Study of oxidants and antioxidants in patients of acute myocardial infarction

Authors
Mahesh Basavaraj Madole 1
Narendra Prabhakar Bachewar 2
Chandrashekhar M Aiyar 3
1 Department of Biochemistry, GMERS Medical College, Valsad, Gujarat, India
2 Department of Pharmacology, Shri VNGMC, Yavatmal, Maharashtra, India
3 Department of Biochemistry, BJMC, Pune, Maharashtra, India
Abstract
Background: Oxygen free radicals have become attractive candidates to explain injuries in ischemic heart. An association between raised serum uric acid concentration and increased cardiovascular risk has been recognized, however its role in acute myocardial infarction (AMI) is still unclear. Recently, zinc is also trying to establish its role in tissue injury and oxidative stress.
Materials and Methods: This cross-sectional study was carried on 75 AMI patients. 5 ml of blood was drawn from each patient within 6 h of AMI, to estimate plasma malondialdehyde (MDA), serum zinc, whole blood superoxide desmutase, serum uric acid, and whole blood glutathione peroxidase (GPx). The same biochemicals were also determined in 50 age and gender matched controls for comparison.
Results: We found significantly increased level of plasma MDA (5.649 ± 0.1780 vs. 2.757 ± 0.1623), serum uric acid (4.533 ± 0.1526 vs. 3.200 ± 0.1616) and significantly decreased levels of serum zinc (104.5 ± 1.874 vs. 115.3 ± 3.077), whole blood GPx (4599 ± 101.1 vs. 5519 ± 81.63) and superoxide desmutase (166.8 ± 1.896 vs. 188.3 ± 4.120). All the parameters studied also showed similar significant changes in male and female cases separately.
Conclusion: Raised MDA and decreased zinc, glutathione peroxidase, and superoxide desmutase levels denote the increased oxidative stress. Even being a defense, uric acid is raised as it is abundantly present in our body. Thus, AMI exhibits oxidative stress dependent changes irrespective of gender.

Keywords

1.
Nikolic-Heitzler V, Rabuzin F, Tatzber F, Vrkic N, Bulj N, Borovic S, et al. Persistent oxidative stress after myocardial infarction treated by percutaneous coronary intervention. Tohoku J Exp Med 2006;210:247-55.  Back to cited text no. 1
    
2.
Halliwell B. Tell me about free radicals, doctor: A review. J R Soc Med 1989;82:747-52.  Back to cited text no. 2
    
3.
Patil N, Chavan V, Karnik ND. Antioxidant status in patients with acute myocardial infarction. Indian J Clin Biochem 2007;22:45-51.  Back to cited text no. 3
    
4.
Waring WS. Uric acid: An important antioxidant in acute ischaemic stroke. QJM 2002;95:691-3.  Back to cited text no. 4
    
5.
Powell SR. The antioxidant properties of zinc. J Nutr 2000;130 5S Suppl: 1447S-54S.  Back to cited text no. 5
    
6.
Draper HH, Hadley M. Malondialdehyde determination as an index of lipid peroxidation. Methods Enzymol 1990;186:421-31.  Back to cited text no. 6
[PUBMED]    
7.
Homster R, Zak B. Determination of zinc in plasma. Clin Chem 1985;31:1310-3.  Back to cited text no. 7
    
8.
Henry RJ. Clinical Chemistry: Principles and Techniques. 2nd ed. New York: Harper and Row; 1974.  Back to cited text no. 8
    
9.
Arthur JR, Boyne R. Superoxide dismutase and glutathione peroxidase activities in neutrophils from selenium deficient and copper deficient mice. Life Sci 1985:70;1569-75.  Back to cited text no. 9
    
10.
Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967;70:158-69.  Back to cited text no. 10
[PUBMED]    
11.
Dey Sarkar P, Ramprasad N, Dey Sarkar I, Shivaprakash TM. Study of oxidative stress and trace element levels in patients with alcoholic and non-alcoholic coronary artery disease. Indian J Physiol Pharmacol 2007;51:141-6.  Back to cited text no. 11
    
12.
Shinde S, Kumar P, Patil N. Decreased levels of erythrocyte glutathione in patients with myocardial infarction. Internet J Altern Med 2004;2:1-4.  Back to cited text no. 12
    
13.
Jain AP, Mohan A, Gupta OP, Jajoo UN, Kalantri SP, Srivastava LM. Role of oxygen free radicals in causing endothelial damage in acute myocardial infarction. J Assoc Physicians India 2000;48:478-80.  Back to cited text no. 13
    
14.
Kairamkonda SR, Suryakar AN, Katkam RV, Ankush RD. Oxidative stress in acute myocardial infarction. Solapur Med J 2005;2:42-46  Back to cited text no. 14
    
15.
Gerritsen WB, van Boven WJ, Boss DS, Haas FJ, van Dongen EP, Aarts LP. Malondialdehyde in plasma, a biomarker of global oxidative stress during mini-CABG compared to on- and off-pump CABG surgery: A pilot study. Interact Cardiovasc Thorac Surg 2006;5:27-31.  Back to cited text no. 15
    
