Polymorphism (rs16917496) at the miR-502 Binding Site of the Lysine Methyltransferase 5A (SET8) and its Correlation with Colorectal Cancer in Iranians

Document Type : Original Article

Authors
Meysam Mosallayi 1
Miganoosh Simonian 1
Sharifeh Khosravi 1
Ahmad Reza Salehi 1
Mahsa Khodadoostan 2
Vahid Sebghatollahi 2
Azar Baradaran 3
Rasoul Salehi 4
1 Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Department of Internal Medicine, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4 Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan; Acquired Immunodeficiency Research Center, Isfahan University of Medical Sciences, Isfahan; Gerfa Namayesh Azmayesh (GENAZMA) Science and Research Institute, Isfahan, Iran
Abstract
Background: One of the gene expression regulatory mechanisms is mediated by small noncoding RNAs called microRNA (miRNA). They interact with a recognition sequence located mostly in 3'-untranslated regions (3'-UTRs) of mRNAs. Polymorphisms in miRNAs recognition sequences could affect gene expression which in turn may alter disease susceptibility. SET8, a member of the SET domain-containing methyltransferase, acts in a variety of biological processes such as genomic stability. Here, we report correlation of rs16917496 polymorphism, located in the recognition sequence of miR-502 within 3'-UTR of SET8, with colorectal cancer (CRC) in Iranians. Materials and Methods: One hundred and seventy CRC patients and 170 noncancer counterparts were recruited in this case–control study. Genotyping of rs16917496 was performed using polymerase chain reaction-restriction fragment length polymorphism method. Results: There was no significant association of rs16917496 with CRC in population under study (P value for genotype and allele distribution were >0.05). However, stratification analysis based on smoking status revealed that TT+TC genotypes of SET8 rs16917496 are strongly associated with increased risk of CRC (odds ratio: 5.8, 95% confidence interval: 1.37–24.34, P - 0.005) in smoker subgroup. Conclusion: Correlation of rs16917496 T allele with CRC in smokers is emphasizing the importance of individuals' genotype in the recruitment of adverse health hazards of smoking more profoundly for certain people compared to others.

Keywords

1.
Huang SH, Chao Y, Wu YY, Luo JC, Kao CH, Yen SH, et al. Concurrence of UGT1A polymorphism and end-stage renal disease leads to severe toxicities of irinotecan in a patient with metastatic colon cancer. Tumori 2011;97:243-7.  Back to cited text no. 1
[PUBMED]    
2.
Chae YS, Kim JG, Lee SJ, Kang BW, Lee YJ, Park JY, et al. A miR-146a polymorphism (rs2910164) predicts risk of and survival from colorectal cancer. Anticancer Res 2013;33:3233-9.  Back to cited text no. 2
[PUBMED]    
3.
Saito R, Suzuki H, Yamada T, Endo S, Moriwaki T, Ueno T, et al. Predicting skin toxicity according to EGFR polymorphisms in patients with colorectal cancer receiving antibody against EGFR. Anticancer Res 2013;33:4995-8.  Back to cited text no. 3
[PUBMED]    
4.
Bhaumik P, Gopalakrishnan C, Kamaraj B, Purohit R. Single nucleotide polymorphisms in microRNA binding sites: Implications in colorectal cancer. ScientificWorldJournal 2014;2014:547154.  Back to cited text no. 4
[PUBMED]    
5.
Feik E, Baierl A, Hieger B, Führlinger G, Pentz A, Stättner S, et al. Association of IGF1 and IGFBP3 polymorphisms with colorectal polyps and colorectal cancer risk. Cancer Causes Control 2010;21:91-7.  Back to cited text no. 5
    
6.
Kolahdoozan S, Sadjadi A, Radmard AR, Khademi H. Five common cancers in Iran. Arch Iran Med 2010;13:143-6.  Back to cited text no. 6
[PUBMED]    
7.
Hosseini SV, Izadpanah A, Yarmohammadi H. Epidemiological changes in colorectal cancer in Shiraz, Iran: 1980-2000. ANZ J Surg 2004;74:547-9.  Back to cited text no. 7
[PUBMED]    
8.
Chang WS, Yueh TC, Tsai CW, Ji HX, Wu CN, Wang SC, et al. Contribution of DNA repair xeroderma pigmentosum group D Genotypes to colorectal cancer risk in Taiwan. Anticancer Res 2016;36:1657-63.  Back to cited text no. 8
    
