Effect of a selection of skin penetration enhancers on topical anti-inflammatory effect of Boswellic acids in carrageenan-induced paw edema in rats

Document Type : Original Article

Authors

1 Department of Pharmacology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

2 Department of Pharmacology, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Boswellia species have been used for treatment of chronic inflammatory disease. Several studies have documented the anti-inflammatory effect of Boswellic acids (BAs) after systemic administration. This study was aimed to evaluate the effect of some skin penetration enhancers on topical anti-inflammatory effect of BAs in rats. Materials and Methods: Male Wistar rats weighting 180–220 were used. Anti-inflammatory activity was assessed using carrageenan test. BAs dissolved in ethanol, propylene glycol 2%, 5%, olive oil and applied topically. Menthol, D-limonene, or eucalyptus oil 0.5%, 1% were also tested as other skin penetration enhancers and applied topically 30 min prior to subplantar injection of carrageenan into the right hind paw of rats. The volume of the paw was measured at 0 and 4 h after carrageenan with a digital plethysmometer and the difference was used as an index of inflammation. Piroxicam gel was used as a standard drug. Results: A 4% ethanolic solution of BAs showed significant anti-inflammatory effect. Propylene glycol (2% and 5%) in alcohol did not change the effect. Olive oil also enhanced penetration of BAs. Menthol 0.5%, 1% and D-limonene 0.5%, 1% did not show any significant change compared to olive oil alone. In the present study, eucalyptus oil 1% in olive oil was known as the best carrier for transdermal delivery of BAs. Conclusion: BAs have considerable topical anti-inflammatory effects and olive oil alone or especially in combination with eucalyptus oil can be promising vehicles for skin penetration of topical BAs.

Keywords

1.
Dhingra AK, Chopra B, Dua JS, Parsad DN. New insight on inflammation and its management: A review. J Innov Pharm Biol Sci 2017;4:117-26.  Back to cited text no. 1
    
2.
Mitchell JA, Kirkby NS. Eicosanoids, prostacyclin and cyclooxygenase in the cardiovascular system. Br J Pharmacol 2019;176:1038-50.  Back to cited text no. 2
    
3.
Katzung BG. Basic and Clinical Pharmacology, 14th ed. New York: McGraw Hill Education; 2018. p. 642-5, 703-13.  Back to cited text no. 3
    
4.
Ghasemian M, Owlia S, Owlia MB. Review of anti-inflammatory herbal medicines. Adv Pharmacol Sci 2016;2016:9130979.  Back to cited text no. 4
    
5.
Siddiqui MZ. Boswellia serrata, a potential anti-inflammatory agent: an overview. Indian J Pharm Sci 2011;73:255-61.  Back to cited text no. 5
[PUBMED]  [Full text]  
6.
Pandey P, Tiwari S. Therapeutic potential of Indian plants for the treatment of rheumatoid arthritis. J Pharmacogn Phytochem 2018;7:37-41.  Back to cited text no. 6
    
7.
Chakradhar NS, Kanaka MB, Sangeetha S, Damodharan N. Herbal medicines and formulation approaches for the treatment of arthritis. Drug Invent Today 2018;10:1617-25.  Back to cited text no. 7
    
8.
Rashan L, Hakkim FL, Idrees M, Essa MM, Velusamy T, Al-Baloshi M, et al. Boswellia gum resin and essential oils: Potential health benefits − An evidence based review. Int J Food Sci Nutr 2019;9:53-71.  Back to cited text no. 8
    
9.
Hussain H, Al-Harrasi A, Csuk R, Shamraiz U, Green IR, Ahmed I, et al. Therapeutic potential of Boswellic acids: A patent review (1990-2015). Expert Opin Ther Pat 2017;27:81-90.  Back to cited text no. 9
    
10.
Roy NK, Parama D, Banik K, Bordoloi D, Devi AK, Thakur KK, et al. An update on pharmacological potential of Boswellic acids against chronic diseases. Int J Mol Sci 2019;20:4101.  Back to cited text no. 10
    
11.
Iram F, Khan SA, Husain A. Phytochemistry and potential therapeutic actions of Boswellic acids: A mini-review. Asian Pac J Trop Biomed 2017;7:513-23.  Back to cited text no. 11
    
