The Effects of Hydroalcoholic Extract of Nigella sativa Seeds on Serum Estradiol and Prolactin Levels and obstetric Criteria due to Hypothyroidism in Rat

Document Type : Original Article

Authors
Roghayeh Pakdel 1
Hossien Hadjzadeh 2
Majid Mohammad Sadegh 2
Mahmoud Hosseini 3
Bahman Emami 1
Mousa-Al-Reza Hadjzadeh 3
1 Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2 Department of Clinical Science, Veterinary Medicine Faculty, Islamic Azad University, Garmsar Branch, Garmsar, Iran
3 Department of Physiology, School of Medicine, Mashhad University of Medical Sciences; Neurocognitive Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Abstract
Background: The aim of this study was investigation of the effects of Nigella sativa (NS) seeds on hypothyroid pregnant rats and their progenies. Materials and Methods: Hypothyroidism was induced by propylthiouracil (PTU) 0.03% in drinking water. Female rats were divided into seven groups: control, PTU, PTU-NS (100, 200, and 400 mg/kg), and NS (100 and 400 mg/kg). All treatments were done 20 days before mating and during pregnancy. The weight of rat dams and progenies, number of progenies and serum T4, estradiol and prolactin (PRL) levels in rat dams were measured for all groups. Results: Serum T4 in all PTU-NS groups before mating was significantly increased versus PTU group. Body weight of rat dams before mating in all groups of PTU-NS was increased versus PTU group by P < 0.001, P < 0.05, and P < 0.001, respectively and in NS 100 and NS 400 was increased versus control group (P < 0.001). The number of offspring was significantly decreased in PTU and PTU-NS versus control group. The weight of progenies in NS 400 was higher than control group (P < 0.001) and was increased in PTU-NS 200 and PTU-NS 400 versus PTU group by P < 0.001 and P < 0.05, respectively. Serum PRL level in rat dams in control, PTU, and PTU-NS groups were not statistically different between groups but significantly increased in NS 400 group when compared to control group. Estradiol levels were not significantly different in rat dams at 5 days after delivery. Conclusion: These results demonstrated that feeding of rat dams with NS extract before mating has positive protective effects on progenies. These effects may be due to antioxidant properties of NS in reducing oxidative stress and thyroid damages induced by PTU.

Keywords

1.
Hirsch D, Levy S, Nadler V, Kopel V, Shainberg B, Toledano Y. Pregnancy outcomes in women with severe hypothyroidism. Eur J Endocrinol 2013;169:313-20.  Back to cited text no. 1
[PUBMED]    
2.
Milanesi A, Brent GA. Management of hypothyroidism in pregnancy. Curr Opin Endocrinol Diabetes Obes 2011;18:304-9.  Back to cited text no. 2
[PUBMED]    
3.
Lahoti SK, Toppo L. Subclinical hypothyroidism and pregnancy outcomes. Annals of International Medical and Dental Research. 2012;1(3):324-6.  Back to cited text no. 3
    
4.
Casey BM, Dashe JS, Wells CE, McIntire DD, Byrd W, Leveno KJ, et al. Subclinical hypothyroidism and pregnancy outcomes. Obstet Gynecol 2005;105:239-45.  Back to cited text no. 4
[PUBMED]    
5.
Haddow JE; Thyroid Study Group. Subclinical hypothyroidism and pregnancy outcomes. Obstet Gynecol 2005;106:198.  Back to cited text no. 5
[PUBMED]    
6.
Verma I, Sood R, Juneja S, Kaur S. Prevalence of hypothyroidism in infertile women and evaluation of response of treatment for hypothyroidism on infertility. Int J Appl Basic Med Res 2012;2:17-9.  Back to cited text no. 6
[PUBMED]    
7.
Ouzounian S, Bringer-Deutsch S, Jablonski C, Théron-Gérard L, Snaifer E, Cédrin-Durnerin I, et al. Hypothyroidism: From the desire for pregnancy to delivery. Gynecol Obstet Fertil 2007;35:240-8.  Back to cited text no. 7
    
8.
Abalovich M, Mitelberg L, Allami C, Gutierrez S, Alcaraz G, Otero P, et al. Subclinical hypothyroidism and thyroid autoimmunity in women with infertility. Gynecol Endocrinol 2007;23:279-83.  Back to cited text no. 8
[PUBMED]    
9.
Krassas GE, Poppe K, Glinoer D. Thyroid function and human reproductive health. Endocr Rev 2010;31:702-55.  Back to cited text no. 9
[PUBMED]    
10.
Berbel P, Obregón MJ, Bernal J, Escobar del Rey F, Morreale de Escobar G. Iodine supplementation during pregnancy: A public health challenge. Trends Endocrinol Metab 2007;18:338-43.  Back to cited text no. 10
    
11.
Lakra K, Roy P. Pregnancy outcome of iodine deficiency: A study on tribal women in Orissa. Int J Res Dev Health 2013;1:61-8.  Back to cited text no. 11
    
