Phylogenetic analysis of otospiralin protein

Authors

1 Department of Molecular Geneti cs, Nati onal Insti tute of Geneti c Engineering and Biotechnology, Tehran, Iran

2 Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Fibrocyte-specific protein, otospiralin, is a small protein, widely expressed in the central nervous system as neuronal cell bodies and glia. The increased expression of otospiralin in reactive astrocytes implicates its role in signaling pathways and reparative mechanisms subsequent to injury. Indeed, otospiralin is considered to be essential for the survival of fibrocytes of the mesenchymal nonsensory regions of the cochlea. It seems that other functions of this protein are not yet completely understood.
Materials and Methods: Amino acid sequences of otospiralin from 12 vertebrates were derived from National Center for Biotechnology Information database. Phylogenetic analysis and phylogeny estimation were performed using MEGA 5.0.5 program, and neighbor-joining tree was constructed by this software.




Results: In this computational study, the phylogenetic tree of otospiralin has been investigated. Therefore, dendrograms of otospiralin were depicted. Alignment performed in MUSCLE method by UPGMB algorithm. Also, entropy plot determined for a better illustration of amino acid variations in this protein.
Conclusion: In the present study, we used otospiralin sequence of 12 different species and by constructing phylogenetic tree, we suggested out group for some related species.

Keywords

1.
Reilkoff RA, Bucala R, Herzog EL. Fibrocytes: Emerging effector cells in chronic inflammation. Nat Rev Immunol 2011;11:427-35.  Back to cited text no. 1
    
2.
Fan X, Liang HP. Circulating fibrocytes: A potent cell population in antigen-presenting and wound healing. Chin J Traumatol 2010;13:111-6.  Back to cited text no. 2
    
3.
Kao HK, Chen B, Murphy GF, Li Q, Orgill DP, Guo L. Peripheral blood fibrocytes: Enhancement of wound healing by cell proliferation, re-epithelialization, contraction, and angiogenesis. Ann Surg 2011;254:1066-74.  Back to cited text no. 3
    
4.
Bucala R. Fibrocytes: New Insights Into Tissue Repair and Systemic Fribrosis. Hardcover – January 30, 2007 by Richard Bucala.  Back to cited text no. 4
    
5.
Decourt B, Hillman D, Bouleau Y, Dulon D, Hafidi A. Is otospiralin inner ear specific? Evidence for its expression in mouse brain. Int J Dev Neurosci 2009;27:87-96.  Back to cited text no. 5
    
6.
Delprat B, Ruel J, Guitton MJ, Hamard G, Lenoir M, Pujol R, et al. Deafness and cochlear fibrocyte alterations in mice deficient for the inner ear protein otospiralin. Mol Cell Biol 2005;25:847-53.  Back to cited text no. 6
    
7.
Cohen-Salmon M, Frenz D, Liu W, Verpy E, Voegeling S, Petit C. Fdp, a new fibrocyte-derived protein related to MIA/CD-RAP, has an in vitro effect on the early differentiation of the inner ear mesenchyme. J Biol Chem 2000;275:40036-41.  Back to cited text no. 7
    
8.
Fransen E, Verstreken M, Verhagen WI, Wuyts FL, Huygen PL, D'Haese P, et al. High prevalence of symptoms of menière's disease in three families with a mutation in the COCH gene. Hum Mol Genet 1999;8:1425-9.  Back to cited text no. 8
    
9.
Rendtorff ND, Frödin M, Attié-Bitach T, Vekemans M, Tommerup N. Identification and characterization of an inner ear-expressed human melanoma inhibitory activity (MIA)-like gene (MIAL) with a frequent polymorphism that abolishes translation. Genomics 2001;71:40-52.  Back to cited text no. 9
    
10.
Delprat B, Boulanger A, Wang J, Beaudoin V, Guitton MJ, Ventéo S, et al. Downregulation of otospiralin, a novel inner ear protein, causes hair cell degeneration and deafness. J Neurosci 2002;22:1718-25.  Back to cited text no. 10
    
11.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731-9.  Back to cited text no. 11
    
12.
Edgar RC. MUSCLE: A multiple sequence alignment method with reduced time and space complexity. BMC Bioinformatics 2004;5:113.  Back to cited text no. 12