Advanced Biomedical Research

Preparation and evaluation of a hair wax containing propolis and Eruca sativa seed oil for hair growth

Document Type : Original Article

Authors

1 Department of Pharmaceutics, Novel Drug Delivery Systems Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

2 Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan, Iran

3 Department of Clinical Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Hair growth as a key consumer objective has important role in the hair care products researches. This study was aimed to investigate the effect of a hair wax containing propolis, a resinous mixture produced by honeybees in Eruca sativa seed oil base on hair growth.
Materials and Methods: The hair wax was designed and formulated compared with marketed brand hair wax and evaluated for pharmaceutical parameters including pH, homogeneity, consistency, spread ability, in vitro drug release, and stability. After selection of the best formulation containing 10% ethanolic extract of propolis and 10% E. sativa seed oil, the hair growth potential was evaluated by application of 1 g hair wax daily on 4 cm2 area of dorsal side of Wistar rats and compared with controls and standard medication (1 ml of 2% minoxidil). After 30 days treatment, the length and weight of hairs and percentage of hair follicles in different phases of growth in skin biopsies were assessed.
Results: The selected hair wax formulation was stable and easy to wash. The formulation significantly increased hair length on 10th, 20th, and 30th day compared control group (5.8 ± 0.3 vs. 2.6 ± 0.4, 11.4 ± 0.6 vs. 5.8 ± 0.4, and 17.5 ± 0.5 vs. 12.7 ± 0.4 mm, respectively) and also the weight of newly grown hairs on 30th day (0.056 ± 0.006 vs. 0.043 ± 0.005). It improved hair follicles percentages in anagen phase without any sensitivity reaction.
Conclusions: The results of this study suggest that the formulated hair wax containing of propolis and E. sativa seed oil could have significant effect on promoting hair growth.

Keywords

1.
Kumar S, Wahid A, Anoop KV. Physiology and anatomy of hair in drug abusing cases. IJMTFM 2012;2:153-9.  Back to cited text no. 1
    
2.
McElwee JK, Sinclair R. Hair physiology and its disorders. Drug Discov Today Dis Mech 2008;5:e163–e171.  Back to cited text no. 2
    
3.
Franca K, Rodrigues TS, Ledon J, Savas J, Chacon A. Comprehensive overview and treatment update on hair loss. J Cosmet Dermatol Sci Appl 2013;3:1-8.  Back to cited text no. 3
    
4.
Upadhyay S, Ghosh AK, Singh V. Hair growth promotant activity of petroleum ether extract of Glycyrrhiza Glabra L (Fabaceae) in female rats. Trop J Pharm Res 2012;11:753-8.  Back to cited text no. 4
    
5.
Marcucci MC. Propolis: Chemical composition, biological properties and therapeutic activity. Apidologie 1995;26:83-99.  Back to cited text no. 5
    
6.
Yaghoubi SM, Ghorbani GR, Soleimanian-Zade S, Satari R. Antimicrobial activity of Iranian propolis and its chemical composition. Daru 2007;15:45-8.  Back to cited text no. 6
    
7.
Rebiai A, Lanez T, Belfar ML. Total polyphenol contents, radical scavenging and cyclic voltammetry of Algerian propolis. Int J Pharm Pharm Sci 2014;6:395-400.  Back to cited text no. 7
    
8.
Jastrzebska-Stojko Z, Stojko R, Rzepecka-Stojko A, Kabala-Dzik A, Stojko J. Biological activity of propolis-honey balm in the treatment of experimentally-evoked burn wounds. Molecules 2013;18:14397-413.  Back to cited text no. 8
    
9.
Miyata S, Oda Y, Matsuo C, Kumura H, Kobayashi K. Stimulatory effect of Brazilian propolis on hair growth through proliferation of keratinocytes in mice. J Agric Food Chem 2014;62:11854-61.  Back to cited text no. 9
    
10.
Sharma V, Garg G, Alam A. Extraction and characterization of industrially valuable oil from Eruca sativa (L.) Mill. through FT-IR and GC-MS analysis. Am J Biol Chem 2014;2:23-8.  Back to cited text no. 10
    
11.
Sadiq A, Hayat MQ, Mall SM. Qualitative and quantitative determination of secondary metabolites and antioxidant potential of Eruca sativa. Nat Prod Chem Res 2014;2:1-7.  Back to cited text no. 11
    
12.
Cavaiuolo M, Ferrante A. Nitrates and glucosinolates as strong determinants of the nutritional quality in rocket leafy salads. Nutrients 2014;6:1519-38.  Back to cited text no. 12
    
13.
Ghorbani A. Studies on pharmaceutical ethnobotany in the region of Turkmen Sahra, north of Iran (Part 1): General results. J Ethnopharmacol 2005;102:58-68.  Back to cited text no. 13
    
14.
Mikaili P, Shayegh J, Sarahroodi SH, Sharifi M. Pharmacological properties of herbal oil extracts used in Iranian traditional medicine. Adv Environ Biol 2012;6:153-8.  Back to cited text no. 14
    
15.
Rani I, Akhund SH, Suhail M, Abro H. Antimicrobial potential of seed extract of Eruca sativa. Pak J Bot 2010;42:2949-53.  Back to cited text no. 15
    
