Evaluation avocado soybean unsaponifiables loaded in poly (lactic-co-glycolic) acid/avocado soybean unsaponifiables-fibrin nanoparticles scaffold (new delivery system) is an effective factor for tissue engineering

Gorji, Mona; Zargar Kharazi, Anoosheh; Setayeshmehr, Mohsen; Ghasemi, Nazem; Soleimani, Mitra; Hashemibeni, Batool

Evaluation avocado soybean unsaponifiables loaded in poly (lactic-co-glycolic) acid/avocado soybean unsaponifiables-fibrin nanoparticles scaffold (new delivery system) is an effective factor for tissue engineering

Document Type : Original Article

Authors

¹ Skin Research Center, Shahid Beheshti University of Medical Science, Tehran; Department of Anatomical Science, Isfahan Medical University of Medical Science, Isfahan, Iran

² Department of Advanced Medical Technology, Biomaterials Nanaotechnology and Tissue Engineering Group, Isfahan University of Medical Sciences, Isfahan, Iran

³ Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Growth factors and chemical stimulants have key role in cartilage tissue engineering, but these agents have unfavorable effects on cells. Avocado soybean unsaponifiables (ASU) has chondroprotective and anti-inflammatory effects. In this study, fibrin2nanoparticles (FNP)/ASU, as a new delivery system, with stem cells applied for cartilage tissue engineering in poly (lactic-co-glycolic) acid (PLGA) scaffold. Materials and Methods: FNP/ASU prepared by freeze milling and freeze drying. NFP/ASU was characterized by dynamic light scattering (DLS). PLGA-NFP/ASU scaffold was fabricated and assessed by scanning electron microscope (SEM). Human adipose-derived stem cells (hADSCs) were seeded on scaffold and induced for chondrogenesis. After 14 days, cell viability and gene/protein expression evaluated. Results: The results of DLS and SEM indicated that nanoparticles had high quality. The expression of type II collagen and SOX9 and aggrecan (ACAN) genes in differentiated cells in the presence of ASU was significantly increased compared with the control group (P and lt; 0.01), on the other hand, type I collagen expression was significantly decreased and western blot confirmed it. Conclusions: This study indicated FNP/ASU loaded in PLGA scaffold has excellent effect on chondrogenic differentiation of hADSCs and tissue engineering.

