Absence of association between −286C>A>T polymorphism in the CRP gene and metabolic syndrome in Iranian pediatric

Authors

1 Applied Physiology Research Center; Child Growth and Development Research Center, Research Institute for Primordial Prevention of Non-communicable Disease; Department of Genetics and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

2 Department of Genetics, Faculty of Basic Sciences; Research Institute of Biotechnology, Shahrekord University, Shahrekord, Iran

3 Department of Genetics, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran

4 Child Growth and Development Research Center, Research Institute for Primordial Prevention of Non-communicable Disease, Isfahan, Iran

Abstract

Background: As a common pathophysiological condition worldwide, metabolic syndrome (MetS) is a clustering of multiple risk factors implicating in the development of many chronic disorders. Of note, obesity-induced chronic, low-grade inflammation is a major cause of insulin resistance and MetS. In the present study, we evaluated the association of rs3091244 variant of the C-reactive protein(CRP) gene, a well-recognized systemic inflammatory marker, with MetS in Iranian children and adolescents.
Materials and Methods: Genotyping was performed by mismatched polymerase chain reaction-restriction fragment length polymorphism in 100 MetS and 100 normal individuals aged 9–19 years recruited in the central part of Iran in 2011. A t-test or one-way ANOVA with post-hoc multiple comparisons were used to analyze the differences between groups. Statistical significance was defined as P ≤ 0.05. Logistic regression used to evaluate the association between alleles of the CRP rs3091244 and increased MetS risk.
Results: There were no differences in the genotype frequencies or allele distribution for −286C>A>T CRP polymorphism between MetS and control groups. Logistic regression showed that only the T allele of the CRP rs3091244 and not any of the genotypes confers a borderline significant (P = 0.059) increased MetS risk compared to A allele with the odds ratio of 1.70 (0.98–2.96).
Conclusions: This study suggests that in Iranian children and adolescents, −286C>A>T CRP polymorphism is not associated with the increased risk for MetS.

Keywords

1.
Alberti KG, Zimmet P, Shaw J. Metabolic syndrome – A new world-wide definition. A consensus statement from the International Diabetes Federation. Diabet Med 2006;23:469-80.  Back to cited text no. 1
    
2.
Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults. Executive Summary of the Third Report of the National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III). JAMA 2001;285:2486-97.  Back to cited text no. 2
    
3.
Isomaa B, Almgren P, Tuomi T, Forsén B, Lahti K, Nissén M, et al. Cardiovascular morbidity and mortality associated with the metabolic syndrome. Diabetes Care 2001;24:683-9.  Back to cited text no. 3
    
4.
Aschner P. Metabolic syndrome as a risk factor for diabetes. Expert Rev Cardiovasc Ther 2010;8:407-12.  Back to cited text no. 4
    
5.
Razzouk L, Muntner P. Ethnic, gender, and age-related differences in patients with the metabolic syndrome. Curr Hypertens Rep 2009;11:127-32.  Back to cited text no. 5
    
6.
Cruz ML, Goran MI. The metabolic syndrome in children and adolescents. Curr Diab Rep 2004;4:53-62.  Back to cited text no. 6
    
7.
Weiss R, Dziura J, Burgert TS, Tamborlane WV, Taksali SE, Yeckel CW, et al. Obesity and the metabolic syndrome in children and adolescents. N Engl J Med 2004;350:2362-74.  Back to cited text no. 7
    
8.
Kelishadi R, Ardalan G, Gheiratmand R, Adeli K, Delavari A, Majdzadeh R, et al. Paediatric metabolic syndrome and associated anthropometric indices: The CASPIAN Study. Acta Paediatr 2006;95:1625-34.  Back to cited text no. 8
    
9.
Schwandt P, Kelishadi R, Haas GM. Ethnic disparities of the metabolic syndrome in population-based samples of german and Iranian adolescents. Metab Syndr Relat Disord 2010;8:189-92.  Back to cited text no. 9
    
10.
Schwandt P, Kelishadi R, Ribeiro RQ, Haas GM, Poursafa P. A three-country study on the components of the metabolic syndrome in youths: The BIG Study. Int J Pediatr Obes 2010;5:334-41.  Back to cited text no. 10
    
11.
Calabro P, Yeh ET. Intra-abdominal adiposity, inflammation, and cardiovascular risk: New insight into global cardiometabolic risk. Curr Hypertens Rep 2008;10:32-8.  Back to cited text no. 11
    
12.
de Luca C, Olefsky JM. Inflammation and insulin resistance. FEBS Lett 2008;582:97-105.  Back to cited text no. 12
    
13.
Ridker PM. Clinical application of C-reactive protein for cardiovascular disease detection and prevention. Circulation 2003;107:363-9.  Back to cited text no. 13
    
14.
Festa A, D'Agostino R Jr, Howard G, Mykkänen L, Tracy RP, Haffner SM. Chronic subclinical inflammation as part of the insulin resistance syndrome: The Insulin Resistance Atherosclerosis Study (IRAS). Circulation 2000;102:42-7.  Back to cited text no. 14
    
15.
Crawford DC, Yi Q, Smith JD, Shephard C, Wong M, Witrak L, et al. Allelic spectrum of the natural variation in CRP. Hum Genet 2006;119:496-504.  Back to cited text no. 15
    
16.
Hage FG, Szalai AJ. C-reactive protein gene polymorphisms, C-reactive protein blood levels, and cardiovascular disease risk. J Am Coll Cardiol 2007;50:1115-22.  Back to cited text no. 16
    
17.
Szalai AJ, Wu J, Lange EM, McCrory MA, Langefeld CD, Williams A, et al. Single-nucleotide polymorphisms in the C-reactive protein (CRP) gene promoter that affect transcription factor binding, alter transcriptional activity, and associate with differences in baseline serum CRP level. J Mol Med (Berl) 2005;83:440-7.  Back to cited text no. 17
    
18.
Hsu LA, Chang CJ, Wu S, Teng MS, Chou HH, Chang HH, et al. Association between functional variants of the ICAM1 and CRP genes and metabolic syndrome in Taiwanese subjects. Metabolism 2010;59:1710-6.  Back to cited text no. 18
    
19.
Komurcu-Bayrak E, Erginel-Unaltuna N, Onat A, Ozsait B, Eklund C, Hurme M, et al. Association of C-reactive protein (CRP) gene allelic variants with serum CRP levels and hypertension in Turkish adults. Atherosclerosis 2009;206:474-9.  Back to cited text no. 19
    
20.
1000 Genomes Project Consortium, Abecasis GR, Altshuler D, Auton A, Brooks LD, Durbin RM, et al. A map of human genome variation from population-scale sequencing. Nature 2010;467:1061-73.  Back to cited text no. 20