16.
Rajaei P, Mohamadi N. Ethnobotanical study of medicinal plants of hezar mountain allocated in South East of Iran. Iran J Pharm Res 2012;11:1153-67.  Back to cited text no. 16
    
17.
Coneac G, Gafiţanu E, Hadaruga DI, Hadaruga NG, Pinzaru IN, Bandur G, et al. Flavonoid contents of propolis from the west side of Romania and correlation with the antioxidant activity. Chem Bull Politehnica Univ 2008;53:1-2.  Back to cited text no. 17
    
18.
Waterhouse AL. Current Protocols in Food Analytical Chemistry. Unit. I, Vol. 1. New York: John Wiley and Sons; 2002. p. 1-8.  Back to cited text no. 18
    
19.
Association of Official Analytical Chemists. Official Methods of Analysis. 17th ed. Washington, DC: Association of Official Analytical Chemists; 1997.  Back to cited text no. 19
    
20.
Zhang Q, Wu J, Ma P, Cai J, Zhang Y. Acid value determination and pre-esterification of crude Euphorbia lathyris L. Oil. World J Eng Technol 2015;3:70-5.  Back to cited text no. 20
    
21.
Gidwani B, Alaspure RN, Duragkar NJ, Singh V, Rao SP, Shukla SS. Evaluation of a novel herbal formulation in the treatment of eczema with Psoralea corylifolia. Iran J Dermatol 2010;13:122-7.  Back to cited text no. 21
    
22.
Kumar KK, Sasikanth K, Sabareesh M, Dorababu N. Formulation and evaluation of diacerein cream. Asian J Pharm Clin Res 2011;4:93-8.  Back to cited text no. 22
    
23.
George E, Mathews MM. Formulation and evaluation of topical gel containing hair growth promoters for the treatment of androgenic alopecia. Bull Pharm Res 2014;4:1-8.  Back to cited text no. 23
    
24.
Aslani A, Ghannadi A, Najafi H. Design, formulation and evaluation of a mucoadhesive gel from Quercus brantii L. and Coriandrum sativum L. As periodontal drug delivery. Adv Biomed Res 2013;2:21.  Back to cited text no. 24
    
25.
Allayie SA, Hemalatha S, Elanchezhiyan C, Manoharan V, Balasubramanian K, Sheikh BA.In vivo evaluation of hair growth potential of fresh leaf extracts of Naringi crenulata. J Clin Exp Dermatol Res 2012;3:1-4.  Back to cited text no. 25
    
26.
Builders PF, Iwu IW, Mbah CC, Iwu IW, Builders MI, Audu MM. Moringa oleifera ethosomes a potential hair growth activator: Effect on rats. J Pharm Biomed Sci 2014;4:611-8.  Back to cited text no. 26
    
27.
Hartwig S, Auwärter V, Pragst F. Effect of hair care and hair cosmetics on the concentrations of fatty acid ethyl esters in hair as markers of chronically elevated alcohol consumption. Forensic Sci Int 2003;131:90-7.  Back to cited text no. 27
    
28.
Kakali D, Anu TS, Ashok M, Beena B, Ramesh B, Anand CB. Eclipta alba extract with potential for hair growth promoting activity. J Ethnopharmacol 2009;124:450-6.  Back to cited text no. 28
    
29.
DeVillez RL. The therapeutic use of topical minoxidil. Dermatol Clin 1990;8:367-75.  Back to cited text no. 29
    
30.
Suraja R, Rejitha G, Sunilson JA, Anandarajagopal K, Promwichita P.In vivo hair growth activity of Prunus dulcis seeds in rats. Biol Med 2009;1:34-8.  Back to cited text no. 30
    
31.
McClellan KJ, Markham A. Finasteride: A review of its use in male pattern hair loss. Drugs 1999;57:111-26.  Back to cited text no. 31
    
32.
Banerjee PS, Sharma M, Nema RK. Preparation, evaluation and hair growth stimulating activity of herbal hair oil. JOCPR 2009;1:261-7.  Back to cited text no. 32
    
33.
Philpott MP, Green MR, Kealey T. Rat hair follicle growth in vitro. Br J Dermatol 1992;127:600-7.  Back to cited text no. 33
    
34.
Shimizu K, Kondo R, Sakai K, Shoyama Y, Sato H, Ueno T. Steroid 5alpha-reductase inhibitory activity and hair regrowth effects of an extract from Boehmeria nipononivea. Biosci Biotechnol Biochem 2000;64:875-7.  Back to cited text no. 34
    
35.
Ramanauskiene K, Inkeniene AM, Petrikaite V, Briedis V. Total phenolic content and antimicrobial activity of different Lithuanian propolis solutions. Evid Based Complement Alternat Med 2013;2013:1-5.  Back to cited text no. 35
    
36.
Cruz ST, Estrada GP, Lopez ZC, Martinez MA, Valencia VP, Orozco AL. Use of propolis for topical treatment of dermatophytosis in dog. Open J Vet Med 2014;4:239-45.  Back to cited text no. 36
    
37.
Djuric N, Djuric S. Use of four honeybee products to treat alopecia (hair loss). J Am Apitherapy Soc 2010;17:4-5.  Back to cited text no. 37
Advanced Biomedical Research
Volume 2016, November
November 2016
Pages 1-7