9.
Tomlinson I, Webb E, Carvajal-Carmona L, Broderick P, Kemp Z, Spain S, et al. A genome-wide association scan of tag SNPs identifies a susceptibility variant for colorectal cancer at 8q24.21. Nat Genet 2007;39:984-8.  Back to cited text no. 9
[PUBMED]    
10.
Tenesa A, Farrington SM, Prendergast JG, Porteous ME, Walker M, Haq N, et al. Genome-wide association scan identifies a colorectal cancer susceptibility locus on 11q23 and replicates risk loci at 8q24 and 18q21. Nat Genet 2008;40:631-7.  Back to cited text no. 10
    
11.
Zhang K, Civan J, Mukherjee S, Patel F, Yang H. Genetic variations in colorectal cancer risk and clinical outcome. World J Gastroenterol 2014;20:4167-77.  Back to cited text no. 11
    
12.
Daraei A, Salehi R, Salehi M, Emami MH, Janghorbani M, Mohamadhashem F, et al. Effect of rs6983267 polymorphism in the 8q24 region and rs4444903 polymorphism in EGF gene on the risk of sporadic colorectal cancer in Iranian population. Med Oncol 2012;29:1044-9.  Back to cited text no. 12
[PUBMED]    
13.
Wu CZ, Ni XJ, Zheng SL, Yang YR, Xia P, Zeng YJ, et al. A fast SSP-PCR method for genotyping the ATP-binding cassette subfamily B member 1 gene C3435T and G2677T polymorphisms in Chinese transplant recipients. Tumori 2009;95:338-42.  Back to cited text no. 13
[PUBMED]    
14.
Landi D, Gemignani F, Naccarati A, Pardini B, Vodicka P, Vodickova L, et al. Polymorphisms within micro-RNA-binding sites and risk of sporadic colorectal cancer. Carcinogenesis 2008;29:579-84.  Back to cited text no. 14
[PUBMED]    
15.
Manikandan M, Munirajan AK. Single nucleotide polymorphisms in microRNA binding sites of oncogenes: Implications in cancer and pharmacogenomics. OMICS 2014;18:142-54.  Back to cited text no. 15
[PUBMED]    
16.
Ahangari F, Salehi R, Salehi M, Khanahmad H. A miRNA-binding site single nucleotide polymorphism in the 3'-UTR region of the NOD2 gene is associated with colorectal cancer. Med Oncol 2014;31:173.  Back to cited text no. 16
[PUBMED]    
17.
Vaishnavi V, Manikandan M, Munirajan AK. Mining the 3'UTR of autism-implicated genes for SNPs perturbing microRNA regulation. Genomics Proteomics Bioinformatics 2014;12:92-104.  Back to cited text no. 17
    
18.
Liu Z, Wei S, Ma H, Zhao M, Myers JN, Weber RS, et al. A functional variant at the miR-184 binding site in TNFAIP2 and risk of squamous cell carcinoma of the head and neck. Carcinogenesis 2011;32:1668-74.  Back to cited text no. 18
    
19.
Hashemi M, Sheybani-Nasab M, Naderi M, Roodbari F, Taheri M. Association of functional polymorphism at the miR-502-binding site in the 3' untranslated region of the SETD8 gene with risk of childhood acute lymphoblastic leukemia, a preliminary report. Tumour Biol 2014;35:10375-9.  Back to cited text no. 19
    
20.
Guo Z, Wu C, Wang X, Wang C, Zhang R, Shan B. A polymorphism at the miR-502 binding site in the 3'-untranslated region of the histone methyltransferase SET8 is associated with hepatocellular carcinoma outcome. Int J Cancer 2012;131:1318-22.  Back to cited text no. 20
    
21.
Diao L, Su H, Wei G, Li T, Gao Y, Zhao G, et al. Prognostic value of microRNA 502 binding site SNP in the 3'-untranslated region of the SET8 gene in patients with non-Hodgkin's lymphoma. Tumori 2014;100:553-8.  Back to cited text no. 21
    
22.
Shi X, Kachirskaia I, Yamaguchi H, West LE, Wen H, Wang EW, et al. Modulation of p53 function by SET8-mediated methylation at lysine 382. Mol Cell 2007;27:636-46.  Back to cited text no. 22
    