12.
Kunnumakkara AB, Banik K, Bordoloi D, Harsha C, Sailo BL, Padmavathi G, et al. Googling the guggul (Commiphora and Boswellia) for prevention of chronic diseases. Front Pharmacol 2018;9:686.  Back to cited text no. 12
    
13.
Kathpalia H, Shreya KK. Topical Nanoemmigel formulation of Boswellia serrata. Int J Pharm Pharm Sci 2018;80:261-7.  Back to cited text no. 13
    
14.
Krüger P, Daneshfar R, Eckert GP, Klein J, Volmer DA, Bahr U, et al. Metabolism of boswellic acids in vitro and in vivo. Drug Metab Dispos 2008;36:1135-42.  Back to cited text no. 14
    
15.
Williams AC, Barry BW. Penetration enhancers. Adv Drug Deliv Rev 2012;64:128-37.  Back to cited text no. 15
    
16.
Lundborg M, Wennberg CL, Narangifard A, Lindahl E, Norlén L. Predicting drug permeability through skin using molecular dynamics simulation. J Control Release 2018;283:269-79.  Back to cited text no. 16
    
17.
Lane ME. Skin penetration enhancers. Int J Pharm 2013;447:12-21.  Back to cited text no. 17
    
18.
Herman A, Herman AP. Essential oils and their constituents as skin penetration enhancer for transdermal drug delivery: A review. J Pharm Pharmacol 2015;67:473-85.  Back to cited text no. 18
    
19.
Hajhashemi V, Sajjadi SE, Zomorodkia M. Antinociceptive and anti-inflammatory activities of bunium persicum essential oil, hydroalcoholic and polyphenolic extracts in animal models. Pharm Biol 2011;49:146-51.  Back to cited text no. 19
    
20.
Barbero AM, Frasch HF. Effect of stratum corneum heterogeneity, anisotropy, asymmetry and follicular pathway on transdermal penetration. J Control Release 2017;260:234-46.  Back to cited text no. 20
    
21.
Som I, Bhatia K, Yasir M. Status of surfactants as penetration enhancers in transdermal drug delivery. J Pharm Bioallied Sci 2012;4:2-9.  Back to cited text no. 21
    
22.
Gupta R, Badhe Y, Rai B, Mitragotri S. Molecular mechanism of the skin permeation enhancing effect of ethanol: A molecular dynamics study. RSC Adv 2020;10:12234-48.  Back to cited text no. 22
    
23.
Čižinauskas V, Elie N, Brunelle A, Briedis V. Skin penetration enhancement by natural oils for dihydroquercetin delivery. Molecules 2017;22:1536.  Back to cited text no. 23
    
24.
Haq A, Michniak-Kohn B. Effects of solvents and penetration enhancers on transdermal delivery of thymoquinone: Permeability and skin deposition study. Drug Deliv 2018;25:1943-9.  Back to cited text no. 24
    
25.
Waheed A, Aqil M, Ahad A, Imam SS, Moolakkadath T, Iqbal Z, et al. Improved bioavailability of raloxifene hydrochloride using limonene containing transdermal nano-sized vesicles. J Drug Deliv Sci Technol 2019;52:468-76.  Back to cited text no. 25
    
26.
Nastiti CMRR, Ponto T, Mohammed Y, Roberts MS, Benson HAE. Novel nanocarriers for targeted topical skin delivery of the antioxidant resveratrol. Pharmaceutics 2020;12:108.  Back to cited text no. 26
    
27.
Baek JS, Pham CV, Myung CS, Cho CW. Tadalafil-loaded nanostructured lipid carriers using permeation enhancers. Int J Pharm 2015;495:701-9.  Back to cited text no. 27
    
28.
Akram MR, Ahmad M, Abrar A, Sarfraz RM, Mahmood A. Formulation design and development of matrix diffusion controlled transdermal drug delivery of glimepiride. Drug Des Devel Ther 2018;12:349-64.  Back to cited text no. 28
    
29.
Biruss B, Kahlig H, Valenta C. Evaluation of a eucalyptus oil containing topical drug delivery system for selected steroid hormones. Int J Pharm 2007;328:142-51.  Back to cited text no. 29