12.
Ahmad A, Husain A, Mujeeb M, Khan SA, Najmi AK, Siddique NA, et al. Areview on therapeutic potential of Nigella sativa: A miracle herb. Asian Pac J Trop Biomed 2013;3:337-52.  Back to cited text no. 12
[PUBMED]    
13.
Gaafar H, Ragab AA, El-Reidy K. Effect of diet supplemented with pumpkin (Cucurbita moschata) and black seed (Nigella sativa) oils on performance of rabbits: 2-Productive and reproductive performance of does and their offspring. Rep Opin 2014;6:60-8.  Back to cited text no. 13
    
14.
Hadjzadeh MA, Mohammadian N, Rahmani Z, Rassouli FB. Effect of thymoquinone on ethylene glycol-induced kidney calculi in rats. Urol J 2008;5:149-55.  Back to cited text no. 14
[PUBMED]    
15.
Asgary S, Ghannadi A, Dashti G, Helalat A, Sahebkar A, Najafi S. Nigella sativa L. improves lipid profile and prevents atherosclerosis: Evidence from an experimental study on hypercholesterolemic rabbits. J Funct Foods 2013;5:228-34.  Back to cited text no. 15
    
16.
Hayatdavoudi P, Khajavi Rad A, Rajaei Z, Hadjzadeh MA. Renal injury, nephrolithiasis and Nigella sativa: A mini review. Avicenna J Phytomed 2016;6:1-8.  Back to cited text no. 16
[PUBMED]    
17.
Seghatoleslam M, Alipour F, Shafieian R, Hassanzadeh Z, Edalatmanesh MA, Sadeghnia HR, et al. The effects of Nigella sativa on neural damage after pentylenetetrazole induced seizures in rats. J Tradit Complement Med 2015;6:262-8.  Back to cited text no. 17
[PUBMED]    
18.
Houghton PJ, Zarka R, de las Heras B, Hoult JR. Fixed oil of Nigella sativa and derived thymoquinone inhibit eicosanoid generation in leukocytes and membrane lipid peroxidation. Planta Med 1995;61:33-6.  Back to cited text no. 18
[PUBMED]    
19.
Ali BH, Blunden G. Pharmacological and toxicological properties of Nigella sativa. Phytother Res 2003;17:299-305.  Back to cited text no. 19
[PUBMED]    
20.
Khalawi AA, Al-Robai AA, Khoja SM, Shaker A. Can Nigella sativa oil (NSO) reverse hypothyroid status induced by PTU in rat? Biochemical and histological studies. Life Sci J 2013; 10:802-811.  Back to cited text no. 20
    
21.
Jasim WK, Hassan MS, Keam GG. Study the effect of Nigella sativa on thyroid function and reproductive hormone of female rat. J Contemp Med Sci 2016;2:67-9.  Back to cited text no. 21
    
22.
Mousavi SH, Tayarani-Najaran Z, Asghari M, Sadeghnia HR. Protective effect of Nigella sativa extract and thymoquinone on serum/glucose deprivation-induced PC12 cells death. Cell Mol Neurobiol 2010;30:591-8.  Back to cited text no. 22
[PUBMED]    
23.
Farah N, Benghuzzi H, Tucci M, Cason Z. The effects of isolated antioxidants from black seed on the cellular metabolism of A549 cells. Biomed Sci Instrum 2005;41:211-6.  Back to cited text no. 23
[PUBMED]    
24.
Yaman I, Balikci E. Protective effects of Nigella sativa against gentamicin-induced nephrotoxicity in rats. Exp Toxicol Pathol 2010;62:183-90.  Back to cited text no. 24
[PUBMED]    
25.
Gray JP, Burgos DZ, Yuan T, Seeram N, Rebar R, Follmer R, et al. Thymoquinone, a bioactive component of Nigella sativa, normalizes insulin secretion from pancreatic ß-cells under glucose overload via regulation of malonyl-CoA. Am J Physiol Endocrinol Metab 2016;310:E394-404.  Back to cited text no. 25
[PUBMED]    
26.
Keshri G, Singh MM, Lakshmi V, Kamboj VP. Post-coital contraceptive efficacy of the seeds of Nigella sativa in rats. Indian J Physiol Pharmacol 1995;39:59-62.  Back to cited text no. 26
[PUBMED]    
27.
Juma FT, Hayfaa MA. The effects of Nigella sativa oil administration on some physiological and histological values of reproductive aspects of rats. Iraqi J Vet Med 2011;35:52-60.  Back to cited text no. 27
    
28.
Melmed S, Polonsky KS, Larsen PR, Kronenberg HM. Williams Textbook of Endocrinology. Canada. Elsevier Health Sciences; 2015.  Back to cited text no. 28
    
29.
Sharif SH, Elmahdi BM, Mohammed AM, Mohammed AH. The effects of Nigella sativa L. ethanolic extract on thyroid function in normal and alloxan-induced diabetic rats. Thyroid Res Pract 2012;9:48.  Back to cited text no. 29
  [Full text]  
Advanced Biomedical Research
Volume 2017, December
December 2017
Pages 1-6