cited text no. 2
    
3.
Cawson RA, Odell EW. Cawson's Essentials of Oral Pathology and Oral Medicine E-Book. Elsevier Health Sciences. China; 2017.  Back to cited text no. 3
    
4.
Regezi J, Sciubba J, Jordan R. Oral Pathology: Clinical Pathologic Correlations. In: Dolan J, editor. Elsevier. China; 2016.  Back to cited text no. 4
    
5.
Bilodeau EA, Collins BM. Odontogenic cysts and neoplasms. Surg Pathol Clin 2017;10:177-222.  Back to cited text no. 5
    
6.
Jaeger F, de Noronha MS, Silva MLV, Amaral MBF, Grossmann SdMC, Horta MCR, et al. Prevalence profile of odontogenic cysts and tumors on Brazilian sample after the reclassification of odontogenic keratocyst. J Craniomaxillofac Surg 2017;45:267-70.  Back to cited text no. 6
    
7.
Deshmukh SB, Sonawane K. Odontogenic keratocysts to keratocystic odontogenic tumor. IJADS 2019;5:09-15.  Back to cited text no. 7
    
8.
Lam EW. Inflammatory conditions of the jaws. White and Pharoah's Oral Radiology: Principles and Interpretation. Elsevier. China; 2018. p. 363.  Back to cited text no. 8
    
9.
Neville BW, Damm DD, Allen CM, Chi AC. Oral and Maxillofacial Pathology. Elsevier Health Sciences; 2015.  Back to cited text no. 9
    
10.
Gnepp DR, Bishop JA. Gnepp's Diagnostic Surgical Pathology of the Head and Neck E-Book. Elsevier Health Sciences. Canada; 2020.  Back to cited text no. 10
    
11.
Nanci A. Ten Cate's Oral Histology-E-Book: Development, Structure, and Function. Elsevier Health Sciences. China; 2017.  Back to cited text no. 11
    
12.
Roll L, Faissner A. Tenascins in CNS lesions. InSeminars in cell & developmental biology 2019 May 1 (Vol. 89, pp. 118-124). Academic Press.  Back to cited text no. 12
    
13.
Okada T, Suzuki H. The role of tenascin-C in tissue injury and repair after stroke. Front Immunol 2021;11:607587.  Back to cited text no. 13
    
14.
Carnelio S, Vij H. Expression of tenascin and nucleolar organizer region in ameloblastoma and ameloblastic fibroma. J Oral Pathol Med 2010;39:223-9.  Back to cited text no. 14
    
15.
de Medeiros AMC, Nonaka CFW, Galvão HC, de Souza LB, de Almeida Freitas R. Expression of extracellular matrix proteins in ameloblastomas and adenomatoid odontogenic tumors. Eur Arch Otorhinolaryngol 2010;267:303-10.  Back to cited text no. 15
    
16.
de Oliveira MDC, De Miranda JL, De Amorim RFB, De Souza LB, de Almeida Freitas R. Tenascin and fibronectin expression in odontogenic cysts. J Oral Pathol Med 2004;33:354-9.  Back to cited text no. 16
    
17.
da Silva MJA, de Sousa SOM, Corrêa L, Carvalhosa AA, de Araâaujo VC. Immunohistochemical study of the orthokeratinized odontogenic cyst: A comparison with the odontogenic keratocyst. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002;94:732-7.  Back to cited text no. 17
    
18.
Brellier F, Chiquet-Ehrismann R. How do tenascins influence the birth and life of a malignant cell? J Cell Mol Med 2012;16:32-40.  Back to cited text no. 18
    
19.
Howeedy A, Virtanen I, Laitinen L, Gould N, Koukoulis G, Gould V. Differential distribution of tenascin in the normal, hyperplastic, and neoplastic breast. Lab Invest 1990;63:798-806.  Back to cited text no. 19
    
20.
Kawakatsu H, Shiurba R, Obara M, Hiraiwa H, Kusakabe M, Sakakura T. Human carcinoma cells synthesize and secrete tenascin in vitro. Jpn J Cancer Res 1992;83:1073-80.  Back to cited text no. 20
    
21.
Becker J, Reichart P, Schuppan D, Philipsen H. Ectomesenchyme of ameloblastic fibroma reveals a characteristic distribution of extracellular matrix proteins. J Oral Pathol Med 1992;21:156-9.  Back to cited text no. 21
Advanced Biomedical Research
Volume 2023, March
March 2023
Pages 1-6