Renal Tissue Damages and Its Antioxidant Status Improved by Crab Shell Extract in Streptozotocin-induced Diabetic Rat

Document Type : Original Article


1 Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran

2 Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran


Background: Diabetic nephropathy is a complex and multifactorial adverse effect of diabetes mellitus (DM). Crab shell as a natural product is supposed to have antioxidant effect which is one of the important mechanisms to improve DM. The aim of this study was to investigate the effect of crab shell extract (CSE) on the histopathology and antioxidant status of kidney in diabetic rats. Materials and Methods: Forty-two adult Wistar rats (210 ± 10 g) were divided into six groups (n = 7). Streptozotocin (50 mg/kg) was administered interaperitonealy (IP) for inducing diabetes. Rats were treated for 14 days by CSE with 100, 200, and 400 mg/kg doses IP. Fasting blood glucose, body, and renal weight were evaluated. The antioxidant status of kidney's tissue was evaluated by determining the level of ferric-reducing antioxidant power (FRAP). Furthermore, urine samples were used to determine nitric oxide (NO) levels. Microscopic slides were prepared to compare kidney histology between groups. Data were analyzed by one-way analysis of variance with post hoc Tukey's test, and P < 0.05 was considered statistically significant. Results: CSE induced a significant reduction in blood glucose (P = 0.01) and a significant increase in total antioxidant capacity (FRAP) (P = 0.004). Furthermore, urine NO was decreased significantly (P = 0.000). The extract improved renal tissue changes caused by diabetes. Conclusion: CSE improved antioxidant status and diabetic histological changes of rat kidney, and it could be an alternative complementary therapy in diabetic-associated disorders.


