Distribution of erm genes among Staphylococcus aureus isolates with inducible resistance to clindamycin in Isfahan, Iran

Ghanbari, Fahimeh; Ghajavand, Hasan; Havaei, Roholla; Jami, Mohammad-Saeid; Khademi, Farzad; Heydari, Leila; Shahin, Mojtaba; Havaei, Seyed Asghar

Distribution of erm genes among Staphylococcus aureus isolates with inducible resistance to clindamycin in Isfahan, Iran

Authors

¹ Department of Microbiology, Faculty of Bioscience, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

² Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

³ Department of Endodontics, School of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran

⁴ Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

⁵ Department of Medical Bacteriology and Virology, Ghaem Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

⁶ Department of Microbiology, School of Medicine, Ahvaz University of Medical Sciences, Ahvaz, Iran

Abstract

Background: The rising frequency of methicillin resistant Staphylococcus aureus (MRSA) has led to an increased use of antibiotics such as macrolide, lincosamide, streptogramin B (MLS_B) for the treatment of S. aureus infections. Resistance to MLS_Bin S. aureus is commonly encoded by erm genes, which can be constitutive MLS_B (cMLS_B) or inducible MLS_B (iMLS_B). The purpose of this study was to determine the frequency of cMLS_B, iMLS_B, and MS phenotypes using D-test and polymerase chain reaction (PCR) methods.
Materials and Methods: A total of 215 isolates of S. aureus were collected from January 2010 to May 2012 from Al-Zahra Hospital in Isfahan. PCR was performed for detection of mecA gene on all isolates using specific primers. The frequency of MLS_B-resistant isolates was determined using D-test, and then a multiplex PCR was performed for detection of ermA, ermB, and ermC genes.

Results: Among 215 S. aureus isolates examined, 82 (40.9%) were MRSA, and iMLS_B, cMLS_B, and MS resistance phenotypes had a frequency of 9 (4.18%), 58 (26.9%), and 11 (5.1%), respectively. Among nine isolates with iMLS_Bresistance phenotype, four isolates contained ermC gene, two isolates ermB gene, and one isolate ermA gene. Two isolates did not have any erm gene.
Conclusion: In the current study, cMLS_Bwas the most frequent phenotype and ermC was the most common gene in iMLS_Bresistant phenotypes.

Keywords

References

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