Establishment and Development of the First Biobank of Inflammatory Bowel Disease, Suspected to Primary Immunodeficiency Diseases in Iran

Document Type : Original Article

Authors
Roya Sherkat 1
Soodabeh Rostami 2
Majid Yaran 3
Mohammad Hassan Emami 4
Hosein Saneian 5
Hamid Tavakoli 6
Peyman Adibi 6
Mahdieh Behnam 7
Saba Sheykhbahaei 1
Bahram Bagherpour 1
Razieh Khoshnevisan 1
Somayeh Najafi 1
1 Acquired Immunodeficiency Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
2 Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
3 Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
4 Poursina Hakim Research Institute, Isfahan, Iran
5 Department of Pediatrics, Isfahan University of Medical Sciences, Isfahan, Iran
6 Integrative Functional Gastroenterology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
7 Medical Genetics Laboratory, Alzahra University Hospital, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Background: Inflammatory bowel disease (IBD) might be an immunodeficiency rather than an excessive inflammatory reaction. IBD, suspected to primary immunodeficiency diseases biobank (IBDSPIDB) as a resource for researches can help improve the prevention, diagnosis, and illness treatment and the health promotion throughout the society. Therefore, we launched the biobank of IBDSPID for the first time in Iran. Materials and Methods: This study was designed to provide the IBDSPIDB to have a high-quality DNA, RNA, and cDNA. Among of 365 patients, 39 have inclusion criteria that were as below: (1) IBD diagnosis before 5 years of age. (2) Resistance to conventional therapy of IBD. (3) Severe IBD. (4) Signs of SPID (including ear infections or pneumonia or recurrent sinus within the 1-year period; failure to thrive; poor response to the prolonged use of antibiotics; persistent thrush or skin abscesses; or a family history of PID). Results: Out of 39 patients, 51.3% were males. The mean age was 32.92 ± 15.90 years old. Ulcerative colitis (79.5%) was more than Crohn's disease. The majority of patients (50.0%) had severe IBDSPID. Resistance to drugs and consanguinity was 12.9% and 47.4%, respectively. Age at onset in 65.8% of patients was after 17 years old. Patients with autoimmune, allergy, and immunodeficiency disease history were 33.3%, 33.3%, and 10.36%, respectively. RNA and cDNA yields large quantities of high-quality DNA obtained and stored. Conclusion: Our biobank would be valuable for future genetic and molecular study to be more about the relation between IBD and PID
Keywords
Biobank
inflammatory bowel diseases
primary immunodeficiency diseases
1.
Abraham C, Cho JH. Inflammatory bowel disease. N Engl J Med 2009;361:2066-78.  Back to cited text no. 1
[PUBMED]    
2.
Podolsky DK. Inflammatory bowel disease. N Engl J Med 2002;347:417-29.  Back to cited text no. 2
    
3.
Fiocchi C. Inflammatory bowel disease pathogenesis: Where are we? J Gastroenterol Hepatol 2015;30 Suppl 1:12-8.  Back to cited text no. 3
    
4.
Malys MK, Campbell L, Malys N. Symbiotic and antibiotic interactions between gut commensal microbiota and host immune system. Medicina (Kaunas) 2015;51:69-75.  Back to cited text no. 4
    
5.
Glocker E, Grimbacher B. Inflammatory bowel disease: Is it a primary immunodeficiency? Cell Mol Life Sci 2012;69:41-8.  Back to cited text no. 5
    
6.
Fujimoto T, Kato J, Nasu J, Kuriyama M, Okada H, Yamamoto H, et al. Change of clinical characteristics of ulcerative colitis in Japan: Analysis of 844 hospital-based patients from 1981 to 2000. Eur J Gastroenterol Hepatol 2007;19:229-35.  Back to cited text no. 6
    
7.
Cosnes J, Gower-Rousseau C, Seksik P, Cortot A. Epidemiology and natural history of inflammatory bowel diseases. Gastroenterology 2011;140:1785-94.  Back to cited text no. 7
    
8.
Aghazadeh R, Zali MR, Bahari A, Amin K, Ghahghaie F, Firouzi F. Inflammatory bowel disease in Iran: A review of 457 cases. J Gastroenterol Hepatol 2005;20:1691-5.  Back to cited text no. 8
    
9.
Vahedi H, Merat S, Momtahen S, Olfati G, Kazzazi AS, Tabrizian T, et al. Epidemiologic characteristics of 500 patients with inflammatory bowel disease in Iran studied from 2004 through 2007. Arch Iran Med 2009;12:454-60.  Back to cited text no. 9
    
10.
Trauth JM, Musa D, Siminoff L, Jewell IK, Ricci E. Public attitudes regarding willingness to participate in medical research studies. J Health Soc Policy 2000;12:23-43.  Back to cited text no. 10
    
11.
McCusker C, Warrington R. Primary immunodeficiency. Allergy Asthma Clin Immunol 2011;7 Suppl 1:S11.  Back to cited text no. 11
    
12.
de Montgolfier S, Moutel G, Duchange N, Theodorou I, Hervé C, Leport C; APROCO Study Group. Ethical reflections on pharmacogenetics and DNA banking in a cohort of HIV-infected patients. Pharmacogenetics 2002;12:667-75.  Back to cited text no. 12
    
13.
Rohr J, Pannicke U, Döring M, Schmitt-Graeff A, Wiech E, Busch A, et al. Chronic inflammatory bowel disease as key manifestation of atypical ARTEMIS deficiency. J Clin Immunol 2010;30:314-20.  Back to cited text no. 13
    
14.
Salzer E, Kansu A, Sic H, Májek P, Ikinciogullari A, Dogu FE, et al. Early-onset inflammatory bowel disease and common variable immunodeficiency-like disease caused by IL-21 deficiency. J Allergy Clin Immunol 2014;133:1651-9.e12.  Back to cited text no. 14
    
15.
Denson LA, Long MD, McGovern DP, Kugathasan S, Wu GD, Young VB, et al. Challenges in IBD research: Update on progress and prioritization of the CCFA's research agenda. Inflamm Bowel Dis 2013;19:677-82.  Back to cited text no. 15
    
16.
Mota-Vieira L, Pacheco PR, Almeida ML, Cabral R, Carvalho J, Branco CC, et al. Human DNA bank in Sao Miguel Island (Azores): A resource for genetic diversity studies. Int Congr Ser 2006;1288:388-90.  Back to cited text no. 16
    
17.
García-Merino I, de Las Cuevas N, Jiménez JL, Gallego J, Gómez C, Prieto C, et al. The Spanish HIV BioBank: A model of cooperative HIV research. Retrovirology 2009;6:27.  Back to cited text no. 17
    
18.
Isaian A, Moin M, Pourpak Z, Rezaei N, Aghamohammadi A, Movahedi M, et al. DNA banking of primary immunodeficiency disorders in Iran. Iran J Allergy Asthma Immunol 2006;5:201-2.  Back to cited text no. 18
Advanced Biomedical Research
Volume 2018, March
March 2018
Pages 1-4