Petersmann A, Nauck M, Müller-Wieland D, Kerner W, Müller UA, Landgraf R, et al. Definition, classification and diagnosis of diabetes mellitus. Exp Clin Endocrinol Diabetes 2018;126:406-410.  Back to cited text no. 1
Ozougwu J, Obimba K, Belonwu C, Unakalamba C. The pathogenesis and pathophysiology of type 1 and type 2 diabetes mellitus. J Physiol Pathophysiol 2013;4:46-57.  Back to cited text no. 2
Li W, Wang G, Lu X, Jiang Y, Xu L, Zhao X. Lycopene ameliorates renal function in rats with streptozotocin-induced diabetes. Int J Clin Exp Pathol 2014;7:5008-5015.  Back to cited text no. 3
Chen KH, Hung CC, Hsu HH, Jing YH, Yang CW, Chen JK. Resveratrol ameliorates early diabetic nephropathy associated with suppression of augmented TGF-β/smad and ERK1/2 signaling in streptozotocin-induced diabetic rats. Chem Biol Interact 2011;190:45-53.  Back to cited text no. 4
Ghanbari E, Nejati V, Khazaei M. Improvement in serum biochemical alterations and oxidative stress of liver and pancreas following use of royal jelly in streptozotocin-induced diabetic rats. Cell J 2016;18:362-370.  Back to cited text no. 5
Nowotny K, Jung T, Höhn A, Weber D, Grune T. Advanced glycation end products and oxidative stress in type 2 diabetes mellitus. Biomolecules 2015;5:194-222.  Back to cited text no. 6
Ghanbari E, Ansarian A, Yosefzaei F, Khazaei M. Study of antioxidant status and oxidative stress in diabetic rats treated with royal jelly. J Zanjan Univ Med Sci 2017;25:82-93.  Back to cited text no. 7
Ghayur MN, Krepinsky JC, Janssen LJ. Contractility of the renal glomerulus and mesangial cells: Lingering doubts and strategies for the future. Med Hypotheses Res 2008;4:1-9.  Back to cited text no. 8
Kalra S, Mukherjee JJ, Venkataraman S, Bantwal G, Shaikh S, Saboo B, et al. Hypoglycemia: The neglected complication. Indian J Endocrinol Metab 2013;17:819-834.  Back to cited text no. 9
Al-Quraishy S, Dkhil MA, Abdel Moneim AE. Anti-hyperglycemic activity of selenium nanoparticles in streptozotocin-induced diabetic rats. Int J Nanomedicine 2015;10:6741-6756.  Back to cited text no. 10
Khazaei M, Bayat P, Ghanbari A, Khazaei S, Feizian M, Khodaei A, et al. Protective effects of subchronic caffeine administration on cisplatin induced urogenital toxicity in male mice. Indian J Exp Biol 2012;50:638-644.  Back to cited text no. 11
Kwoczek M, Szefer P, Hać E, Grembecka M. Essential and toxic elements in seafood available in Poland from different geographical regions. J Agric Food Chem 2006;54:3015-3024.  Back to cited text no. 12
Ngo DH, Vo TS, Ngo DN, Kang KH, Je JY, Pham HN, et al. Biological effects of chitosan and its derivatives. Food Hydrocol 2015;51:200-216.  Back to cited text no. 13
Motiei M, Kashanian S, Lucia LA, Khazaei M. Intrinsic parameters for the synthesis and tuned properties of amphiphilic chitosan drug delivery nanocarriers. J Control Release 2017;260:213-225.  Back to cited text no. 14
Makalani F, Khazaei MR, Ghanbari E, Khazaei M. Crab shell extract improves serum biochemical markers and histological changes of pancreas in diabetic rats. Int J Morphol 2017;35:1437-1443.  Back to cited text no. 15
Liu B, Liu WS, Han BQ, Sun YY. Antidiabetic effects of chitooligosaccharides on pancreatic islet cells in streptozotocin-induced diabetic rats. World J Gastroenterol 2007;13:725-7231.  Back to cited text no. 16
Yoon HJ, Moon ME, Park HS, Kim HW, Im SY, Lee JH, et al. Effects of chitosan oligosaccharide (COS) on the glycerol-induced acute renal failurein vitro and in vivo. Food Chem Toxicol 2008;46:710-716.  Back to cited text no. 17
Furman BL. Streptozotocin-induced diabetic models in mice and rats. Curr Protoc Pharmacol 2015;70:5.47.1-20.  Back to cited text no. 18
Raza H, John A. Streptozotocin-induced cytotoxicity, oxidative stress and mitochondrial dysfunction in human hepatoma HepG2 cells. Int J Mol Sci 2012;13:5751-5767.  Back to cited text no. 19
Rezakhani L, Rashidi Z, Mirzapur P, Khazaei M. Antiproliferatory effects of crab shell extract on breast cancer cell line (MCF7). J Breast Cancer 2014;17:219-225.  Back to cited text no. 20
Khazaei M, Roshankhah S, Ghorbani R, Chobsaz F. Sildenafil effect on nitric oxide secretion by normal human endometrial epithelial cells cultured in vitro. Int J Fertil Steril 2011;5:142-147.  Back to cited text no. 21
Chavez R, Fraser DJ, Bowen T, Jenkins RH, Nesargikar P, Pino-Chavez G, et al. Kidney ischaemia reperfusion injury in the rat: The EGTI scoring system as a valid and reliable tool for histological assessment. J Histol Histopathol 2016;3:1-7.  Back to cited text no. 22
Zafar M, Naqvi SN, Ahmed M, Kaimkhani ZA. Altered kidney morphology and enzymes in streptozotocin induced diabetic rats. Int J Morphol 2009;27: 783-790.  Back to cited text no. 23
Cakatay U, Kayali R. The evaluation of altered redox status in plasma and mitochondria of acute and chronic diabetic rats. Clin Biochem 2006;39:907-912.  Back to cited text no. 24
Rani AJ, Mythili SV. Study on total antioxidant status in relation to oxidative stress in type 2 diabetes mellitus. J Clin Diagn Res 2014;8:108-110.  Back to cited text no. 25
Khazaei MR, Makalani F, Ghanbari E, Fayzemahdavi M, Khazaei M. An overview of effective herbal and antioxidant compounds on diabetes. J Contemp Med Sci 2018;4:126-133.  Back to cited text no. 26
Zhang J, Wang Y, Li L, Zhang R, Guo R, Li H, et al. Diabetic retinopathy may predict the renal outcomes of patients with diabetic nephropathy. Ren Fail 2018;40:243-251.  Back to cited text no. 27
Chawla A, Chawla R, Jaggi S. Microvasular and macrovascular complications in diabetes mellitus: Distinct or continuum? Indian J Endocrinol Metab 2016;20:546-551.  Back to cited text no. 28
Azuma K, Osaki T, Minami S, Okamoto Y. Anticancer and anti-inflammatory properties of chitin and chitosan oligosaccharides. J Funct Biomater 2015;6:33-49.  Back to cited text no. 29
Choi EH, Yang HP, Chun HS. Chitooligosaccharide ameliorates diet-induced obesity in mice and affects adipose gene expression involved in adipogenesis and inflammation. Nutr Res 2012;32:218-228.  Back to cited text no. 30
Suleria HA, Osborne S, Masci P, Gobe G. Marine-based nutraceuticals: An innovative trend in the food and supplement industries. Mar Drugs 2015;13:6336-6351.  Back to cited text no. 31