Keywords

References

1.	Sutherland AJ, Beck EC, Dennis SC, Converse GL, Hopkins RA, Berkland CJ, et al. Decellularized cartilage may be a chondroinductive material for osteochondral tissue engineering. PLoS One 2015;10:e0121966.
2.	Hardingham T, Tew S, Murdoch A. Tissue engineering: Chondrocytes and cartilage. Arthritis Res 2002;4 Suppl 3:S63-8.
3.	Alipour R, Sadeghi F, Hashemi-Beni B, Zarkesh-Esfahani SH, Heydari F, Mousavi SB, et al. Phenotypic characterizations and comparison of adult dental stem cells with adipose-derived stem cells. Int J Prev Med 2010;1:164-71.
4.	Valiani A, Hashemibeni B, Esfandiary E, Ansar MM, Kazemi M, Esmaeili N. Study of carbon nano-tubes effects on the chondrogenesis of human adipose derived stem cells in alginate scaffold. Int J Prev Med 2014;5:825-34.
5.	Wang ZC, Sun HJ, Li KH, Fu C, Liu MZ. Icariin promotes directed chondrogenic differentiation of bone marrow mesenchymal stem cells but not hypertrophy in vitro. Exp Ther Med 2014;8:1528-34.
6.	Diekman BO, Rowland CR, Lennon DP, Caplan AI, Guilak F. Chondrogenesis of adult stem cells from adipose tissue and bone marrow: Induction by growth factors and cartilage-derived matrix. Tissue Eng Part A 2010;16:523-33.
7.	Taupin P. Adult neural stem cells, neurogenic niches, and cellular therapy. Stem Cell Rev 2006;2:213-9.
8.	Farrell E, O'Brien FJ, Doyle P, Fischer J, Yannas I, Harley BA, et al. A collagen-glycosaminoglycan scaffold supports adult rat mesenchymal stem cell differentiation along osteogenic and chondrogenic routes. Tissue Eng 2006;12:459-68.
9.	Li Z, Kupcsik L, Yao SJ, Alini M, Stoddart MJ. Chondrogenesis of human bone marrow mesenchymal stem cells in fibrin–polyurethane composites. Tissue Engineering Part A. 2009;15:1729-37.
10.	Venkatesan J, Jayakumar R, Anil S, Chalisserry EP, Pallela R, Kim SK. Development of alginate-chitosan-collagen based hydrogels for tissue engineering. J Biomater Tissue Eng 2015;5:458-64.
11.	Li B, Yang J, Ma L, Li F, Tu Z, Gao C. Fabrication of poly (lactide-co-glycolide) scaffold filled with fibrin gel, mesenchymal stem cells, and poly (ethylene oxide)-b-poly (L-lysine)/TGF-β1 plasmid DNA complexes for cartilage restoration in vivo. J Biomed Mater Res A 2013;101:3097-108.
12.	Lü JM, Wang X, Marin-Muller C, Wang H, Lin PH, Yao Q, et al. Current advances in research and clinical applications of PLGA-based nanotechnology. Expert Rev Mol Diagn 2009;9:325-41.
13.	Uematsu K, Hattori K, Ishimoto Y, Yamauchi J, Habata T, Takakura Y, et al. Cartilage regeneration using mesenchymal stem cells and a three-dimensional poly-lactic-glycolic acid (PLGA) scaffold. Biomaterials 2005;26:4273-9.
14.	Croll TI, O'Connor AJ, Stevens GW, Cooper-White JJ. Controllable surface modification of poly (lactic-co-glycolic acid) (PLGA) by hydrolysis or aminolysis I: Physical, chemical, and theoretical aspects. Biomacromolecules 2004;5:463-73.
15.	Wang W, Li B, Yang J, Xin L, Li Y, Yin H, et al. The restoration of full-thickness cartilage defects with BMSCs and TGF-beta 1 loaded PLGA/fibrin gel constructs. Biomaterials 2010;31:8964-73.
16.	Hashemibeni B, Pourentezari M, Valiani A, Zamani M, Mardani M. Effect of icariin on the chondrogenesis of human adipose derived stem cells on poly (lactic-co-glycolic) acid/fibrin composite scaffold. Int J Adv Biotechnol Res 2017;8:595-605.
17.	Bosnakovski D, Mizuno M, Kim G, Takagi S, Okumura M, Fujinaga T. Chondrogenic differentiation of bovine bone marrow mesenchymal stem cells (MSCs) in different hydrogels: Influence of collagen type II extracellular matrix on MSC chondrogenesis. Biotechnol Bioeng 2006;93:1152-63.
18.	Kawamura K, Chu CR, Sobajima S, Robbins PD, Fu FH, Izzo NJ, et al. Adenoviral-mediated transfer of TGF-beta1 but not IGF-1 induces chondrogenic differentiation of human mesenchymal stem cells in pellet cultures. Exp Hematol 2005;33:865-72.
19.	Uebersax L, Merkle HP, Meinel L. Insulin-like growth factor I releasing silk fibroin scaffolds induce chondrogenic differentiation of human mesenchymal stem cells. J Control Release 2008;127:12-21.
