Stability of Vitamin D3 in fortified yoghurt and yoghurt drink (Doogh)

Authors
Tina Jafari 1
Gholamreza Askari 1
Maryam Mirlohi 2
Shaghayegh Haghjooy Javanmard 3
Elham Faghihimani 4
Aziz A Fallah 5
1 Department of Community Nutrition, Food Security Research Center, School of Nutrition and Food Science, Isfahan, Iran
2 Department of Food Science and Technology, Food Security Research Center, School of Nutrition and Food Science, Isfahan, Iran
3 Department of Physiology, Applied Physiology Research Center, Isfahan, Iran
4 Isfahan Endocrine and Metabolism Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
5 Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
Abstract
Background: Vitamin D deficiency and insufficiency are recognized as a worldwide problem with serious consequences. Fortification of foods with Vitamin D is a certain approach to improve serum Vitamin D status if the stability of vitamin in the foodstuffs was controlled. The purpose of this study was to examine the stability of Vitamin D3added to low-fat yogurt and yogurt drink “Doogh” during the products shelf-life.
Materials and Methods: Two kinds of Vitamin D3, water- and oil-dispersible forms, suitable for food fortification, were compared to find out whether they show different stability in the products. The products were packed in opaque or translucent containers. The content of Vitamin D3was determined by high performance liquid chromatography method.




Results: Vitamin D was not affected by the heat treatment (pasteurization) and other processes (homogenization and fermentation). Both water- and oil-dispersible forms were stable during the shelf-life of yogurt samples packed in opaque containers. The Vitamin D3content of yogurt fortified with water-dispersible form and packed in translucent containers was not stable during the shelf-life and significantly reduced after 1, 2, and 3 weeks of storage compared to the day 0. The Vitamin D3content of samples fortified with the oil-dispersible form packed in the same container was only stable after 1-week and significantly reduced after 2 and 3 weeks of storage. The Vitamin D3content of Doogh packed in the opaque containers remained stable during the shelf-life while it was not stable in the samples packed in translucent containers.
Conclusion: The results suggested that both forms of Vitamin D are suitable for fortification, and opaque container is a better choice for packaging of the product.

Keywords

1.
Kamen DL, Tangpricha V. Vitamin D and molecular actions on the immune system: Modulation of innate and autoimmunity. J Mol Med (Berl) 2010;88:441-50.  Back to cited text no. 1
    
2.
Reis AF, Hauache OM, Velho G. Vitamin D endocrine system and the genetic susceptibility to diabetes, obesity and vascular disease. A review of evidence. Diabetes Metab 2005;31:318-25.  Back to cited text no. 2
    
3.
Wang TJ, Pencina MJ, Booth SL, Jacques PF, Ingelsson E, Lanier K, et al. Vitamin D deficiency and risk of cardiovascular disease. Circulation 2008;117:503-11.  Back to cited text no. 3
    
4.
Martini LA, Wood RJ. Vitamin D status and the metabolic syndrome. Nutr Rev 2006;64:479-86.  Back to cited text no. 4
    
5.
Krishnan AV, Trump DL, Johnson CS, Feldman D. The role of vitamin D in cancer prevention and treatment. Endocrinol Metab Clin North Am 2010;39:401-18.  Back to cited text no. 5
    
6.
Liu PT, Stenger S, Li H, Wenzel L, Tan BH, Krutzik SR, et al. Toll-like receptor triggering of a vitamin D-mediated human antimicrobial response. Science 2006;311:1770-3.  Back to cited text no. 6
    
7.
Holick MF. Vitamin D deficiency. N Engl J Med 2007;357:266-81.  Back to cited text no. 7
    
8.
Holick MF. The role of vitamin D for bone health and fracture prevention. Curr Osteoporos Rep 2006;4:96-102.  Back to cited text no. 8
    
9.
Isaia G, Giorgino R, Rini GB, Bevilacqua M, Maugeri D, Adami S. Prevalence of hypovitaminosis D in elderly women in Italy: Clinical consequences and risk factors. Osteoporos Int 2003;14:577-82.  Back to cited text no. 9
    
10.
Yang Z, Laillou A, Smith G, Schofield D, Moench-Pfanner R. A review of vitamin D fortification: Implications for nutrition programming in Southeast Asia. Food Nutr Bull 2013;34:S81-9.  Back to cited text no. 10
    
11.
Wagner D, Sidhom G, Whiting SJ, Rousseau D, Vieth R. The bioavailability of vitamin D from fortified cheeses and supplements is equivalent in adults. J Nutr 2008;138:1365-71.  Back to cited text no. 11
    
12.
Holick MF, Chen TC. Vitamin D deficiency: A worldwide problem with health consequences. Am J Clin Nutr 2008;87:1080S-6.  Back to cited text no. 12
    
13.
Biancuzzo RM, Young A, Bibuld D, Cai MH, Winter MR, Klein EK, et al. Fortification of orange juice with vitamin D (2) or vitamin D (3) is as effective as an oral supplement in maintaining vitamin D status in adults. Am J Clin Nutr 2010;91:1621-6.  Back to cited text no. 13
    
