Quercetin Prevents Body Weight Loss Due to the Using of Superparamagnetic Iron Oxide Nanoparticles in Rat

Document Type : Original Article

Authors

1 Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

2 Department of Biology, Cell, Molecular Biology and Biochemistry Division, Faculty of Sciences, University of Isfahan, Isfahan, Iran

3 Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran

4 Department of Clinical Science, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

Abstract

Background: Superparamagnetic iron oxide nanoparticles (SPION) have been largely considered for numerous applications in biomedicine such as magnetic resonance imaging, hyperthermia, cell tracking, anticancer treatment, and targeted delivery of drugs or genes. However, they may have side effects such body weight loss. Quercetin (QT), a strong antioxidant and free radical scavenger and a natural flavonoid, has a wide range of biological and therapeutic effects. In this study, the effect of QT on prevention of weight loss due to the using of SPION has been investigated. Materials and Methods: SPION and QT-SPION were administered orally at 50 and 100 mg/kg for 7 days. Then, the body weight was measured at the beginning and the end of the study. Results: Rats fed with 50 and 100 mg/kg SPION showed a significant weight loss, whereas those that fed with 50 mg/kg QT-SPION did not. A weight loss was observed in rats treated with 100 mg/kg of QT-SPION. Conclusions: The results of this study showed that quercetin could prevent weight loss due to the SPION.

Keywords

1.
Kumari A, Yadav SK, Pakade YB, Singh B, Yadav SC. Development of biodegradable nanoparticles for delivery of quercetin. Colloids Surf B Biointerfaces 2010;80:184-92.  Back to cited text no. 1
[PUBMED]    
2.
Wu TH, Yen FL, Lin LT, Tsai TR, Lin CC, Cham TM, et al. Preparation, physicochemical characterization, and antioxidant effects of quercetin nanoparticles. Int J Pharm 2008;346:160-8.  Back to cited text no. 2
    
3.
Bagad M, Khan ZA. Poly(n-butylcyanoacrylate) nanoparticles for oral delivery of quercetin: Preparation, characterization, and pharmacokinetics and biodistribution studies in Wistar rats. Int J Nanomedicine 2015;10:3921-35.  Back to cited text no. 3
    
4.
Manach C, Scalbert A, Morand C, Rémésy C, Jiménez L. Polyphenols: Food sources and bioavailability. Am J Clin Nutr 2004;79:727-47.  Back to cited text no. 4
    
5.
Chakraborty S, Stalin S, Das N, Choudhury ST, Ghosh S, Swarnakar S, et al. The use of nano-quercetin to arrest mitochondrial damage and MMP-9 upregulation during prevention of gastric inflammation induced by ethanol in rat. Biomaterials 2012;33:2991-3001.  Back to cited text no. 5
    
6.
Pilorget A, Berthet V, Luis J, Moghrabi A, Annabi B, Béliveau R, et al. Medulloblastoma cell invasion is inhibited by green tea (-) epigallocatechin-3-gallate. J Cell Biochem 2003;90:745-55.  Back to cited text no. 6
    
7.
Morimoto Y, Yasuhara T, Sugimoto A, Inoue A, Hide I, Akiyama M, et al. Anti-allergic substances contained in the pollen of Cryptomeria japonica possess diverse effects on the degranulation of RBL-2H3 cells. J Pharmacol Sci 2003;92:291-5.  Back to cited text no. 7
[PUBMED]    
8.
Ramos FA, Takaishi Y, Shirotori M, Kawaguchi Y, Tsuchiya K, Shibata H, et al. Antibacterial and antioxidant activities of quercetin oxidation products from yellow onion (Allium cepa) skin. J Agric Food Chem 2006;54:3551-7.  Back to cited text no. 8
[PUBMED]    
9.
Novakovic A, Gojkovic-Bukarica L, Peric M, Nezic D, Djukanovic B, Markovic-Lipkovski J, et al. The mechanism of endothelium-independent relaxation induced by the wine polyphenol resveratrol in human internal mammary artery. J Pharmacol Sci 2006;101:85-90.  Back to cited text no. 9
[PUBMED]    
10.
Lee H, Bae JH, Lee SR. Protective effect of green tea polyphenol EGCG against neuronal damage and brain edema after unilateral cerebral ischemia in gerbils. J Neurosci Res 2004;77:892-900.  Back to cited text no. 10
[PUBMED]    
11.
Duarte J, Pérez-Vizcaíno F, Zarzuelo A, Jiménez J, Tamargo J. Vasodilator effects of quercetin in isolated rat vascular smooth muscle. Eur J Pharmacol 1993;239:1-7.  Back to cited text no. 11
    