23.
Fang J, Feng Q, Ketel CS, Wang H, Cao R, Xia L, et al. Purification and functional characterization of SET8, a nucleosomal histone H4-lysine 20-specific methyltransferase. Curr Biol 2002;12:1086-99.  Back to cited text no. 23
    
24.
Julien E, Herr W. A switch in mitotic histone H4 lysine 20 methylation status is linked to M phase defects upon loss of HCF-1. Mol Cell 2004;14:713-25.  Back to cited text no. 24
    
25.
Tardat M, Brustel J, Kirsh O, Lefevbre C, Callanan M, Sardet C, et al. The histone H4 Lys 20 methyltransferase PR-Set7 regulates replication origins in mammalian cells. Nat Cell Biol 2010;12:1086-93.  Back to cited text no. 25
    
26.
Jørgensen S, Elvers I, Trelle MB, Menzel T, Eskildsen M, Jensen ON, et al. The histone methyltransferase SET8 is required for S-phase progression. J Cell Biol 2007;179:1337-45.  Back to cited text no. 26
    
27.
Xu J, Yin Z, Gao W, Liu L, Yin Y, Liu P, et al. Genetic variation in a microRNA-502 minding site in SET8 gene confers clinical outcome of non-small cell lung cancer in a Chinese population. PLoS One 2013;8:e77024.  Back to cited text no. 27
    
28.
Song F, Zheng H, Liu B, Wei S, Dai H, Zhang L, et al. An miR-502-binding site single-nucleotide polymorphism in the 3'-untranslated region of the SET8 gene is associated with early age of breast cancer onset. Clin Cancer Res 2009;15:6292-300.  Back to cited text no. 28
    
29.
Yang S, Guo H, Wei B, Zhu S, Cai Y, Jiang P, et al. Association of miR-502-binding site single nucleotide polymorphism in the 3'-untranslated region of SET8 and TP53 codon 72 polymorphism with non-small cell lung cancer in Chinese population. Acta Biochim Biophys Sin (Shanghai) 2014;46:149-54.  Back to cited text no. 29
    
30.
Yang S, Jiang P, Tang J. Association of miR-502-binding site single nucleotide polymorphism of SET8 gene with non-small cell lung cancer risk in Chinese people. J Bionanoscience 2013;7:585-9.  Back to cited text no. 30
    
31.
Yu Z, Li Z, Jolicoeur N, Zhang L, Fortin Y, Wang E, et al. Aberrant allele frequencies of the SNPs located in microRNA target sites are potentially associated with human cancers. Nucleic Acids Res 2007;35:4535-41.  Back to cited text no. 31
    
32.
Mi Y, Wang L, Zong L, Pei M, Lu Q, Huang P. Genetic variants in microRNA target sites of 37 selected cancer-related genes and the risk of cervical cancer. PLoS One 2014;9:e86061.  Back to cited text no. 32
    
33.
Naccarati A, Pardini B, Stefano L, Landi D, Slyskova J, Novotny J, et al. Polymorphisms in miRNA-binding sites of nucleotide excision repair genes and colorectal cancer risk. Carcinogenesis 2012;33:1346-51.  Back to cited text no. 33
    
34.
Nishioka K, Rice JC, Sarma K, Erdjument-Bromage H, Werner J, Wang Y, et al. PR-Set7 is a nucleosome-specific methyltransferase that modifies lysine 20 of histone H4 and is associated with silent chromatin. Mol Cell 2002;9:1201-13.  Back to cited text no. 34
    
35.
Ding C, Li R, Peng J, Li S, Guo Z. A polymorphism at the miR-502 binding site in the 3' untranslated region of the SET8 gene is associated with the outcome of small-cell lung cancer. Exp Ther Med 2012;3:689-92.  Back to cited text no. 35
    
36.
Wang C, Guo Z, Wu C, Li Y, Kang S. A polymorphism at the miR-502 binding site in the 3' untranslated region of the SET8 gene is associated with the risk of epithelial ovarian cancer. Cancer Genet 2012;205:373-6.  Back to cited text no. 36
    
37.
Wang Z, Dai X, Zhong J, Inuzuka H, Wan L, Li X, et al. SCF (ß-TRCP) promotes cell growth by targeting PR-Set7/Set8 for degradation. Nat Commun 2015;6:10185.  Back to cited text no. 37
Advanced Biomedical Research
Volume 2017, july
July 2017
Pages 1-7