Extract from Teucrium polium L. Protects Rat Heart against Oxidative Stress Induced by Ischemic–reperfusion Injury

Document Type : Original Article


1 Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences; Neurogenic Inflammation Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran

2 Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran

3 Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran


Background: The deleterious effect of oxidative stress on myocardial ischemia–reperfusion (I/R) has already been shown in previous studies. Since Teucrium polium has anti-oxidative and cardio-protective properties, the aim of this study was to investigate the effects of this plant on I/R injuries in the isolated rat heart. Materials and Methods: The myocardial I/R injury of rat was created by Langendorff retrograde perfusion technology. The heart was preperfused with Krebs–Henseleit (K-H) solution containing T. polium extract for 20 min before 20 min global ischemia, and then the reperfusion with K-H bicarbonate buffer was conducted for 40 min. The left ventricular developed pressure and the maximum up/down rate of the left ventricular pressure (±dp/dtmax) were recorded by physiological recorder as the myocardial function. Lactate dehydrogenase (LDH) and creatine kinase (CK) activities in the effluent were measured to determine the myocardial injury degree. Thiobarbituric acid reactive substances (TBARS), total thiol groups (−SH), superoxide anion dismutase (SOD), and catalase (CAT) in myocardial tissue were detected to determine the oxidative stress degree. Results: The results showed that the pretreatment with T. polium significantly enhanced cardiac parameters and the coronary artery flow, decreased the LDH, CK activities, and TBARS level, whereas it increased − SH groups, SOD and CAT activities. Conclusions: Our findings indicated that T. polium could provide protection for heart against the I/R injury which may be related to the improvement of myocardial oxidative stress states.