12.
Choi HJ, Kim JH, Lee CH, Ahn YJ, Song JH, Baek SH, et al. Antiviral activity of quercetin 7-rhamnoside against porcine epidemic diarrhea virus. Antiviral Res 2009;81:77-81.  Back to cited text no. 12
[PUBMED]    
13.
Kim HP, Son KH, Chang HW, Kang SS. Anti-inflammatory plant flavonoids and cellular action mechanisms. J Pharmacol Sci 2004;96:229-45.  Back to cited text no. 13
[PUBMED]    
14.
Stewart LK, Soileau JL, Ribnicky D, Wang ZQ, Raskin I, Poulev A, et al. Quercetin transiently increases energy expenditure but persistently decreases circulating markers of inflammation in C57BL/6J mice fed a high-fat diet. Metabolism 2008;57:S39-46.  Back to cited text no. 14
[PUBMED]    
15.
Davis JM, Murphy EA, Carmichael MD, Davis B. Quercetin increases brain and muscle mitochondrial biogenesis and exercise tolerance. Am J Physiol Regul Integr Comp Physiol 2009;296:R1071-7.  Back to cited text no. 15
[PUBMED]    
16.
Ossola B, Kääriäinen TM, Männistö PT. The multiple faces of quercetin in neuroprotection. Expert Opin Drug Saf 2009;8:397-409.  Back to cited text no. 16
    
17.
Dajas F. Life or death: Neuroprotective and anticancer effects of quercetin. J Ethnopharmacol 2012;143:383-96.  Back to cited text no. 17
[PUBMED]    
18.
Dajas F, Andrés AC, Florencia A, Carolina E, Felicia RM. Neuroprotective actions of flavones and flavonols: Mechanisms and relationship to flavonoid structural features. Cent Nerv Syst Agents Med Chem 2013;13:30-5.  Back to cited text no. 18
    
19.
Nichols M, Zhang J, Polster BM, Elustondo PA, Thirumaran A, Pavlov EV, et al. Synergistic neuroprotection by epicatechin and quercetin: Activation of convergent mitochondrial signaling pathways. Neuroscience 2015;308:75-94.  Back to cited text no. 19
    
20.
Lee YJ, Bernstock JD, Nagaraja N, Ko B, Hallenbeck JM. Global SUMOylation facilitates the multimodal neuroprotection afforded by quercetin against the deleterious effects of oxygen/glucose deprivation and the restoration of oxygen/glucose. J Neurochem 2016;138:101-16.  Back to cited text no. 20
[PUBMED]    
21.
Davis JM, Murphy EA, Carmichael MD. Effects of the dietary flavonoid quercetin upon performance and health. Curr Sports Med Rep 2009;8:206-13.  Back to cited text no. 21
[PUBMED]    
22.
Kressler J, Millard-Stafford M, Warren GL. Quercetin and endurance exercise capacity: A systematic review and meta-analysis. Med Sci Sports Exerc 2011;43:2396-404.  Back to cited text no. 22
[PUBMED]    
23.
Aguirre L, Arias N, Macarulla MT, Gracia A, Portillo MP. Beneficial effects of quercetin on obesity and diabetes. Open Nutraceuticals J 2011;4:189-98.  Back to cited text no. 23
    
24.
Jung CH, Cho I, Ahn J, Jeon TI, Ha TY. Quercetin reduces high-fat diet-induced fat accumulation in the liver by regulating lipid metabolism genes. Phytother Res 2013;27:139-43.  Back to cited text no. 24
[PUBMED]    
25.
Rivera L, Morón R, Sánchez M, Zarzuelo A, Galisteo M. Quercetin ameliorates metabolic syndrome and improves the inflammatory status in obese Zucker rats. Obesity (Silver Spring) 2008;16:2081-7.  Back to cited text no. 25
    
