Comparison of the Frequency of Y-short Tandem Repeats Markers between Sadat and Non-Sadat Populations in Isfahan Province of Iran

Seyedebrahimi, Reihaneh; Esfandiari, Ebrahim; Rashidi, Bahman; Salehi, Rasoul; Dahghi, Ali Gholami; Dabiri, Shahriar; Kheirollahi, Majid

Comparison of the Frequency of Y-short Tandem Repeats Markers between Sadat and Non-Sadat Populations in Isfahan Province of Iran

Document Type : Original Article

Authors

¹ Department of Anatomy, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

² Department of Genetics and Molecular Biology; Pediatric Inherited Diseases Research Center, Research Institute for Primordial Prevention of Noncommunicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran

³ Department of Islamic Studies, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

⁴ Department of Pathology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

Abstract

Background: Y chromosome is one of the two sex chromosomes and is male specific. Due to limited genetic exchange, the main part of that is passed virtually unchanged from one generation to next generation. The short tandem repeats (STRs) are almost constant on chromosomes that make them as an appropriate factor for use in population genetic studies. In this study, we used the STRs of Y chromosome markers in Sadat families and comparison with other families was investigated. Materials and Methods: In this study, sampling was done from fifty unrelated males of Sadat families and fifty unrelated males of non-Sadat families. After the extraction of DNA from blood samples and primer design, polymerase chain reaction (PCR) was performed for each primer pairs separately. The PCR products were run on agarose gel that followed by running on polyacrylamide gel for better resolution. In addition, some sequenced samples were used as identified markers to determine the length of other alleles in polyacrylamide gel. Results: The survey of six STR in two case and control groups was carried out, and analysis revealed that the frequency of some alleles is different in case group compared to control group. Allele frequency of the markers DYS392, DYS393, DYS19, DYS390, DYS388, and DYS437 on the Y chromosome in Sadat families was quite different in comparison with other families. Conclusions: The reason for these differences in allele frequencies of the Sadat family in comparison with other families is having a common ancestor.

Keywords

References

1.	Gusmão L, Carracedo A. Y Chromosome-Specifi c STRs. Profi les in DNA; 2003. Available from: http://www.bioplein.nl/attachments/File/Humane_Sporen/Profi lesInDNA_601_03.pdf. [Last accessed on 2016 Jun 14].
2.	Just W, Baumstark A, Süss A, Graphodatsky A, Rens W, Schäfer N, et al. Ellobius lutescens: Sex determination and sex chromosome. Sex Dev 2007;1:211-21.
3.	Kwak KD, Jin HJ, Shin DJ, Kim JM, Roewer L, Krawczak M, et al. Y-chromosomal STR haplotypes and their applications to forensic and population studies in East Asia. Int J Legal Med 2005;119:195-201.
4.	Underhill PA, Kivisild T. Use of Y chromosome and mitochondrial DNA population structure in tracing human migrations. Annu Rev Genet 2007;41:539-64.
5.	Quintana-Murci L, Krausz C, McElreavey K. The human Y chromosome: Function, evolution and disease. Forensic Sci Int 2001;118:169-81.
6.	Iida R, Kishi K. Identification, characterization and forensic application of novel Y-STRs. Leg Med (Tokyo) 2005;7:255-8.
7.	Butler JM. Short tandem repeat typing technologies used in human identity testing. Biotechniques 2007;43:ii-v.
8.	Bentayebi K, Abada F, Ihzmad H, Amzazi S. Genetic ancestry of a Moroccan population as inferred from autosomal STRs. Meta Gene 2014;2:427-38.
9.	Nayak BP, Khajuria H, Gupta S. Genetic analysis of Y-chromosomal STRs in Khandayat population of Odisha, INDIA. Int Res J Biol Sci 2014;3:26-8.
10.	Roewer L, Kayser M, Dieltjes P, Nagy M, Bakker E, Krawczak M, et al. Analysis of molecular variance (AMOVA) of Y-chromosome-specific microsatellites in two closely related human populations. Hum Mol Genet 1996;5:1029-33.
11.	Xue F, Wang Y, Xu S, Zhang F, Wen B, Wu X, et al. A spatial analysis of genetic structure of human populations in China reveals distinct difference between maternal and paternal lineages. Eur J Hum Genet 2008;16:705-17.
12.	Farazmand A, Sokhansanj A, Rafee MR, Mehrabani Y. Comparative analysis of Y-chromosomal short tandem repeats (YSTRs) polymorphism in an Iranian Sadat subpopulation. JSUT 2009;35:7-12.
13.	Ploski R, Wozniak M, Pawlowski R, Monies DM, Branicki W, Kupiec T, et al. Homogeneity and distinctiveness of Polish paternal lineages revealed by Y chromosome microsatellite haplotype analysis. Hum Genet 2002;110:592-600.
14.	Valverde L, Rosique M, Köhnemann S, Cardoso S, García A, Odriozola A, et al. Y-STR variation in the Basque diaspora in the Western USA: Evolutionary and forensic perspectives. Int J Legal Med 2012;126:293-8.
15.	Moorjani P, Patterson N, Hirschhorn JN, Keinan A, Hao L, Atzmon G, et al. The history of African gene flow into Southern Europeans, Levantines, and Jews. PLoS Genet 2011;7:e1001373.
16.	Serin A, Canan H, Alper B, Sertdemir Y. Haplotype frequencies of 17 Y-chromosomal short tandem repeat loci from the Cukurova region of Turkey. Croat Med J 2011;52:703-8.
17.	Sun H, Zhou C, Huang X, Lin K, Shi L, Yu L, et al. Autosomal STRs provide genetic evidence for the hypothesis that Tai people originate from Southern China. PLoS One 2013;8:e60822.

Advanced Biomedical Research