Zeng XJ, Zhang LK, Wang HX, Lu LQ, Ma LQ, Tang CS. Apelin protects heart against ischemia/reperfusion injury in rat. Peptides 2009;30:1144-52.  Back to cited text no. 1
Wang D, Guo X, Zhou M, Han J, Han B, Sun X. Cardioprotective effect of the aqueous extract of lavender flower against myocardial ischemia/reperfusion injury. Journal of Chemistry 2014.  Back to cited text no. 2
Chen EP, Bittner HB, Davis RD, Folz RJ, Van Trigt P. Extracellular superoxide dismutase transgene overexpression preserves postischemic myocardial function in isolated murine hearts. Circulation 1996;94 9 Suppl:II412-7.  Back to cited text no. 3
Du Y, Guo H, Lou H. Grape seed polyphenols protect cardiac cells from apoptosis via induction of endogenous antioxidant enzymes. J Agric Food Chem 2007;55:1695-701.  Back to cited text no. 4
Ambrosio G, Zweier JL, Duilio C, Kuppusamy P, Santoro G, Elia PP, et al. Evidence that mitochondrial respiration is a source of potentially toxic oxygen free radicals in intact rabbit hearts subjected to ischemia and reflow. J Biol Chem 1993;268:18532-41.  Back to cited text no. 5
Maulik N, Sharma HS, Das DK. Induction of the haem oxygenase gene expression during the reperfusion of ischemic rat myocardium. J Mol Cell Cardiol 1996;28:1261-70.  Back to cited text no. 6
Swaminathan JK, Khan M, Mohan IK, Selvendiran K, Niranjali Devaraj S, Rivera BK, et al. Cardioprotective properties of Crataegus oxycantha extract against ischemia-reperfusion injury. Phytomedicine 2010;17:744-52.  Back to cited text no. 7
Devi R, Banerjee SK, Sood S, Dinda AK, Maulik SK. Extract from Clerodendron colebrookianum Walp protects rat heart against oxidative stress induced by ischemic-reperfusion injury (IRI). Life Sci 2005;77:2999-3009.  Back to cited text no. 8
Augusti KT, Arathy SL, Asha R, Ramakrishanan J, Zaira J, Lekha V, et al. Acomparative study on the beneficial effects of garlic (Allium sativum Linn), amla (Emblica Officinalis Gaertn) and onion (Allium cepa Linn) on the hyperlipidemia induced by butter fat and beef fat in rats. Indian J Exp Biol 2001;39:760-6.  Back to cited text no. 9
Helen A, Krishnakumar K, Vijayammal PL, Augusti KT. Antioxidant effect of onion oil (Allium cepa. Linn) on the damages induced by nicotine in rats as compared to alpha-tocopherol. Toxicol Lett 2000;116:61-8.  Back to cited text no. 10
Bahramikia S, Yazdanparast R. Phytochemistry and medicinal properties of Teucrium polium L. (Lamiaceae). Phytother Res 2012;26:1581-93.  Back to cited text no. 11
Piozzi F, Bruno M, Rosselli S, Maggio A. Advances on the chemistry of furano-diterpenoids from Teucrium genus. Heterocycles 2005;65:1221-34.  Back to cited text no. 12
Said O, Khalil K, Fulder S, Azaizeh H. Ethnopharmacological survey of medicinal herbs in Israel, the Golan Heights and the West Bank region. J Ethnopharmacol 2002;83:251-65.  Back to cited text no. 13
Zargari A. Vol. 4. Tehran: Tehran University Press; 1997. Medicinal Plants; p. 103.  Back to cited text no. 14
Esmaeili MA, Yazdanparast R. Hypoglycaemic effect of Teucrium polium: Studies with rat pancreatic islets. J Ethnopharmacol 2004;95:27-30.  Back to cited text no. 15
Ljubuncic P, Dakwar S, Portnaya I, Cogan U, Azaizeh H, Bomzon A. Aqueous extracts of Teucrium polium possess remarkable antioxidant activity in vitro. Evid Based Complement Alternat Med 2006;3:329-38.  Back to cited text no. 16
Abdollahi M, Karimpour H, Monsef-Esfehani HR. Antinociceptive effects of Teucrium polium L total extract and essential oil in mouse writhing test. Pharmacol Res 2003;48:31-5.  Back to cited text no. 17
Couladis M, Tzakou O, Verykokidou E, Harvala C. Screening of some Greek aromatic plants for antioxidant activity. Phytother Res 2003;17:194-5.  Back to cited text no. 18
Niazmand S, Erfanian Ahmadpoor M, Moosavian M, Derakhshan M. The positive inotropic and chronotropic effects of Teucrium polium L. extract on guinea pig isolated heart. Pharmacologyonline 2008;2:588-94.  Back to cited text no. 19
Bello R, Calatayud S, Moreno L, Beltran B, Primo Yufera E, Esplugues J. Effects on arterial blood pressure of the methanol extracts from different Teucrium species. Phytother Res 1997;11:330-1.  Back to cited text no. 20
Mahmoudabady M, Shafei MN, Niazmand S, Khodaee E. The effects of hydroalchoholic extract of Teucrium polium L. on hypertension induced by angiotensin II in rats. Int J Prev Med 2014;5:1255-60.  Back to cited text no. 21
Rasekh HR, Khoshnood-Mansourkhani MJ, Kamalinejad M. Hypolipidemic effects of Teucrium polium in rats. Fitoterapia 2001;72:937-9.  Back to cited text no. 22
Niazmand S, Esparham M, Hassannia T, Derakhshan M. Cardiovascular effects of Teucrium polium L. extract in rabbit. Pharmacogn Mag 2011;7:260-4.  Back to cited text no. 23
Yu J, Wang L, Akinyi M, Li Y, Duan Z, Zhu Y, et al. Danshensu protects isolated heart against ischemia reperfusion injury through activation of Akt/ERK1/2/Nrf2 signaling. Int J Clin Exp Med 2015;8:14793-804.  Back to cited text no. 24
Niazmand S, Fereidouni E, Mahmoudabady M, Mousavi SM. Endothelium-independent vasorelaxant effects of hydroalcoholic extract from Nigella sativa seed in rat aorta: The roles of Ca2+ and K+ channels. Biomed Res Int 2014;2014:247054.  Back to cited text no. 25
Janero DR. Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radic Biol Med 1990;9:515-40.  Back to cited text no. 26
Sharma JB, Sharma A, Bahadur A, Vimala N, Satyam A, Mittal S. Oxidative stress markers and antioxidant levels in normal pregnancy and pre-eclampsia. Int J Gynaecol Obstet 2006;94:23-7.  Back to cited text no. 27
Madesh M, Balasubramanian KA. Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide. Indian J Biochem Biophys 1998;35:184-8.  Back to cited text no. 28
Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121-6.  Back to cited text no. 29
Fan H, Yang L, Fu F, Xu H, Meng Q, Zhu H, et al. Cardioprotective effects of salvianolic acid a on myocardial ischemia-reperfusion injury in vivo and in vitro. Evid Based Complement Alternat Med 2012;2012:508938.  Back to cited text no. 30
Dhalla NS, Elmoselhi AB, Hata T, Makino N. Status of myocardial antioxidants in ischemia-reperfusion injury. Cardiovasc Res 2000;47:446-56.  Back to cited text no. 31
Akhlaghi M, Bandy B. Mechanisms of flavonoid protection against myocardial ischemia-reperfusion injury. J Mol Cell Cardiol 2009;46:309-17.  Back to cited text no. 32
Eskandary H, Rajabalian S, Yazdi T, Eskandari M, Fatehi K, Ganjooei NA. Evaluation of cytotoxic effect of Teucrium polium on a new glioblastoma multiforme cell line (REYF-1) using MTT and soft agar clonogenic assays. Int J Pharmacol 2007;3:435-7.  Back to cited text no. 33
Nematollahi-Mahani SN, Rezazadeh-Kermani M, Mehrabani M, Nakhaee N. Cytotoxic effects of Teucrium polium on some established cell lines. Pharm Biol 2007;45:295-8.  Back to cited text no. 34
Mazokopakis E, Lazaridou S, Tzardi M, Mixaki J, Diamantis I, Ganotakis E. Acute cholestatic hepatitis caused by Teucrium polium L. Phytomedicine 2004;11:83-4.  Back to cited text no. 35
Savvidou S, Goulis J, Giavazis I, Patsiaoura K, Hytiroglou P, Arvanitakis C. Herb-induced hepatitis by Teucrium polium L.: Report of two cases and review of the literature. Eur J Gastroenterol Hepatol 2007;19:507-11.  Back to cited text no. 36