26.
Qureshi AA, Tan X, Reis JC, Badr MZ, Papasian CJ, Morrison DC, et al. Inhibition of nitric oxide in LPS-stimulated macrophages of young and senescent mice by δ-tocotrienol and quercetin. Lipids Health Dis 2011;10:239.  Back to cited text no. 26
[PUBMED]    
27.
Azuma K, Ippoushi K, Terao J. Evaluation of tolerable levels of dietary quercetin for exerting its antioxidative effect in high cholesterol-fed rats. Food Chem Toxicol 2010;48:1117-22.  Back to cited text no. 27
[PUBMED]    
28.
Casuso RA, Martínez-López EJ, Hita-Contreras F, Camiletti-Moirón D, Martínez-Amat A. Quercetin effects on weight gain and caloric intake in exercised rats. Biol Sport 2014;31:63-7.  Back to cited text no. 28
    
29.
Rivera F, Urbanavicius J, Gervaz E, Morquio A, Dajas F. Some aspects of the in vivo neuroprotective capacity of flavonoids: Bioavailability and structure-activity relationship. Neurotox Res 2004;6:543-53.  Back to cited text no. 29
[PUBMED]    
30.
Blasina F, Vaamonde L, Silvera F, Tedesco AC, Dajas F. Intravenous nanosomes of quercetin improve brain function and hemodynamic instability after severe hypoxia in newborn piglets. Neurochem Int 2015;89:149-56.  Back to cited text no. 30
[PUBMED]    
31.
Galho AR, Cordeiro MF, Ribeiro SA, Marques MS, Antunes MF, Luz DC, et al. Protective role of free and quercetin-loaded nanoemulsion against damage induced by intracerebral haemorrhage in rats. Nanotechnology 2016;27:175101.  Back to cited text no. 31
[PUBMED]    
32.
Barreto A, Santiago V, Mazzetto S, Denardin JC, Lavín R, Mele G, et al. Magnetic nanoparticles for a new drug delivery system to control quercetin releasing for cancer chemotherapy. J Nanopart Res 2011;13:6545-53.  Back to cited text no. 32
    
33.
Kumar SR, Priyatharshni S, Babu VN, Mangalaraj D, Viswanathan C, Kannan S, et al. Quercetin conjugated superparamagnetic magnetite nanoparticles for in-vitro analysis of breast cancer cell lines for chemotherapy applications. J Colloid Interface Sci 2014;436:234-42.  Back to cited text no. 33
[PUBMED]    
34.
Boyer C, Whittaker MR, Bulmus V, Liu J, Davis TP. The design and utility of polymer-stabilized iron-oxide nanoparticles for nanomedicine applications. NPG Asia Mater. 2010;2:23-30.  Back to cited text no. 34
    
35.
Szalay B, Tátrai E, Nyírő G, Vezér T, Dura G. Potential toxic effects of iron oxide nanoparticles in in vivo and in vitro experiments. J Appl Toxicol 2012;32:446-53.  Back to cited text no. 35
    
36.
Zhu MT, Wang Y, Feng WY, Wang B, Wang M, Ouyang H, et al. Oxidative stress and apoptosis induced by iron oxide nanoparticles in cultured human umbilical endothelial cells. J Nanosci Nanotechnol 2010;10:8584-90.  Back to cited text no. 36
[PUBMED]    
37.
Khan MI, Mohammad A, Patil G, Naqvi SA, Chauhan LK, Ahmad I, et al. Induction of ROS, mitochondrial damage and autophagy in lung epithelial cancer cells by iron oxide nanoparticles. Biomaterials 2012;33:1477-88.  Back to cited text no. 37
    
38.
Alarifi S, Ali D, Alkahtani S, Alhader MS. Iron oxide nanoparticles induce oxidative stress, DNA damage, and caspase activation in the human breast cancer cell line. Biol Trace Elem Res 2014;159:416-24.  Back to cited text no. 38
[PUBMED]    
39.
Pool H, Quintanar D, de Dios Figueroa J, Mano CM, Bechara JE, Godínez LA, et al. Antioxidant effects of quercetin and catechin encapsulated into PLGA nanoparticles. J nanomater 2012;2012:86.  Back to cited text no. 39
    
40.
Milton Prabu S, Shagirtha K, Renugadevi J. Quercetin in combination with vitamins (C and E) improves oxidative stress and renal injury in cadmium intoxicated rats. Eur Rev Med Pharmacol Sci 2010;14:903-14.  Back to cited text no. 40
[PUBMED]    
41.
Lin HC, Cheng TH, Chen YC, Juan SH. Mechanism of heme oxygenase-1 gene induction by quercetin in rat aortic smooth muscle cells. Pharmacology 2004;71:107-12.  Back to cited text no. 41
[PUBMED]