Anticarcinogenic and antimutagenic activity of Alstonia scholaris on the albino mice bone marrow cells and peripheral human lymphocyte culture against methyl methane sulfonate induced genotoxicity


1 Department of Zoology, Shibli National (PG) College, Azamgarh, Uttar Pradesh, India

2 Department of Zoology, D.S. College, Aligarh, Uttar Pradesh, India

3 Department of Zoology, Faculty of Life Science, Section of Genetics, Aligarh Muslim University, Aligarh, Uttar Pradesh, India


Background: The use of medicinal plants in modern medicine for the prevention and treatment of cancer is an important aspect. For this reason, it is important to identify antitumor promoting agents present in medicinal plants commonly used by the human population.
Materials and Methods: We used in vivo and in vitro methods using chromosomal aberrations (CAs), sister chromatid exchange (SCE) and replication index (RI) as markers, exposed by methyl methanesulfonate (MMS) as well as alcoholic extract of Alstonia scholaris in five increasing concentrations (200, 250, 300, 350 and 400 mg/kg body weight for in vivo and 150, 200, 250 and 300 μg/ml of culture) and of three different durations of 24, 48 and 72 h in the presence as well absence of S9mix.

Results: Extracts of Alstonia reduces the total aberrant cells ranges from 10.0% to 41.84% and frequencies of aberration in the aberrant cells ranges from 220 to 124 against 290 aberrations causes due to MMS in vivo. Similarly in the in vitro, it reduces CAs (39.62%, 32.83%, and 38.48%) and (45.31%, 44.46%, and 38.34%) at 24, 48, and 72 h of exposure respectively; in the absence as well as presence of liver S9fraction. It also reduces SCE from 7.70 to 4.20 per cell and enhances RI from 1.45 to 1.64.
Conclusion: Extracts of Alstonia significantly reduces the number of aberrant cells and frequency of aberration per cell at each concentration and duration of exposure in vivo; and CAs and SCE in vitro and enhances RI.