14.
Lu Z, Chen TC, Zhang A, Persons KS, Kohn N, Berkowitz R, et al. An evaluation of the vitamin D3 content in fish: Is the vitamin D content adequate to satisfy the dietary requirement for vitamin D? J Steroid Biochem Mol Biol 2007;103:642-4.  Back to cited text no. 14
    
15.
O'Donnell S, Cranney A, Horsley T, Weiler HA, Atkinson SA, Hanley DA, et al. Efficacy of food fortification on serum 25-hydroxyvitamin D concentrations: Systematic review. Am J Clin Nutr 2008;88:1528-34.  Back to cited text no. 15
    
16.
Ross AC, Manson JE, Abrams SA, Aloia JF, Brannon PM, Clinton SK, et al. The 2011 report on dietary reference intakes for calcium and vitamin D from the Institute of Medicine: What clinicians need to know. J Clin Endocrinol Metab 2011;96:53-8.  Back to cited text no. 16
    
17.
Greger JL. Dietary supplement use: Consumer characteristics and interests. J Nutr 2001;131:1339S-43.  Back to cited text no. 17
    
18.
Tripkovic L, Lambert H, Hart K, Smith CP, Bucca G, Penson S, et al. Comparison of vitamin D2 and vitamin D3 supplementation in raising serum 25-hydroxyvitamin D status: A systematic review and meta-analysis. Am J Clin Nutr 2012;95:1357-64.  Back to cited text no. 18
    
19.
O'Mahony L, Stepien M, Gibney MJ, Nugent AP, Brennan L. The potential role of vitamin D enhanced foods in improving vitamin D status. Nutrients 2011;3:1023-41.  Back to cited text no. 19
    
20.
Kazmi SA, Vieth R, Rousseau D. Vitamin D3 fortification and quantification in processed dairy products. Int Dairy J 2007;17:753-9.  Back to cited text no. 20
    
21.
Vieth R. Implications for 25-hydroxyvitamin D testing of public health policies about the benefits and risks of vitamin D fortification and supplementation. Scand J Clin Lab Invest Suppl 2012;243:144-53.  Back to cited text no. 21
    
22.
Natri AM, Salo P, Vikstedt T, Palssa A, Huttunen M, Kärkkäinen MU, et al. Bread fortified with cholecalciferol increases the serum 25-hydroxyvitamin D concentration in women as effectively as a cholecalciferol supplement. J Nutr 2006;136:123-7.  Back to cited text no. 22
    
23.
Tangpricha V, Koutkia P, Rieke SM, Chen TC, Perez AA, Holick MF. Fortification of orange juice with vitamin D: A novel approach for enhancing vitamin D nutritional health. Am J Clin Nutr 2003;77:1478-83.  Back to cited text no. 23
    
24.
Urbain P, Singler F, Ihorst G, Biesalski HK, Bertz H. Bioavailability of vitamin D2 from UV-B-irradiated button mushrooms in healthy adults deficient in serum 25-hydroxyvitamin D: A randomized controlled trial. Eur J Clin Nutr 2011;65:965-71.  Back to cited text no. 24
    
25.
Nikooyeh B, Neyestani TR, Farvid M, Alavi-Majd H, Houshiarrad A, Kalayi A, et al. Daily consumption of vitamin D- or vitamin D+calcium-fortified yogurt drink improved glycemic control in patients with type 2 diabetes: A randomized clinical trial. Am J Clin Nutr 2011;93:764-71.  Back to cited text no. 25
    
26.
Holmberg I, Aksnes L, Berlin T, Lindbäck B, Zemgals J, Lindeke B. Absorption of a pharmacological dose of vitamin D3 from two different lipid vehicles in man: Comparison of peanut oil and a medium chain triglyceride. Biopharm Drug Dispos 1990;11:807-15.  Back to cited text no. 26
    
27.
Hartman AM, Dryden LP. Vitamins in milk and milk products. In: Webb BH, Johnson AH, Alford JA, editors. Fundamentals of Dairy Chemistry. 2nd ed. New York: AVI/Van Nostrand Reinhold; 1974. p. 325-401.  Back to cited text no. 27
    
28.
Krauss WE, Erb JH, Washburn RG. Studies on the nutritive value of milk. II. The effect of pasteurization on some of the nutritive properties of milk. Ohio Agric Exp Stn Bull 1933;518:1-33.  Back to cited text no. 28
    
29.
Upreti P, Mistry VV, Warthesen JJ. Estimation and fortification of vitamin D3 in pasteurized process cheese. J Dairy Sci 2002;85:3173-81.  Back to cited text no. 29
    
30.
Renken SA, Warthesen JJ. Vitamin D stability in milk. J Food Sci 1993;58:552-5.  Back to cited text no. 30
    
31.
Gaylord AM, Warthesen JJ, Smith DE. Influence of milk fat, milk solids, and light intensity on the light stability of vitamin A and riboflavin in lowfat milk. J Dairy Sci 1986;69:2779-84.  Back to cited text no. 31
[PUBMED]    
Advanced Biomedical Research
Volume 2016, March
March 2016
Pages 1-6