Background: At workplaces, the workers exposed to several harmful agents such as physical, chemical, and biological pollutant that cause occupational diseases. There are a lot of jobs that not allow a certain pattern of exposure to this agent. In this article, we introduce a technology named radio-frequency identification (RFID) to estimate the exposure time of workers to harmful agent. Materials and Methods: This applied study was carried out experimentally in an industry in Isfahan province, Iran. Twenty-nine participants selected from the workers without a fixed pattern of exposure. Two methods used to measure the exposure time of them. The first method was based on a self-made RFID device and conducted by the workers. The second method performed by stopwatch, which was carried out by the occupational hygienist. The results were analyzed using SPSS 20 and descriptive statistics, Spearman correlation coefficient, and paired samples t-test. Results: The mean age of the participants was 36.48 ± 5.889 and job experience was 9.06 ± 6.316 years. Spearman correlation coefficient shows that there is a significant correlation between the exposure times measured by the workers and occupational hygienist (R in all zones was higher than 0.9, P < 0.05). Paired samples t-test shows that there are no significant differences between the mean exposure times measured by the workers and occupational hygienist in each zone and in all the zones (P > 0.05). Conclusion: RFID technology is an appropriate method to evaluate the exposure time of workers without fixed pattern of exposure to causative factors of occupational diseases. It can also be used in other fields of occupational health engineering.