20.	Newfeld SJ, Wisotzkey RG, Kumar S. Molecular evolution of a developmental pathway: Phylogenetic analyses of transforming growth factor-beta family ligands, receptors and Smad signal transducers. Genetics 1999;152:783-95.
21.	Dickson MC, Martin JS, Cousins FM, Kulkarni AB, Karlsson S, Akhurst RJ. Defective haematopoiesis and vasculogenesis in transforming growth factor-beta 1 knock out mice. Development 1995;121:1845-54.
22.	Christensen R, Bartels EM, Astrup A, Bliddal H. Symptomatic efficacy of avocado-soybean unsaponifiables (ASU) in osteoarthritis (OA) patients: A meta-analysis of randomized controlled trials. Osteoarthritis Cartilage 2008;16:399-408.
23.	Kapur SK, Dos-Anjos Vilaboa S, Llull R, Katz AJ. Adipose tissue and stem/progenitor cells: discovery and development. Clin Plast Surg 2015;42:155-67.
24.	Lippiello L, Nardo JV, Harlan R, Chiou T. Metabolic effects of avocado/soy unsaponifiables on articular chondrocytes. Evid Based Complement Alternat Med 2008;5:191-7.
25.	Green JA, Hirst-Jones KL, Davidson RK, Jupp O, Bao Y, MacGregor AJ, et al. The potential for dietary factors to prevent or treat osteoarthritis. Proc Nutr Soc 2014;73:278-88.
26.	Hashemibeni B, Mardani M, Valiani A, Pourentezari M, Anvari M, Yadegari M, Mangoli E. Effects of avocado/soybean on the chondrogenesis of human adipose-derived stem cells cultured on polylactic-co-glycolic acid/fibrin hybrid scaffold. J Applied Biotechnology Reports 2019;6:145-50.
27.	Boumediene K, Felisaz N, Bogdanowicz P, Galera P, Guillou GB, Pujol JP. Avocado/soya unsaponifiables enhance the expression of transforming growth factor beta1 and beta2 in cultured articular chondrocytes. Arthritis Rheum 1999;42:148-56.
28.	Altinel L, Saritas ZK, Kose KC, Pamuk K, Aksoy Y, Serteser M. Treatment with unsaponifiable extracts of avocado and soybean increases TGF-beta1 and TGF-beta2 levels in canine joint fluid. Tohoku J Exp Med 2007;211:181-6.
29.	Mazieres B, Tempesta C, Tiechard M, Vaguier G. Pathologic and biochemical effects of a lipidic avocado and soya extract on an experimental post-contusive model of OA. Osteoarthritis Cartilage 1993;1:46.
30.	Aubert-Foucher E, Mayer N, Pasdeloup M, Pagnon A, Hartmann D, Mallein-Gerin F. A unique tool to selectively detect the chondrogenic IIB form of human type II procollagen protein. Matrix Biol 2014;34:80-8.
31.	Petros RA, DeSimone JM. Strategies in the design of nanoparticles for therapeutic applications. Nat Rev Drug Discov 2010;9:615-27.
32.	Maheu E, Mazières B, Valat JP, Loyau G, Le Loët X, Bourgeois P, et al. Symptomatic efficacy of avocado/soybean unsaponifiables in the treatment of osteoarthritis of the knee and hip: A prospective, randomized, double-blind, placebo-controlled, multicenter clinical trial with a six-month treatment period and a two-month followup demonstrating a persistent effect. Arthritis Rheum 1998;41:81-91.
33.	Henrotin YE, Deberg MA, Crielaard JM, Piccardi N, Msika P, Sanchez C. Avocado/soybean unsaponifiables prevent the inhibitory effect of osteoarthritic subchondral osteoblasts on aggrecan and type II collagen synthesis by chondrocytes. J Rheumatol 2006;33:1668-78.
34.	Larrosa M, González-Sarrías A, Yáñez-Gascón MJ, Selma MV, Azorín-Ortuño M, Toti S, et al. Anti-inflammatory properties of a pomegranate extract and its metabolite urolithin-A in a colitis rat model and the effect of colon inflammation on phenolic metabolism. J Nutr Biochem 2010;21:717-25.
35.	Tew SR, Li Y, Pothacharoen P, Tweats LM, Hawkins RE, Hardingham TE. Retroviral transduction with SOX9 enhances re-expression of the chondrocyte phenotype in passaged osteoarthritic human articular chondrocytes. Osteoarthritis Cartilage 2005;13:80-9.
36.	Li D, Yuan T, Zhang X, Xiao Y, Wang R, Fan Y, et al. Icariin: A potential promoting compound for cartilage tissue engineering. Osteoarthritis Cartilage 2012;20:1647-56.

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