Detection of Panton-valentine Leukocidin Gene Isoforms of Staphylococcus aureus Isolates in Al-Zahra Hospital, Isfahan-Iran

Document Type : Original Article


1 Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

2 Department of Endodontics, School of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran


Background: Panton-Valentine leukocidin (PVL) is a gamma-toxin produced by Staphylococcus aureus encoded by genes lukS/lukF-PV with several single-nucleotide polymorphisms. A mutation at nucleotide position 527 results in substitution of histidine (H) to arginine (R) at amino acid 176. The groups defined based on the amino acid change, the “R isoform” group and the “H isoform” group. The purpose of this study was to determine the frequency of PVL gene isoforms in S. aureus strains isolated from patients at Al-Zahra Hospital Isfahan and molecular characterization of PVL-positive methicillin-resistant S. aureus(MRSA) strains including the detection of mecA gene and staphylococcal chromosomal cassette mec (SCCmec) typing. Materials and Methods: In this study, 130 isolates of S. aureus were collected from Al-Zahra Hospital. The PVL gene identified using polymerase chain reaction (PCR); PCR products were sequenced to identify the type of isoform. The molecular characterization of isolates of PVL-positive MRSA including detection of mecA gene by PCR and also SCCmec typing was performed by multiplex PCR. Results: Out of 130 isolates, 23% were positive for the presence of PVL genes. The PVL positive isolates were comprised 37% (11/30) of methicillin-resistant isolates and 63% (19/30) of methicillin-susceptible S. aureus (MSSA) isolates. The results showed that 17 isolated carrying isoform H and 13 isolated carrying the R isoform. Conclusion: The PVL gene was predominantly found in MSSA isolates. There was no relation between PVL isoforms and the presence of mecA and SCCmec types.


