Antinociceptive effect of palm date spathe hydroalcoholic extract on acute and chronic pain in mice as compared with analgesic effect of morphine and diclofenac


1 Department of Anatomy, Faculty of Medical Sciences, Yazd Branch, Islamic Azad University, Yazd, Iran

2 Department of Physiology, Herbal Medicine Research Center/Neurobiomedical Research Center, Yazd, Iran

3 Department of Surgery, Arak University of Medical Sciences and Health Services, Arak, Iran

4 Department of Physiology, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran

5 Department of Psychiatry, Kerman University of Medical Sciences and Health Services, Kerman, Iran


Backgrounds: In Persian traditional medicine, palm date spathe (PDS) is introduced as an analgesic. Therefore, this study was designed to investigate the analgesic effect of hydroalcoholic extract of PDS on acute and chronic pain in mice in comparison with diclofenac and morphine.
Materials and Methods: In this study, which was conducted in summer 2014, 220 male mice (20–30 g) were randomly divided into two categories, each consists of 11 groups as follows: A normal control group, a solvent (Tween 80) control group, 3 morphine positive control groups (2, 4 and 8 mg/kg), 3 diclofenac positive control groups (10, 20 and 30 mg/kg), and 3 main experimental PDS groups (2, 20, and 200 mg/kg). Hot plate was applied on animals in one category and writing test on the other category to assess acute and chronic pain, respectively.
Results: In the writing test, the average writing time and number of animals receiving a maximum dosage of morphine, diclofenac, and PDS were significantly less than the control group. In the hot plate test, only groups receiving different doses of morphine at different time points and those received 30 mg/kg diclofenac at 15 min after the intervention showed significant difference with the control group.
Conclusion: 200 mg/kg extract of PDS, revealed a significant analgesic effect on chronic pain, but it did not show any analgesic effect on acute pain.