Balachandran P, Govindarajan R. Cancer – An ayurvedic perspective. Pharmacol Res 2005;51:19-30.  Back to cited text no. 1
Bradstreet K. Herbs for Detoxification. Australia: Woodland Publishing; 1997. p. 10-3.  Back to cited text no. 2
Nair CK, Divyasree P, Gopakumar G. Ethnomedicinal plants to fight neoplastic diseases. In: Debprasad C, editor. Ethnomedicine: A Source of Complementary Therapeutics. Trivandrum: Publisher Research Signpost; 2010. p. 661.  Back to cited text no. 3
Dey A. Alstonia scholaris R. Br. (Apocynaceae): Phytochemistry and pharmacology: A concise review. J Appl Pharm Sci 2011;01:51-7.  Back to cited text no. 4
Joshi SG. Medicinal Plants. New Delhi: Oxford and IBH Publishing Co. Pvt. Ltd.; 2000.  Back to cited text no. 5
Arulmozhi S, Mazumder PM, Sathiya NL, Prasad A, Thakurdesai P.In vitro antioxidant and free radical scavenging activity of fractions from Alstonia scholaris Linn. R. Br. Int J Pharm Technol Res 2010;2:18-25.  Back to cited text no. 6
Iwo MI, Soemardji AA, Retnoningrum DS, Sukrasno UUM. Immunostimulating effect of pule (Alstonia scholaris L. R. Br. Apocynaceae) bark extracts. Clin Hemorheol Microcirc 2000;23:177-83.  Back to cited text no. 7
Saraswathi V, Mathuram V, Subramanian S, Govindasamy S. Modulation of the impaired drug metabolism in sarcoma-180-bearing mice by echitamine chloride. Cancer Biochem Biophys 1999;17:79-88.  Back to cited text no. 8
Jagetia GC, Baliga MS. The evaluation of nitric oxide scavenging activity of certain Indian medicinal plants in vitro: A preliminary study. J Med Food 2004;7:343-8.  Back to cited text no. 9
Es-Safi NE, Khlifi S, Kerhoas L, Kollmann A, El Abbouyi A, Ducrot PH. Antioxidant constituents of the aerial parts of Globularia alypum growing in Morocco. J Nat Prod 2005;68:1293-6.  Back to cited text no. 10
Harish R, Shivanandappa T. Antioxidant activity and hepatoprotective potential of Phyllanthus niruri. Food Chem 2006;95:180-5.  Back to cited text no. 11
Channa S, Dar A, Ahmed S, Atta-ur-Rahman. Evaluation of Alstonia scholaris leaves for broncho-vasodilatory activity. J Ethnopharmacol 2005;97:469-76.  Back to cited text no. 12
Baliga MS. Alstonia scholaris Linn R Br in the treatment and prevention of cancer: Past, present, and future. Integr Cancer Ther 2010;9:261-9.  Back to cited text no. 13
Maron DM, Ames BN. Revised methods for the Salmonella mutagenicity test. Mutat Res 1983;113:173-215.  Back to cited text no. 14
Evans HJ. Human peripheral blood lymphocytes for the analysis of chromosome aberrations in mutagen tests. In: Kiley BJ, Legator M, Nichols W, Ramel C, editors. Handbook of Mutegenicity Test Procedures. Amsterdam: Elsevier; 1984. p. 40-427.  Back to cited text no. 15
Latt SA, Wohlleb JC. Optical studies of the interaction of 33258 Hoechst with DNA, chromatin, and metaphase chromosomes. Chromosoma 1975;52:297-316.  Back to cited text no. 16
Latt SA, Allen J, Bloom SE, Carrano A, Falke E, Kram D, et al. Sister-chromatid exchanges: A report of the GENE-TOX program. Mutat Res 1981;87:17-62.  Back to cited text no. 17
Crossen PE, Morgan WF. Analysis of human lymphocyte cell cycle time in culture measured by sister chromatid differential staining. Exp Cell Res 1977;104:453-7.  Back to cited text no. 18
Tice R, Schneider EL, Rary JM. The utilization of bromodeoxyuridine incorporation into DNA for the analysis of cellular kinetics. Exp Cell Res 1976;102:232-6.  Back to cited text no. 19
Fisher A, Yates F. Statistical Tables for Biological Agricultural and Medical Research. 6th ed. New York: Hafner Pub. Co.; 1963.  Back to cited text no. 20
Jagetia GC, Baliga MS. Evaluation of anticancer activity of the alkaloid fraction of Alstonia scholaris in vitro and in vivo. J Med Food 2006;20:103-9.  Back to cited text no. 21
Jahan S, Chaudhary R, Goyal PK. Anticancer activity of an Indian medicinal plant, Alstonia scholaris, on skin carcinogenesis in mice. Integr Cancer Ther 2009;8:273-9.  Back to cited text no. 22
Morse MA, Stoner GD. Cancer chemoprevention: Principles and prospects. Carcinogenesis 1993;14:1737-46.  Back to cited text no. 23
Stoner GD, Mukhtar H. Polyphenols as cancer chemopreventive agents. J Cell Biochem Suppl 1995;22:169-80.  Back to cited text no. 24
DiGiovanni J. Multistage carcinogenesis in mouse skin. Pharmacol Ther 1992;54:63-128.  Back to cited text no. 25
Jagetia GC, Baliga MS. Treatment with Alstonia scholaris enhances radiosensitivity in vitro and in vivo. J Med Food 2003;18:917-29.  Back to cited text no. 26
Swafiya J, Ranu C, Pradeepkumar G. Anticancer activity of an Indian medicinal plant, Alstonia scholaris on skin carcinogenesis in mice. Integr Cancer Ther 2010;9:261-9.  Back to cited text no. 27
Baliga MS, Jagetia GC, Ulloor JN, Baliga MP, Venkatesh P, Reddy R, et al. The evaluation of the acute toxicity and long term safety of hydroalcoholic extract of Sapthaparna (Alstonia scholaris) in mice and rats. Toxicol Lett 2004;151:317-26.  Back to cited text no. 28
Arulmozhi S, Rasal VP, Sathiyanarayanan L, Ashok P. Screening of Alstonia scholaris Linn. R Br. for wound healing activity. Orient Pharm Exp Med 2007;7:254-60.  Back to cited text no. 29
Mau JL, Chao GR, Wu KT. Antioxidant properties of methanolic extracts from several ear mushrooms. J Agric Food Chem 2001;49:5461-7.  Back to cited text no. 30
Rai S, Wahile A, Mukherjee K, Saha BP, Mukherjee PK. Antioxidant activity of Nelumbo nucifera (sacred lotus) seeds. J Ethnopharmacol 2006;104:322-7.  Back to cited text no. 31
Khan MR, Omoloso AD, Kihara M. Antibacterial activity of Alstonia scholaris and Leea tetramera. Fitoterapia 2003;74:736-40.  Back to cited text no. 32