Cupane L, Pugacova N, Berzina D, Cauce V, Gardovska D, Miklaševics E. Patients with Panton-Valentine leukocidin positive Staphylococcus aureus infections run an increased risk of longer hospitalisation. Int J Mol Epidemiol Genet 2012;3:48-55.  Back to cited text no. 1
Colin DA, Mazurier I, Sire S, Finck-Barbançon V. Interaction of the two components of leukocidin from Staphylococcus aureus with human polymorphonuclear leukocyte membranes: Sequential binding and subsequent activation. Infect Immun 1994;62:3184-8.  Back to cited text no. 2
Mariem BJ, Ito T, Zhang M, Jin J, Li S, Ilhem BB, et al. Molecular characterization of methicillin-resistant Panton-Valentine leukocidin positive Staphylococcus aureus clones disseminating in Tunisian hospitals and in the community. BMC Microbiol 2013;13:2.  Back to cited text no. 3
O'Hara FP, Guex N, Word JM, Miller LA, Becker JA, Walsh SL, et al. A geographic variant of the Staphylococcus aureus Panton-Valentine leukocidin toxin and the origin of community-associated methicillin-resistant S. aureus USA300. J Infect Dis 2008;197:187-94.  Back to cited text no. 4
Khosravi AD, Hoveizavi H, Farshadzadeh Z. The prevalence of genes encoding leukocidins in Staphylococcus aureus strains resistant and sensitive to methicillin isolated from burn patients in Taleghani hospital, Ahvaz, Iran. Burns 2012;38:247-51.  Back to cited text no. 5
Lina G, Piémont Y, Godail-Gamot F, Bes M, Peter MO, Gauduchon V, et al. Involvement of Panton-Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clin Infect Dis 1999;29:1128-32.  Back to cited text no. 6
Vandenesch F, Naimi T, Enright MC, Lina G, Nimmo GR, Heffernan H, et al. Community-acquired methicillin-resistant Staphylococcus aureus carrying Panton-Valentine leukocidin genes: Worldwide emergence. Emerg Infect Dis 2003;9:978-84.  Back to cited text no. 7
Besseyre des Horts T, Dumitrescu O, Badiou C, Thomas D, Benito Y, Etienne J, et al. A histidine-to-arginine substitution in Panton-Valentine leukocidin from USA300 community-acquired methicillin-resistant Staphylococcus aureus does not impair its leukotoxicity. Infect Immun 2010;78:260-4.  Back to cited text no. 8
Khokhlova O, Tomita Y, Hung WC, Takano T, Iwao Y, Higuchi W, et al. Elderly infection in the community due to ST5/SCCmecII methicillin-resistant Staphylococcus aureus(the New York/Japan clone) in Japan: Panton-Valentine leukocidin-negative necrotizing pneumonia. J Microbiol Immunol Infect 2015;48:335-9.  Back to cited text no. 9
Otokunefor K, Sloan T, Kearns AM, James R. Molecular characterization and Panton-Valentine leucocidin typing of community-acquired methicillin-sensitive Staphylococcus aureus clinical isolates. J Clin Microbiol 2012;50:3069-72.  Back to cited text no. 10
Tong SY, Lilliebridge RA, Holt DC, Coombs GW, Currie BJ, Giffard PM. Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis. Diagn Microbiol Infect Dis 2010;67:399-401.  Back to cited text no. 11
Dumitrescu O, Tristan A, Meugnier H, Bes M, Gouy M, Etienne J, et al. Polymorphism of the Staphylococcus aureus Panton-Valentine leukocidin genes and its possible link with the fitness of community-associated methicillin-resistant S. aureus. J Infect Dis 2008;198:792-4.  Back to cited text no. 12
Brown ML, O'Hara FP, Close NM, Mera RM, Miller LA, Suaya JA, et al. Prevalence and sequence variation of Panton-Valentine leukocidin in methicillin-resistant and methicillin-susceptible Staphylococcus aureus strains in the United States. J Clin Microbiol 2012;50:86-90.  Back to cited text no. 13
Tong SY, Lilliebridge RA, Bishop EJ, Cheng AC, Holt DC, McDonald MI, et al. Clinical correlates of Panton-Valentine leukocidin (PVL), PVL isoforms, and clonal complex in the Staphylococcus aureus population of Northern Australia. J Infect Dis 2010;202:760-9.  Back to cited text no. 14
Forbes BA, Sahm DF, Welssfeld AS. Bailey and Scott's Diagnostic Microbiology. 11th ed. St. Louis: Mosby Inc.; 2002. p. 285-92.  Back to cited text no. 15
Darabi N, Habibollahi H, Shahbabian K. Molecular Epidemiology of Staphylococcus aureus Isolated from patients and personnel in Army hospital. Ann Mil Health Sci Res. 2010; 8:193-199.  Back to cited text no. 16
Omar NY, Ali HA, Harfoush RA, El Khayat EH. Molecular typing of methicillin resistant Staphylococcus aureus clinical isolates on the basis of protein A and coagulase gene polymorphisms. Int J Microbiol 2014; 650328.  Back to cited text no. 17
Azimian A, Havaei SA, Fazeli H, Naderi M, Ghazvini K, Samiee SM, Soleimani M, Peerayeh SN. Genetic characterization of a vancomycin-resistant Staphylococcus aureus isolate from the respiratory tract of a patient in a university hospital in northeastern Iran. J Clin Microbiol 2012;50:3581-5.  Back to cited text no. 18
Molla-abbaszadeh H, Mobayen H, Mirzaei H. Identification of Panton Valentine Leukocidin (pvl) Genes in Staphylococcus aureusisolated from In- patients of Emam Reza and Shohada Hospitals of Tabriz by Real-Time PCR. Iran J Med Microbiol. 2013; 6:72-80.  Back to cited text no. 19
Ohadian Moghadam S, Havaei SA, Pourmand MR. Prevalence of methicillin-resistant Staphylococcus aureus carrying Panton-Valentine leukocidin gene in cutaneous infections in the city of Isfahan. J Med Bacteriol 2012;1:9-16.  Back to cited text no. 20
Nickerson EK, Wuthiekanun V, Wongsuvan G, Limmathurosakul D, Srisamang P, Mahavanakul W, et al. Factors predicting and reducing mortality in patients with invasive Staphylococcus aureus disease in a developing country. PLoS One 2009;4:e6512.  Back to cited text no. 21
Chini V, Petinaki E, Foka A, Paratiras S, Dimitracopoulos G, Spiliopoulou I. Spread of Staphylococcus aureus clinical isolates carrying Panton-Valentine leukocidin genes during a 3-year period in Greece. Clin Microbiol Infect 2006;12:29-34.  Back to cited text no. 22
del Giudice P, Blanc V, de Rougemont A, Bes M, Lina G, Hubiche T, et al. Primary skin abscesses are mainly caused by Panton-Valentine leukocidin-positive Staphylococcus aureus strains. Dermatology 2009;219:299-302.  Back to cited text no. 23