16.
Raghuvanshi R, Kaul A, Bhakuni P, Mishra A, Misra MK. Xanthine oxidase as a marker of myocardial infarction. Indian J Clin Biochem 2007;22:90-2.  Back to cited text no. 16
    
17.
Baynes JW. Oxygen and life. In: Baynes JW, Dominiczak MH, editors. Medical Biochemistry. 2nd ed. Philadelphia: Elsevier Mosby; 2005. p. 497-506.  Back to cited text no. 17
    
18.
Gomez E, del Diego C, Orden I, Elósegui LM, Borque L, Escanero JF. Longitudinal study of serum copper and zinc levels and their distribution in blood proteins after acute myocardial infarction. J Trace Elem Med Biol 2000;14:65-70.  Back to cited text no. 18
    
19.
Altekin E, Coker C, Sisman AR, Onvural B, Kuralay F, Kirimli O. The relationship between trace elements and cardiac markers in acute coronary syndromes. J Trace Elem Med Biol 2005;18:235-42.  Back to cited text no. 19
    
20.
Katayama T, Honda Y, Yamasaki H, Kitamura S, Okano Y. Serum zinc concentration in acute myocardial infaction. Angiology 1992; 43:791-2.  Back to cited text no. 20
    
21.
Lekakis J, Kalofoutis A. Zinc concentrations in serum as related to myocardial infarction. Clin Chem 1980;26:1660-1.  Back to cited text no. 21
[PUBMED]    
22.
Roussel AM, Kerkeni A, Zouari N, Mahjoub S, Matheau JM, Anderson RA. Antioxidant effects of zinc supplementation in Tunisians with type 2 diabetes mellitus. J Am Coll Nutr 2003;22:316-21.  Back to cited text no. 22
    
23.
Bos MJ, Koudstaal PJ, Hofman A, Witteman JC, Breteler MM. Uric acid is a risk factor for myocardial infarction and stroke: The Rotterdam study. Stroke 2006;37:1503-7.  Back to cited text no. 23
    
24.
Krishnan E, Baker JF, Furst DE, Schumacher HR. Gout and the risk of acute myocardial infarction. Arthritis Rheum 2006;54:2688-96.  Back to cited text no. 24
    
25.
Hayden MR, Tyagi SC. Uric acid: A new look at an old risk marker for cardiovascular disease, metabolic syndrome, and type 2 diabetes mellitus: The urate redox shuttle. Nutr Metab (Lond) 2004;1:10.  Back to cited text no. 25
    
26.
Dubois-Randé JL, Artigou JY, Darmon JY, Habbal R, Manuel C, Tayarani I, et al. Oxidative stress in patients with unstable angina. Eur Heart J 1994;15:179-83.  Back to cited text no. 26
    
27.
Werns SW, Shea MJ, Driscoll EM, Cohen C, Abrams GD, Pitt B, et al. The independent effects of oxygen radical scavengers on canine infarct size. Reduction by superoxide dismutase but not catalase. Circ Res 1985;56:895-8.  Back to cited text no. 27
[PUBMED]    
28.
Motghare KS, Bhutey A, Murrhar BB, Gupta M, Meshram AW, Balsubramanium Y. Lipid peroxidation and glutathione peroxidase in ischemic heart disease. Indian J Clin Biochem 2001;16:213-5.  Back to cited text no. 28
    
29.
Blankenberg S, Rupprecht HJ, Bickel C, Torzewski M, Hafner G, Tiret L, et al. Glutathione peroxidase 1 activity and cardiovascular events in patients with coronary artery disease. N Engl J Med 2003;349:1605-13.  Back to cited text no. 29
    
30.
Muzáková V, Kandár R, Vojtísek P, Skalický J, Cervinková Z. Selective antioxidant enzymes during ischemia/reperfusion in myocardial infarction. Physiol Res 2000;49:315-22.  Back to cited text no. 30
    
31.
Schnabel R, Lackner KJ, Rupprecht HJ, Espinola-Klein C, Torzewski M, Lubos E, et al. Glutathione peroxidase-1 and homocysteine for cardiovascular risk prediction: Results from the AtheroGene study. J Am Coll Cardiol 2005;45:1631-7.  Back to cited text no. 31
    
32.
Gutteridge JM. Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem 1995;41 (12 Pt 2):1819-28.  Back to cited text no. 32
    
33.
Young IS, Woodside JV. Antioxidants in health and disease. J Clin Pathol 2001;54:176-86.  Back to cited text no. 33
    
34.
Lewis P, Stefanovic N, Pete J, Calkin AC, Giunti S, Thallas-Bonke V, et al. Lack of the antioxidant enzyme glutathione peroxidase-1 accelerates atherosclerosis in diabetic apolipoprotein E-deficient mice. Circulation 2007;115:2178-87.  Back to cited text no. 34
    
35.
Shenkin A, Baines M, Fell GS, Lyon TDG. Vitamins and trace element. In: Burtis CA, Ashwood ER, Bruns DE, editors. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 4th ed. New Delhi: Saunders; 2006. p. 1137-44.  Back to cited text no. 35
Advanced Biomedical Research
Volume 2015, october
October 2015
Pages 1-6