Longo D, Fauci A, Kasper D, Hauser S, Jameson J, Loscalzo J. Harrison's Principles of Internal Medicine. 18th ed. New York: McGraw Hill Professional; 2012.  Back to cited text no. 1
Katzung B, Master S, Trever A. Basic and Clinical Pharmacology. 11th ed. New York: McGraw Hill; 2009.  Back to cited text no. 2
Naidoo V, Swan GE. Diclofenac toxicity in Gyps vulture is associated with decreased uric acid excretion and not renal portal vasoconstriction. Comp Biochem Physiol 2009;149:269-74.  Back to cited text no. 3
Mirheydar H. Herbal information usage of plants in preventaion and treatment of diseases. Tehran: Tehran University Publication; 2003.  Back to cited text no. 4
Rezaeisadrabadi M, Dashti RM, Anvari M, Falah-Tafti H, Zanbagh S. The evaluation of the analgesic effect of hydroalcoholic extract of solanum melongena in syrian mice using tail flick test. J Shahid Sadoughi Univ Med Sci 2011;19:490-500.  Back to cited text no. 5
Zargari, A. Iranian medicinal plants. Tehran: Tehran University Publications; 1997.  Back to cited text no. 6
Mokhtari M, Sharifi E, Moghadamnia D. Effect of alcoholic date spath on changes in testis tissue and hormones, FSH, LH and testosterone in male rats. IJBMS 2006;9:265-71.  Back to cited text no. 7
Mikki MS, Al-Taisan SM, Abdul-Aziz A. Isolation of the chemical constituents of the spathe of date palm. Saudi Arabia: Al-Hassa Regional Agricultural Research Center; 1989. p. 244-98.  Back to cited text no. 8
Vyawahare N, Pujari R, Khsirsagar A, Ingawale D, Patil M, Kagathara V. Phoenix dactylifera: An update of its indegenous uses, phytochemistry and pharmacology. Internet J Pharmacol 2009;7:4. Available from: [Last cited on 2014 Sep 16].  Back to cited text no. 9
Jahani A. Dates a fruit of life. Tehran: Agricultural Sciences Press; 2002.  Back to cited text no. 10
Fylaktakidou KC, Hadjipavlou-Litina DJ, Litinas KE, Nicolaides DN. Natural and synthetic coumarin derivatives with anti-inflammatory/antioxidant activities. Curr Pharm Des 2004;10:3813-33.  Back to cited text no. 11
Schenone S, Bruno O, Ranise A, Bondavalli F, Filippelli W, Falcone G, et al. O-[2-hydroxy-3-(dialkylamino) propyl] ethers of (+)-1,7,7-trimethyl bicyclo[2.2.1]heptan-2-one oxime (camphor oxime) with analgesic and antiarrhythmic activities. Farmaco 2000;55:495-8.  Back to cited text no. 12
Xu H, Blair NT, Clapham DE. Camphor activates and strongly desensitizes the transient receptor potential vanilloid subtype 1 channel in a vanilloid-independent mechanism. J Neurosci 2005;25:8924-37.  Back to cited text no. 13
Dashti-r MH, Vahidi AR, Panjalizadeh ME. Effect of Phoenix dactylifera spathe hydroalcoholic extract on chronic pain in mice. J Med Plants 2012;11:136-44.  Back to cited text no. 14
Samsamshariat SH. Extraction, separation, identification and structure elucidation of various types of natural products. Iran: Mani Press; 1992. p. 14-6.  Back to cited text no. 15
Heilborn U, Rost BR, Arborelius L, Brodin E. Arthritis-induced increase in cholecystokinin release in the rat anterior cingulate cortex is reversed by diclofenac. Brain Res 2007;1136:51-8.  Back to cited text no. 16
Mesdaghinia A, Samiei F. Investigation of stress induced analgesia with or without cck receptor agonists and antagonists in mice suffering from neuropathic pain using hot plate test. J Shahid Sadoughi Univ Med Sci 2004;12:39-42.  Back to cited text no. 17
Habibi-Asl B, Soltany R. Effect of ephedrine on morphine antinociception and tolerance induced to morphine antinociception in mice. Pharm Sci 2005;2:11-6.  Back to cited text no. 18
Koyama T, Fukuda K. Nociceptin receptor antagonist JTC-801 inhibits nitrous oxide-induced analgesia in mice. J Anesth 2009;23:301-3.  Back to cited text no. 19
Witaicenis A, Seito LN, da Silveira Chagas A, de Almeida Junior LD, Luchini AC, Rodrigues-Orsi P, et al. Antioxidant and intestinal anti-inflammatory effects of plant-derived coumarin derivatives. Phytomedicine 2014;21:240-6.  Back to cited text no. 20
Oh YC, Jeong YH, Cho WK, Ha JH, Gu MJ, Ma JY. Anti-Inflammatory and Analgesic Effects of Pyeongwisan on LPS-Stimulated Murine Macrophages and Mouse Models of Acetic Acid-Induced Writhing Response and Xylene-Induced Ear Edema. Int J Mol Sci 2015;16:1232-51.  Back to cited text no. 21
Yu S, Li S, Henke A, Welzel G, Li S, Cheng B,et al. Development of novel sterol-based Liver X Receptor agonists as therapeutics for inflammatory diseases (HUM1P. 307). J Immunol 2015;194 (1 Supplement):52.32.  Back to cited text no. 22
Loizou S, Lekakis I, Chrousos GP, Moutsatsou P. β-Sitosterol exhibits anti-inflammatory activity in human aortic endothelial cells. Molecular Nutrition And Food Research 2015;54:551-8.  Back to cited text no. 23
Abdel-Moein NM, Abdel-Moniem EA, Mohamed DA, Hanfy EA. Evaluation of the anti-inflammatory and anti-arthritic effects of some plant extracts. grasas y aceites 2015;62:365-74.  Back to cited text no. 24
Ribas CM, Meotti FC, Nascimento FP, Jacques AV, Dafre AL, Rodrigues AL, et al. Antinociceptive effect of the Polygala sabulosa hydroalcoholic extract in mice: Evidence for the involvement of glutamatergic receptors and cytokine pathways. Basic Clin Pharmacol Toxicol 2008;103:43-7.  Back to cited text no. 25
Al-Taher AY. Anticonvulsant effects of 3, 4-dimethoxy toluene, the major constituent of Phoenix dactylifera L spathe in mice. Sci J King Faisal Univ (Basic Appl Sci) 2008;9:115-25.  Back to cited text no. 26
Pujari RR, Vyawahare NS, Kagathara VG. Evaluation of antioxidant and neuroprotective effect of date palm (Phoenix dactylifera L.) against bilateral common carotid artery occlusion in rats. Indian J Exp Biol 2011;49:627-33.  Back to cited text no. 27
Vyawahare NS, Pujari RR, Rajendran R, Khsirsagar AD, Ingawale DK, Patil MN. Neurobehavioral effects of Phoenix dactylifera in mice. J Young Pharm 2009;1:225-32.  Back to cited text no. 28
Mohamed DA, Al-Okbi SY.In vivo evaluation of antioxidant and anti-inflammatory activity of different extracts of date fruits in adjuvant arthritis. Pol J Food Nutr Sci 2004;13:397-402.  Back to cited text no. 29
Bezerra MM, Lima V, Girão VC, Teixeira RC, Graça JR. Antinociceptive activity of sildenafil and adrenergic agents in the writhing test in mice. Pharmacol Rep 2008;60:339-44.  Back to cited text no. 30