Evaluation of differences in HLA-DR4 gene and its subtypes prevalence among healthy people and RA patients in Isfahan province population

Authors
Mansour Salesi 1
Golshan Taghipour Boroujeni 2
Mansoor Salehi 3
Somaieh Farzamnia 2
1 Department of Rheumatology, Isfahan University of Medical Sciences, Isfahan, Iran
2 Department of Internal Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Department of Genetics, Medical school, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Background: There are a lot of evidences showing that genetic play an important role in RA disease. Inheritance of some subgroups of HLA-DR4 gene increases the propensity to RA disease. In this paper, the impact of HLA-DR4 gene and its subtypes or subgroups, be consistence on RA patient who lived in Isfahan province, has been evaluated.
Materials and Methods: In this survey, two groups of people (100 patients in case group and 100 normal persons in control group) have been selected. These two groups were similar in age and gender. Statistical population has been considered among people who visited Al Zahra rheumatology clinic. The participants were from Isfahan province and accepted to participate to the study voluntarily. The prevalence of HLA-DR4 and its 0401-0404 subtypes were evaluated between two groups; DNA was extracted from blood samples and studied using PCR SSCP method.
Results: It was found that 35% of RA patients had HLA-DR4 gene, of which 14 persons had 0401, 10 persons had 0404, and 11 persons had other subtypes, whereas 30 people in control group had HLA-DR4 gene, of which 10 people had 0401, 20 people had 0404, and nobody had other subtypes.
Conclusion: The observed differences between prevalence of HLA-DR4 gene between the case and control group were not statistically significant (P = 0.45; OR = 1.256; 95% CI = 0.69-2.27), but a relation was between HLA-DR4 0404 subtypes and RA (P = 0.02; OR = 0.44; 95% CI = 0.196-0.992).

Keywords

1.
Longo DL, Fauci AS, Kasper DL, Hauser SL, Jameson JL, Loscalzo J, et al. Harrison's Principles of Internal Medicine, Disorders of the Immune System and Connective Tissue. 18 th ed. New York: McGraw-Hill; 2012.  Back to cited text no. 1
    
2.
De Rosa MC, Giardina B, Bianchi C, Carelli Alinovi C, Pirolli D, Ferraccioli G, et al. Modeling the ternary complex TCR-Vβ/CollagenII(261-273)/HLA-DR4 associated with rheumatoid arthritis.PLoS One 2010;5: e11550.   Back to cited text no. 2
    
3.
Lü Z, Li J, Feng ZT, Ren J. Association of HLA-DR4, PAD4, and STAT4 expression in the peripheral blood with disease activity in patients with rheumatoid arthritis. Nan Fang Yi Ke Da Xue Xue Bao 2010;30:1349-53.  Back to cited text no. 3
    
4.
Alvarez I, Collado J, Daura X, Colomé N, Rodríguez-García M, Gallart T, et al. The rheumatoid arthritis-associated allele HLA-DR10 (DRB1*1001) shares part of its repertoire with HLA-DR1 (DRB1*0101) and HLA-DR4 (DRB*0401). Arthritis Rheum 2008;58:1630-9.  Back to cited text no. 4
    
5.
Lee HS, Korman BD, Le JM, Kastner DL, Remmers EF, Gregersen PK, et al. Genetic risk factors for rheumatoid arthritis differ in Caucasian and Korean populations. Arthritis Rheum 2009;60:364-71.  Back to cited text no. 5
    
6.
Atouf O, Benbouazza K, Brick C, Bzami F, Bennani N, Amine B, et al. HLA polymorphism and early rheumatoid arthritis in the Moroccan population. Joint Bone Spine 2008;75:554-8.   Back to cited text no. 6
    
7.
Kim HY, Kim TG, Park SH, Lee SH, Cho CS, Han H. Predominance of HLA-DR4*0405 in Korean patients with rheumatoid arthritis. Ann Rheum Dis1995;54:988-90.  Back to cited text no. 7
    
8.
Nakai Y, Wakisaka A, Aizawa M, Itakura K, Nakai H, Ohashi A.HLA and rheumatoid arthritis in the Japanese. Arthritis Rheum1981;24:722-5.  Back to cited text no. 8
    
9.
Kapitány A, Zilahi E, Szántó S, Szücs G, Szabó Z, Végvári A, et al. Association of rheumatoid arthritis with HLA-DR1 and HLA-DR4 in Hungary. Ann N Y Acad Sci 2005;1051:263-70.   Back to cited text no. 9
    
10.
Adib M, Vahdati A. Relative frequency of HLA-DR and HLA-DQ in patients with rheumatoid arthritis. Med J Tabriz Univ Med Sci1999;33:3-6.  Back to cited text no. 10
    
11.
Shekarabi M, Nikbin B, Davachi F, Khosravi F. Association of HLA in selected group of Iranian patients with rheumatoid arthritis. Razi J Med Sci1994;1:20-4.   Back to cited text no. 11
    
12.
Jamshidi AR, Tehrani Banihashemi SA, Salari AH, Taghipour R. HLA-DR4 subtype relationship with rheumatoid arthritis: A study on Iranian patients. Tehran Univ Med J 2009;66:804-8.  Back to cited text no. 12
    
13.
Hameed K, Bowman S, Kondeatis E, Vaughan R, Gibson T. The association of HLA-DRB genes and the shared epitope with rheumatoid arthritis in Pakistan. Br J Rheumatol 1997;36:1184-8.  Back to cited text no. 13
    
14.
Tan PL, Farmiloe S, Roberts M, Geursen A, Skinner MA. HLA-DR4 subtypes in New Zealand Polynesians. Predominance of Dw13 in the healthy population and association of Dw15 with rheumatoid arthritis. Arthritis Rheum1993;36:15-9.   Back to cited text no. 14
    
15.
Thomson W, Harrison B, Ollier B, Wiles N, Payton T, Barrett J, et al. Quantifying the exact role of HLA-DRB1 alleles in susceptibility to inflammatory polyarthritis: Results from a large, population-based study. Arthritis Rheum1999;42:757-62.  Back to cited text no. 15
Advanced Biomedical Research
Volume 2016, January
January 2016
Pages 1-5